100 research outputs found

    Effects of artificial feeds on growth and production of fishes in Polyculture

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    A study on the effects of artificial feeds on growth and production of fishes along with some limnological conditions were conducted in polyculture system. Species of Indian major carp (Cirrhinus mrigala) and exotic fishes (Hypophthalmicthys molitrix and Oreochromis niloticus) were stocked in six ponds under two treatments, each with three replications. Stocking rate in both treatments was 100 fish per decimal at the ratio of silver carp: tilapia: mrigal = 2: 2: 1. Fertilization and artificial feeds were given in Ttreatment 1 (T1) and only fertilization was done in Treatment 2 (T2). Wheat bran, rice bran and soybean meal were given daily as artificial feed in T1 in the ratio of wheat bran: rice bran: soybean meal = 2: 2: 1 (by wt). Urea, T.S.P and cow dung were applied fortnightly at the rate of 60 g deci-1, 90 g deci-1 and 2 kg deci-1 respectively. Water temperature, transparency, pH, dissolved oxygen, free CO2, total alkalinity, PO4-P and NO3-N were determined fortnightly and phytoplankton and zooplankton were studied fortnightly. These limnological conditions were more or less similar in the ponds under two treatments and were within suitable ranges. Calculated gross and net yields of fish were 16.56 and 12.48 ton ha-1 respectively in case of fertilization and artificial feeding application (T1) and 9.99 and 5.91 ton ha-1 respectively in case of only fertilization (T2). Application of artificial feed in T1 significantly increased the growth and production of fish more than two times which indicates that artificial feeding in polyculture is very useful for increasing fish production. DOI: http://dx.doi.org/10.3329/ijarit.v4i2.22637 Int. J. Agril. Res. Innov. & Tech. 4 (2): 11-15, December, 201

    Effects of different kinds of fertilizers on production of fishes in Polyculture system

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    A study was conducted to assess the effects of different kinds of inorganic fertilizers on the production of fishes in six ponds during October to December 2011. There were three treatments with two replications under each treatment and each of the ponds was stocked with 80 fish fry. In treatments I, II and III, ponds were fertilized fortnightly @ urea 100 g decimal-1, T.S.P. 100 g decimal-1 and urea 50 g decimal-1 + T.S.P. 50 g decimal-1, respectively. Selected water-quality parameters of ponds under study were more or less similar and within the productive range. Mean phytoplankton and zooplankton densities under treatments I, II and III were 57.08 ± 1.35, 8.80 ± 0.09 and 77.29 ± 3.72, 12.88 ± 0.74 and 98.93 ± 1.61, 16.16 ± 1.75 (x103) cells L-1, respectively. The net and gross fish productions of the ponds under treatments I, II and III were 0.85 and 3.11 t ha-1 yr-1 and 1.32 and 3.58 t ha-1 yr-1 and 1.85 and 4.11 t ha-1 yr-1, respectively. Fish production under treatment III was better than those under treatments I and II because plankton population densities under treatment III was the highest. Therefore, the mixed fertilization is suitable for production of plankton that enhance growth and production of fishes. DOI: http://dx.doi.org/10.3329/ijarit.v4i2.22639 Int. J. Agril. Res. Innov. & Tech. 4 (2): 16-21, December, 201

    Ductility Enhancement of Sustainable Fibrous-Reinforced High-Strength Lightweight Concrete

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    To limit the cross-sectional size of concrete structures, high-strength, lightweight concrete is preferred for the design and construction of structural elements. However, the main drawback of high-strength, lightweight concrete is its brittleness over normal-weight concrete. The ductility of concrete is a crucial factor, which plays an important role when the concrete structures are subjected to extreme situations, such as earthquakes and wind. This study aims to improve the ductility of high-strength, lightweight concrete by incorporating steel fibers. The palm oil clinker (POC)-based, high-strength, lightweight concrete specimens reinforced with steel fibers were prepared and their ductility was systematically examined. POC was used as aggregates and supplementary cementitious materials. Steel fibers from 0–1.50% (by volume), with an increment of 0.5%, were used in the concrete mix. Compression ductility, displacement ductility and energy ductility were used as indicators to evaluate the enhancement of ductility. Moreover, the compressive strength, flexural strength, strain–strain behavior, modulus of elasticity, load-displacement characteristics, energy absorption capacity and deformability of the concrete samples were investigated. The compression ductility, displacement ductility and energy ductility indexes were found to be increased by up to 472%, 140% and 568% compared to the control specimens (concrete with 0% steel fibers), respectively. Moreover, the deformability and energy absorption capacity of the concrete were increased by up to 566% and 125%, respectively. Therefore, POC-based, high-strength, fibrous, lightweight concrete could perform better than conventional concrete under extreme loading conditions as it showed significantly higher ductility

    Psychometric validation of the Bangla fear of COVID-19 Scale: confirmatory factor analysis and Rasch analysis

