63 research outputs found
The impact of partially missing communities~on the reliability of centrality measures
Network data is usually not error-free, and the absence of some nodes is a
very common type of measurement error. Studies have shown that the reliability
of centrality measures is severely affected by missing nodes. This paper
investigates the reliability of centrality measures when missing nodes are
likely to belong to the same community. We study the behavior of five commonly
used centrality measures in uniform and scale-free networks in various error
scenarios. We find that centrality measures are generally more reliable when
missing nodes are likely to belong to the same community than in cases in which
nodes are missing uniformly at random. In scale-free networks, the betweenness
centrality becomes, however, less reliable when missing nodes are more likely
to belong to the same community. Moreover, centrality measures in scale-free
networks are more reliable in networks with stronger community structure. In
contrast, we do not observe this effect for uniform networks. Our observations
suggest that the impact of missing nodes on the reliability of centrality
measures might not be as severe as the literature suggests
Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping
To facilitate fine-scale phenotyping of whole specimens, we describe here a set of tissue fixation-embedding, detergent-clearing and staining protocols that can be used to transform excised organs and whole organisms into optically transparent samples within 1–2 weeks without compromising their cellular architecture or endogenous fluorescence. PACT (passive CLARITY technique) and PARS (perfusion-assisted agent release in situ) use tissue-hydrogel hybrids to stabilize tissue biomolecules during selective lipid extraction, resulting in enhanced clearing efficiency and sample integrity. Furthermore, the macromolecule permeability of PACT- and PARS-processed tissue hybrids supports the diffusion of immunolabels throughout intact tissue, whereas RIMS (refractive index matching solution) grants high-resolution imaging at depth by further reducing light scattering in cleared and uncleared samples alike. These methods are adaptable to difficult-to-image tissues, such as bone (PACT-deCAL), and to magnified single-cell visualization (ePACT). Together, these protocols and solutions enable phenotyping of subcellular components and tracing cellular connectivity in intact biological networks
Cleavage modification did not alter blastomere fates during bryozoan evolution
This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.The study was funded by the core budget of the Sars Centre and by The
European Research Council Community’s Framework Program Horizon 2020
(2014–2020) ERC grant agreement 648861 to A
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