An assessment of genetic variation in Stereum hirsutum (Basidiomycota) based on RAPD markers

Randomly amplified polymorphic DNA (RAPD) marking with five decamer primers (OPA-01, OPA-04, P36, P46, P49) was applied to 41 samples, which were fruitbody tissue isolates of corticioid wood decay fungus S. hirsutum from 18 collection sites in Belarus, situated 0.18–278 km apart. Each primer produced a unique fingerprint for each individual genotype. The samples belong to 35 different genotypes; the identity of RAPD profiles was observed for the samples collected from the same substratum unit. Among 65 reproducible RAPD loci selected for the analysis, there were no strictly monomorphic ones; a single marker had very high frequency (0.94); 29% of markers had the frequencies more than 0.5; the average marker frequency was 0.36. Cluster analysis showed no groups correlated with host, substratum type, or geographical origin, except 3 genotypes from the same collection site, forming a separate cluster. A collection site with the biggest subsample (26% of all studied genotypes) possessed 85% of bands and 100% of them were polymorphic inside this subsample. Average Dice similarity coefficient was almost identical between the genotypes within collection sites (less 0.15 km apart; SD =0.53) and for the widely separated individuals (more 40 km; SDD=0.52). The molecular data infer highly outcrossing reproduction mode in the studied regional sampling of S. hirsutum and the absence of any genetic differentiation

Analysis of genetic variation in Chondrostereum рurpureum (Basidiomycetes) based on RAPD markers

Fifteen samples (fruitbody tissue isolates) of Ch. purpureum, collected throughout Minsk City, were subjected to RAPD-PCR with 3 primers of OPA series and 7 priers of Pseries. Each sample/prim er pair produced unique fingerprint, which permitted to distinguish 10 different genotypes from 10 separate substratum units. The analysis included 64 RAPD loci, obtained with 5 primers; 14% of the loci was monomorphic; 34% of polymorphic loci had the frequency 0.5 and more. Small spatial clusters of individuals (subpopulations) bore 70-75% of the bands, involved in the analysis; 39-47% of the all studied loci only were polymorphic within these subpopulations. Dice similarity coefficients between the genotypes were 0.58-0.83 in the studied sample, which was generally in agreement with the data on Ch. purpureum in Canada. The genetic variation of Ch. purpureum in Minsk City was lower, than among individuals of ecologically close basidiomycete Stereum hirsutum,collected from the same area and tested by the same method