Free vibrations of simply-supported beam bridges under moving loads: Maximum resonance, cancellation and resonant vertical acceleration

The advent of high-speed railways has raised many concerns regarding the behaviour of bridges. Particularly, the analysis of the free vibrations generated by each load is of great interest because they can possibly accumulate and create resonance phenomena. Regarding simply supported beams, earlier contributions showed that the free vibrations created by a single moving force are of maximum or zero amplitude (cancellation) for certain speeds. In the present paper new closed-form expressions are given for the cancellation speeds of a generic mode, as well as for the most representative points of maximum amplitude. Similar new results are provided for elastically supported beams as well. A simpler, closed-form approximate expression of the cancellation condition for an elastically supported beam is also derived from the analysis of a single passing load; this approximate formula is in good agreement with the exact results. Knowing a priori the speeds of maximum free vibrations or cancellation is of great interest for experimental tests on bridges, particularly as regards the evaluation of amplitude-dependent magnitudes such as structural damping. Regarding the resonance phenomena, if the resonance speeds coincide with either a maximum free vibration or a cancellation speed, then a maximum resonance or a cancellation of resonance will occur. The most relevant cases thereof have been investigated, and new expressions which allow predicting them for a generic mode are given. Finally, a new approximate formula is proposed for estimating the maximum acceleration of simply supported bridges caused by resonances of the fundamental mode. After extensive numerical testing, the formula has proved to be a useful tool for a first assessment of simply supported bridges according to building codes such as Eurocodes. (C) 2012 Elsevier Ltd. All rights reserved.The authors acknowledge the financial support of the State Secretariat for Research of the Spanish Ministry of Science and Innovation (Secretaria de Estado de Investigacion, Ministerio de Ciencia e Innovacion, MICINN) in the framework of the Research Project BIA2008-04111.Museros Romero, P.; Moliner, E.; Martinez-Rodrigo, M. (2013). Free vibrations of simply-supported beam bridges under moving loads: Maximum resonance, cancellation and resonant vertical acceleration. Journal of Sound and Vibration. 332(2):326-345. https://doi.org/10.1016/j.jsv.2012.08.008326345332

Genome Sequence of a Novel Archaeal Fusellovirus Assembled from the Metagenome of a Mexican Hot Spring

The consensus genome sequence of a new member of the family Fuselloviridae designated as SMF1 (Sulfolobales Mexican fusellovirus 1) is presented. The complete circular genome was recovered from a metagenomic study of a Mexican hot spring. SMF1 exhibits an exceptional coding strand bias and a reduced set of fuselloviral core genes

Non-thermal emission in hyper-velocity and semi-relativistic stars

Context. There is a population of runaway stars that move at extremely high speeds with respect to their surroundings. The fast motion and the stellar wind of these stars, plus the wind-medium interaction, can lead to particle acceleration and non-thermal radiation. Aims. We characterise the interaction between the winds of fast runaway stars and their environment, in particular to establish their potential as cosmic-ray accelerators and non-thermal emitters. Methods. We model the hydrodynamics of the interaction between the stellar wind and the surrounding material. We self-consistently calculate the injection and transport of relativistic particles in the bow shock using a multi-zone code, and compute their broadband emission from radio to γ-rays. Results. Both the forward and reverse shocks are favourable sites for particle acceleration, although the radiative efficiency of particles is low and therefore the expected fluxes are in general rather faint. Conclusions. We show that high-sensitivity observations in the radio band can be used to detect the non-thermal radiation associated with bow shocks from hyper-velocity and semi-relativistic stars. Hyper-velocity stars are expected to be modest sources of sub-TeV cosmic rays, accounting perhaps for ∼0.1% of that of galactic cosmic rays

Rings and spirals in barred galaxies. I Building blocks

In this paper we present building blocks which can explain the formation and properties both of spirals and of inner and outer rings in barred galaxies. We first briefly summarise the main results of the full theoretical description we have given elsewhere, presenting them in a more physical way, aimed to an understanding without the requirement of extended knowledge of dynamical systems or of orbital structure. We introduce in this manner the notion of manifolds, which can be thought of as tubes guiding the orbits. The dynamics of these manifolds can govern the properties of spirals and of inner and outer rings in barred galaxies. We find that the bar strength affects how unstable the L1 and L2 Lagrangian points are, the motion within the 5A5A5Amanifold tubes and the time necessary for particles in a manifold to make a complete turn around the galactic centre. We also show that the strength of the bar, or, to be more precise, of the non-axisymmetric forcing at and somewhat beyond the corotation region, determines the resulting morphology. Thus, less strong bars give rise to R1 rings or pseudorings, while stronger bars drive R2, R1R2 and spiral morphologies. We examine the morphology as a function of the main parameters of the bar and present descriptive two dimensional plots to that avail. We also derive how the manifold morphologies and properties are modified if the L1 and L2 Lagrangian points become stable. Finally, we discuss how dissipation affects the manifold properties and compare the manifolds in gas-like and in stellar cases. Comparison with observations, as well as clear predictions to be tested by observations will be given in an accompanying paper.Comment: Typos corrected to match the version in press in MNRA

