Islands as Time Capsules for Genetic Diversity Conservation: The Case of the Giglio Island Mouflon

The use of multidisciplinary approaches of investigation including biological, biogeographical, historical, morphological, and genetic analysis, can be useful in identifying and preserving biodiversity. The present study focuses on the characterisation and conservation of a mouflon population (Ovis gmelini musimon) from the Mediterranean island of Giglio. Here we provide the first molecular data on the Giglio population and compare it with mouflons from Sardinia, Elba, and Corsica using both nuclear and mitochondrial markers. Our results suggest that the Giglio mouflon harbours genetic variability likely of Sardinian origin but not represented in the current Sardinian mouflon diversity. Although not presenting the typical characteristics of an invasive alien species, the Giglio mouflon is being subjected to eradication through culling or trapping and surgical sterilization. The molecular evidence we report highlights that such actions are causing the irremediable loss of ancestral genetic variants of the genus Ovis. Finally, we highlight how a multidisciplinary approach is necessary to aid the conservation and management of the anthropochorous populations of Mediterranean mammals

Land mammal colonisation of islands is a multifactorial product; a critic to the unwise use of models

The colonization of islands by terrestrial mammals has been the topic of a flurry of studies. Contributions normally tend to oversimplify the subject, focusing on specific key factors which are allegedly considered the best to explain why, how and when non volant terrestrial mammals reached islands and which are used to devise models. The subjectivity in the choice of key factors understandably leads to controversy and debate. Insular faunas are the multifactorial result of complex combinations of facts, conditions and processes. Hence, the faunal assemblages of each island should be inquired individually, on a case-by-case basis. Models, by definition, are simplified representations of reality, which turn into oversimplifications when reality is so overly complex as it is in islands; yet models are usually considered the unavoidable starting point of investigations. An evidence-based, multidisciplinary approach, instead, is highly recommended. Besides obvious paleontological evidence, we need to carefully inspect geological and stratigraphical information, climatological factors, sealevel evolution, paleogeographic characteristics, ecological, physiological and environmental factors, behavioral characters and ecological preferences, genetics and densities of colonizing populations, as well as taphonomic, sampling and analytical biases. The lack of some of this indispensable information seriously affects the reliability of interpretations and reconstructions

Molecular DNA identity of the mouflon of Cyprus (Ovis orientalis ophion, Bovidae): Near Eastern origin and divergence from Western Mediterranean conspecific populations

The mouflon population of Cyprus (Ovis orientalis ophion) comprises historically preserved feral descendants of sheep domesticated during the Neolithic. We determined genetic identity of this taxon in order to elucidate its systematic placement and enforce its protection. We used 12 loci of microsatellite DNA to infer genetic relationships between the Cypriot mouflon and either long-time isolated (Corsica, Sardinia) or recently introduced (central Italy) European mouflons (O. o. musimon). We also sequenced the mitochondrial DNA (mtDNA) Cytochrome-b gene to infer the origin of the Cypriot mouflon including many National Centre for Biotechnology Information (NCBI) entries of European and Near Eastern conspecifics. Microsatellites disclosed net divergence between Western Mediterranean and Cypriot mouflon. The latter was included in the highly heterogeneous Near Eastern O. orientalis mtDNA group, Iran representing the most credited region as the source for its ancient introduction to Cyprus. Both international and national legislation protect the mouflon of Cyprus as a wild taxon (O. o. ophion). However, the IUCN Red List of Threatened Species and NCBI include the Cypriot mouflon as subspecies of its respective domestic species, the sheep (O. aries). Unfortunately, people charged with crime against protected mouflon may benefit from such taxonomic inconsistency between legislation and databases, as the latter can frustrate molecular DNA forensic outcomes. Until a definitive light can be shed on Near Eastern O. orientalis systematics, we suggest that the Cypriot mouflon should be unvaryingly referred to as O. o. ophion in order not to impair conservation in the country where it resides

How Nigeria’s 2015 presidential election outcome was forecasted with geodemographics and public sentiment analytics

In 2015, Nigeria held one of the most fiercely contested presidential elections in the nation’s recent democratic history. The outcome of the election was expected to exert significant influence on democratic practices on the African continent. The stiffness of the contest also meant that it was difficult to predict the likely winner of the election. This paper summarizes how an empirical approach was used to forecast the outcome of the election by modeling public sentiment data-set using a geodemographic framework. Results indicate that the main electorates that determined the outcome of the election were situated in thirteen battleground states. Additionally, results showed that two years before the presidential election, Goodluck Jonathan’s public approval ratings on corruption, insecurity, and the economy (the main drivers of the 2015 election) had nose-dived across many of the battleground states. This eventually contributed toward his loss

Dead or alive?: investigating long-distance transport of live fallow deer and their body parts in antiquity

The extent to which breeding populations of fallow deer were established in Roman Europe has been obscured by the possibility that the skeletal remains of the species, in particular Dama foot bones and antlers, were traded over long distances as objects in their own right. This paper sets out to refine our understanding of the evidence for the transportation of living and dead fallow deer in Iron Age and Roman Europe. To achieve this, museum archives containing purportedly early examples of Dama antler were searched, with available specimens sampled for carbon, nitrogen and strontium isotope analyses, and compared with data for archaeological fallow deer from across Europe. Importantly, the resulting isotope values can be interpreted in light of new modern baseline data for fallow deer presented here. Together these multi-isotope results for modern and archaeological fallow deer provide a more critical perspective on the transportation of fallow deer and their body parts in antiquity

Imports and isotopes: a modern baseline study for interpreting Iron Age and Roman trade in fallow deer antlers

The European Fallow deer (Dama dama dama) became extinct in the British Isles and most of continental Europe at the time of the Last Glacial Maximum, with the species becoming restricted to an Anatolian refugium (Masseti et al. 2008). Human-mediated reintroductions resulted in fallow populations in Rhodes, Sicily, Mallorca, Iberia and other parts of western Europe (Sykes et al. 2013). Eventually, the species was brought to Britain by the Romans during the 1st century AD, with a breeding population being established at Fishbourne Roman Palace (Sykes et al. 2011). The human influence on the present-day distribution of the species makes it particularly interesting from a zooarchaeological perspective. This paper describes my MSc research, as part of the AHRC-funded project Dama International: Fallow Deer and European Society 6000 BC–AD 1600, looking at antlers from Iron Age and Roman sites in Britain for evidence of trade in body parts and whether this can be elucidated by a parallel stable isotope study of modern fallow antlers of known provenance

Historic zoology of the European fallow deer, Dama dama dama: evidence from biogeography, archaeology and genetics

The European fallow deer, Dama dama dama (L., 1758) is one of the most widespread cervids, and its distribution has been heavily affected by man. There is evidence of its artificial distribution beyond the natural homeland carried out by man since prehistory. At present, only one wild autochthonous population is reputed to survive in Anatolia, but its census size is dramatically decreasing. This means that a significant portion of the ancestral genetic diversity of this taxon is seriously threatened. This Turkish stock is also regarded as the main source for all the fallow deer exported by humans since Neolithic times. The aim of the present paper is to investigate the extant genetic, biogeographical, and archaeozoological patterns of the fallow deer dispersed in the insular and continental Mediterranean region. The adoption of a multidisciplinary approach provides the opportunity to advance some new hypotheses regarding the origin of the fallow deer populations which may prove particularly important in terms of the conservation and management of the anthropochorous Mediterranean fauna