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    The recently developed Fear of COVID-19 Scale (FCV-19S) is a seven-item uni-dimensional scale that assesses the severity of fears of COVID-19. Given the rapid increase of COVID-19 cases in Bangladesh, we aimed to translate and validate the FCV-19S in Bangla. The forward-backward translation method was used to translate the English version of the questionnaire into Bangla. The reliability and validity properties of the Bangla FCV-19S were rigorously psychometrically evaluated (utilizing both confirmatory factor analysis and Rasch analysis) in relation to socio-demographic variables, national lockdown variables, and response to the Bangla Health Patient Questionnaire. The sample comprised 8550 Bangladeshi participants. The Cronbach α value for the Bangla FCV-19S was 0.871 indicating very good internal reliability. The results of the confirmatory factor analysis showed that the uni-dimensional factor structure of the FCV-19S fitted well with the data. The FCV-19S was significantly correlated with the nine-item Bangla Patient Health Questionnaire (PHQ-90) (r = 0.406,

    Wt1 is required for cardiovascular progenitor cell formation through transcriptional control of Snail and E-cadherin

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    Epicardial epithelial-mesenchymal transition (EMT) is hypothesized to generate cardiovascular progenitor cells that differentiate into various cell types, including coronary smooth muscle and endothelial cells, perivascular and cardiac interstitial fibroblasts and cardiomyocytes. Here we show that an epicardial-specific knockout of Wt1 leads to a reduction of mesenchymal progenitor cells and their derivatives. We demonstrate that Wt1 is essential for repression of the epithelial phenotype in epicardial cells and during Embryonic Stem (ES) cell differentiation, through direct transcriptional regulation of Snail (Snai1) and E-cadherin (Cdh1), two of the major mediators of EMT. Some mesodermal lineages fail to form in Wt1 null embryoid bodies but this effect is rescued by the expression of Snai1, underlining the importance of EMT in generating these differentiated cells. These new insights into the molecular mechanisms regulating cardiovascular progenitor cells and EMT will shed light on the pathogenesis of heart diseases and may help the development of cell based therapies

    Self-renewal and chemotherapy resistance of p75NTR positive cells in esophageal squamous cell carcinomas

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    <p>Abstract</p> <p>Background</p> <p>p75<sup>NTR </sup>has been used to isolate esophageal and corneal epithelial stem cells. In the present study, we investigated the expression of p75<sup>NTR </sup>in esophageal squamous cell carcinoma (ESCC) and explored the biological properties of p75<sup>NTR+ </sup>cells.</p> <p>Methods</p> <p>p75<sup>NTR </sup>expression in ESCC was assessed by immunohistochemistry. p75<sup>NTR+ </sup>and p75<sup>NTR- </sup>cells of 4 ESCC cell lines were separated by fluorescence-activated cell sorting. Differentially expressed genes between p75<sup>NTR+ </sup>and p75<sup>NTR- </sup>cells were determined by real-time quantitative reverse transcription-PCR. Sphere formation assay, DDP sensitivity assay, <sup>64</sup>copper accumulation assay and tumorigenicity analysis were performed to determine the capacity of self-renewal, chemotherapy resistance and tumorigenicity of p75<sup>NTR+ </sup>cells.</p> <p>Results</p> <p>In ESCC specimens, p75<sup>NTR </sup>was found mainly confined to immature cells and absent in cells undergoing terminal differentiation. The percentage of p75<sup>NTR+ </sup>cells was 1.6%–3.7% in Eca109 and 3 newly established ESCC cell lines. The expression of Bmi-1, which is associated with self-renewal of stem cells, was significantly higher in p75<sup>NTR+ </sup>cells. p63, a marker identified in keratinocyte stem cells, was confined mainly to p75<sup>NTR+ </sup>cells. The expression of CTR1, which is associated with cisplatin (DDP)-resistance, was significantly decreased in p75<sup>NTR+ </sup>cells. Expression levels of differentiation markers, such as involucrin, cytokeratin 13, ÎČ1-integrin and ÎČ4-integrin, were lower in p75<sup>NTR+ </sup>cells. In addition, p75<sup>NTR+ </sup>cells generated both p75<sup>NTR+ </sup>and p75<sup>NTR- </sup>cells, and formed nonadherent spherical clusters in serum-free medium supplemented with growth factors. Furthermore, p75<sup>NTR+ </sup>cells were found to be more resistant to DDP and exhibited lower <sup>64</sup>copper accumulation than p75<sup>NTR- </sup>cells.</p> <p>Conclusion</p> <p>Our results demonstrated that p75<sup>NTR+ </sup>cells possess some characteristics of CSCs, namely, self-renewal and chemotherapy resistance. Chemotherapy resistance of p75<sup>NTR+ </sup>cells may probably be attributable to decreased expression of CTR1.</p
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