Halo based reconstruction of the cosmic mass density field

We present the implementation of a halo based method for the reconstruction of the cosmic mass density field. The method employs the mass density distribution of dark matter haloes and its environments computed from cosmological N-body simulations and convolves it with a halo catalog to reconstruct the dark matter density field determined by the distribution of haloes. We applied the method to the group catalog of Yang etal (2007) built from the SDSS Data Release 4. As result we obtain reconstructions of the cosmic mass density field that are independent on any explicit assumption of bias. We describe in detail the implementation of the method, present a detailed characterization of the reconstructed density field (mean mass density distribution, correlation function and counts in cells) and the results of the classification of large scale environments (filaments, voids, peaks and sheets) in our reconstruction. Applications of the method include morphological studies of the galaxy population on large scales and the realization of constrained simulations.Comment: Accepted for publication in MNRA

Mitogen-activated protein kinase phosphatase-3 (MKP-3) in the surgical wound is necessary for the resolution of postoperative pain in mice

Mitogen-activated protein kinase (MAPK) phosphatase-3 (MKP-3) and its substrates (extracellular signal-regulated kinase [ERK] and p38) play an important role in pathophysiological mechanisms of acute postoperative and chronic neuropathic pain in the spinal cord. This study aimed to understand the role of MKP-3 and its target MAPKs at the site of surgical incision in nociceptive behavior. Wild-type (WT) and MKP-3 knockout (KO) mice underwent unilateral plantar hind paw incision. Mechanical allodynia was assessed by using von Frey filaments. Peripheral ERK-1/2 and p38 phosphorylation were measured by Western blot. Cell infiltration was determined using hematoxylin and eosin histological staining. Peripheral phosphorylated ERK-1/2 (p-ERK-1/2) inhibition was performed in MKP-3 KO mice. In WT mice, mechanical hypersensitivity was observed on postoperative day 1 (0.69±0.17 g baseline vs 0.13±0.08 g day 1), which resolved normally by postoperative day 12 (0.46±0.08 g, N=6). In MKP-3 KO mice, this hypersensitivity persisted at least 12 days after surgery (0.19±0.06 g; N=6). KO mice displayed higher numbers of infiltrating cells (51.4±6 cells/0.1 mm(2)) than WT mice (8.7±1.2 cells/0.1 mm(2)) on postoperative day 1 (vs 5–6 cells/0.1 mm(2) at baseline) that returned to baseline 12 days after surgery (10–12 cells/0.1 mm(2)). In WT mice, peripheral p-p38 and p-ERK-1/2 expression increased (5- and 3-fold, respectively) on postoperative days 1 and 5, and returned to basal levels 7–12 days after surgery (N=3 per group). Peripheral p-p38 levels in MKP-3 KO mice followed a similar expression pattern as WT mice. Peripheral p-ERK-1/2 levels in MKP-3 KO mice remained elevated 12 days after surgery (2.5-fold, N=3 per group). Administration of PD98059 (MEK inhibitor, N=8, vehicle N=9) reduced p-ERK-1/2 expression in the incised tissue and blocked hypersensitivity in MKP-3 KO mice (N=6). The findings of this study suggest that MKP-3 is pivotal for normal resolution of acute postoperative allodynia, through the regulation of peripheral p-ERK-1/2

Excess abdominal fat is associated with cutaneous allodynia in individuals with migraine: a prospective cohort study

Objective: To investigate the specific relationship between cutaneous allodynia (CA) and the percentages of body fat (BF) and abdominal fat in migraineurs. Additionally, we compared serum levels of inflammatory biomarkers in patients with and without CA. Background: Excess abdominal fat might facilitate progressive changes in nociceptive thresholds causing central sensitization, clinically reflected as CA, which could drive migraine progression. Methods: This prospective cohort study included 80 patients with migraine (mean age 39 years, 81.2% female) and 39 non-migraine controls. We analysed each participant’s height, body weight, and body mass index (BMI). The amount and distribution of BF was also assessed by air displacement plethysmography (ADP) and ViScan, respectively. We analysed serum levels of markers of inflammation, during interictal periods. Results: We studied 52 patients with episodic migraine (EM) and 28 with chronic migraine (CM). Of the 80 patients, 53 (53.8%) had CA. Migraineurs with CA had a higher proportion of abdominal fat values than patients without CA (p = 0.04). The independent risk factors for CA were the use of migraine prophylaxis (OR 3.26, 95% CI [1.14 to 9.32]; p = 0.03), proportion of abdominal fat (OR 1.13, 95% CI [1.01 to 1.27]; p = 0.04), and presence of sleep disorders (OR 1.13, 95% CI [00.01 to 1.27]; p = 0.04). The concordance correlation coefficient between the ADP and BMI measurements was 0.51 (0.3681 to 0.6247). CA was not correlated with the mean plasma levels of inflammatory biomarkers. Conclusions: There is a relation between excess abdominal fat and CA. Abdominal obesity might contribute to the development of central sensitization in migraineurs, leading to migraine chronification

Mitogen-Activated Protein Kinase Phosphatase-3 (MKP-3) in the Surgical Wound is Necessary for the Resolution of Postoperative Pain in Mice

Mitogen-activated protein kinase (MAPK) phosphatase-3 (MKP-3) and its substrates (extracellular signal-regulated kinase [ERK] and p38) play an important role in pathophysiological mechanisms of acute postoperative and chronic neuropathic pain in the spinal cord. This study aimed to understand the role of MKP-3 and its target MAPKs at the site of surgical incision in nociceptive behavior. Wild-type (WT) and MKP-3 knockout (KO) mice underwent unilateral plantar hind paw incision. Mechanical allodynia was assessed by using von Frey filaments. Peripheral ERK-1/2 and p38 phosphorylation were measured by Western blot. Cell infiltration was determined using hematoxylin and eosin histological staining. Peripheral phosphorylated ERK-1/2 (p-ERK-1/2) inhibition was performed in MKP-3 KO mice. In WT mice, mechanical hypersensitivity was observed on postoperative day 1 (0.69±0.17 g baseline vs 0.13±0.08 g day 1), which resolved normally by postoperative day 12 (0.46±0.08 g, N=6). In MKP-3 KO mice, this hypersensitivity persisted at least 12 days after surgery (0.19±0.06 g; N=6). KO mice displayed higher numbers of infiltrating cells (51.4±6 cells/0.1 mm2) than WT mice (8.7±1.2 cells/0.1 mm2) on postoperative day 1 (vs 5-6 cells/0.1 mm2 at baseline) that returned to baseline 12 days after surgery (10-12 cells/0.1 mm2). In WT mice, peripheral p-p38 and p-ERK-1/2 expression increased (5- and 3-fold, respectively) on postoperative days 1 and 5, and returned to basal levels 7-12 days after surgery (N=3 per group). Peripheral p-p38 levels in MKP-3 KO mice followed a similar expression pattern as WT mice. Peripheral p-ERK-1/2 levels in MKP-3 KO mice remained elevated 12 days after surgery (2.5-fold, N=3 per group). Administration of PD98059 (MEK inhibitor, N=8, vehicle N=9) reduced p-ERK-1/2 expression in the incised tissue and blocked hypersensitivity in MKP-3 KO mice (N=6). The findings of this study suggest that MKP-3 is pivotal for normal resolution of acute postoperative allodynia, through the regulation of peripheral p-ERK-1/2

Circulating exosomes deliver free fatty acids from the bloodstream to cardiac cells:Possible role of CD36

Regulation of circulating free fatty acid (FFA) levels and delivery is crucial to maintain tissue homeostasis. Exosomes are nanomembranous vesicles that are released from diverse cell types and mediate intercellular communication by delivering bioactive molecules. Here, we sought to investigate the uptake of FFAs by circulating exosomes, the delivery of FFA-loaded exosomes to cardiac cells and the possible role of the FFA transporter CD36 in these processes. Circulating exosomes were purified from the serum of healthy donors after an overnight fast (F) or 20 minutes after a high caloric breakfast (postprandial, PP). Western blotting, Immunogold Electron Microscopy and FACS analysis of circulating exosomes showed that CD36 was expressed under both states, but was higher in postprandial-derived exosomes. Flow cytometry analysis showed that circulating exosomes were able to take-up FFA directly from serum. Importantly, preincubation of exosomes with a blocking CD36 antibody significantly impeded uptake of the FFA analogue BODIPY, pointing to the role of CD36 in FFA exosomal uptake. Finally, we found that circulating exosomes could delivery FFA analogue BODIPY into cardiac cells ex vivo and in vivo in a mice model. Overall, our results suggest a novel mechanism in which circulating exosomes can delivery FFAs from the bloodstream to cardiac tissue. Further studies will be necessary to understand this mechanism and, in particular, its potential involvement in metabolic pathologies such as obesity, diabetes and atherosclerosis