c-Fos expression induced by electroacupuncture at the Zusanli point in rats submitted to repeated immobilization

In laboratory animals, acupuncture needs to be performed on either anesthetized or, if unanesthetized, restrained subjects. Both procedures up-regulate c-Fos expression in several areas of the central nervous system, representing therefore a major pitfall for the assessment of c-Fos expression induced by electroacupuncture. Thus, in order to reduce the effect of acute restraint we used a protocol of repeated restraint for the assessment of the brain areas activated by electroacupuncture in adult male Wistar rats weighing 180-230 g. Repeated immobilization protocols (6 days, 1 h/day and 13 days, 2 h/day) were used to reduce the effect of acute immobilization stress on the c-Fos expression induced by electroacupuncture at the Zusanli point (EA36S). Animals submitted to immobilization alone or to electroacupuncture (100 Hz, 2-4 V, faradic wave) in a non-point region were compared to animals submitted to electroacupuncture at EA36S (4 animals/subgroup). c-Fos expression was measured in 41 brain areas by simple counting of cells and the results are reported as number of c-Fos-immunoreactive cells/10,000 µm². The protocols of repeated immobilization significantly reduced the immobilization-induced c-Fos expression in most of the brain areas analyzed (P < 0.05). Animals of the EA36S groups had significantly higher levels of c-Fos expression in the dorsal raphe nucleus, locus coeruleus, posterior hypothalamus and central medial nucleus of the thalamus. Furthermore, the repeated immobilization protocols intensified the differences between the effects of 36S and non-point stimulation in the dorsal raphe nucleus (P < 0.05). These data suggest that high levels of stress can interact with and mask the evaluation of specific effects of acupuncture in unanesthetized animals.Universidade Federal Rural do Rio de Janeiro Departamento de Ciências FisiológicasUniversidade de São Paulo Instituto de Ciências Biomédicas Departamento de Fisiologia e BiofísicaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de PsicobiologiaUniversidade Federal de São Paulo (UNIFESP) Escola Paulista de Medicina Departamento de FisiologiaUNIFESP, EPM, Depto. de PsicobiologiaUNIFESP, EPM, Depto. de FisiologiaSciEL

Nevoid Basal-cell Syndrome: Literature Review And Case Report In A Family.

The Nevoid Basal-Cell Carcinoma Syndrome (NBCC), or as it is also referred to, basal-cell nevus syndrome or Gorlin-Goltz syndrome, is characterized by multiple early-appearing basal cell carcinomas, keratocytosis of the mandible, and anomalies of the ocular, skeletal reproductive system. We describe four patients in the same family, all of them possessing a large number of skin tumors associated with other typical clinical and X-Ray anomalies of NBCC. The definitive treatment of NBCC has yet to be established, however, early diagnosis is very important as well as the periodical follow-up examination of ten patients, mainly due to the transformations in the skin lesions that may occur.113391792

Novos Isolados De Trichoderma Antagônicos A Sclerotinia Sclerotiorum

Forty-nine isolates of Trichoderma from the Brazilian Midwest were evaluated for their antagonistic activity in vitro against Sclerotinia sclerotiorum (causal agent of white mold), which were then identified based on their nuclear ribosomal ITS sequences. Paired culture tests showed that all isolates exhibited some antagonism, with a maximum of 77% mycelial inhibition and complete inhibition of sclerotia production. Two isolates were found to be the most promising biocontrol agents, considering both antagonistic parameters (CEN1253 - T. koningiopsis and CEN1265 - T. brevicompactum). Five different species were identified: T. harzianum (23), T. spirale (9), T. koningiopsis (8), T. brevicompactum (7) and T. asperellum (2). These isolates are stored in the Embrapa Fungi Collection for Biological Control and the information obtained in the experiments will be incorporated into the database of biological assets within the genetic resources information system (Allele) and be made available for further studies. © 2016, Universidade Estadual de Campinas UNICAMP. All rights reserved.16

Large chestnut trees did not respond to annual fertiliser applications, requiring a long-term approach to establishing effective fertilisation plans

Due to the high value of the fruit, the European chestnut (Castanea sativa Mill.), usually grown in agroforestry systems, has been planted as a single species in orchards managed with increasingly intensive cropping practices, such as the regular use of fertilisers. This justifies research into establishing fertilisation programmes oriented towards ecological intensification. In this study, the results of fruit production, plant nutritional status and soil properties are reported from a field trial in which three NPK fertilisers (20:7:10, 13:11:21 and 7:14:14) and a control treatment were used. Chestnut yields did not vary significantly between treatments, although the mean values of the control showed a clear downward trend. N supplied by the fertilisers seems to have been the most important factor in the difference between the fertilised and control treatments, since leaf N concentrations were lower in the control and often below the lower limit of the sufficiency range. Soil inorganic N levels in the autumn, and tissue N concentrations of the herbaceous vegetation developing beneath the trees, indicated risks of N loss to the environment and highlighted the importance of this vegetation remaining during the winter. The chestnuts’ poor response to fertiliser applications was attributed to the buffering effect of the large perennial structure of the trees on the distribution of nutrients to the growing plant parts. In large trees, it seems appropriate to base the annual fertilisation plan on leaf nutrient concentration. Thus, farmers probably should avoid spending money on fertilizer applications as long as leaf nutrient concentrations do not approach the lower limits of sufficiency ranges.This work was funded by the Operational Group “EGIS–Estratégias de Gestão Integrada do Solo e da Água em Espécies Produtoras de Frutos Secos”, funded by PT2020 and EAFRD (European Agricultural Fund for Rural Development). The authors are also grateful to the Foundation for Science and Technology (FCT, Portugal) for financial support through national funds FCT/MCTES (PIDDAC) to CIMO (UIDB/00690/2020 and UIDP/00690/2020), SusTEC (LA/P/0007/2021) and CITAB (UIDB/04033/2020)info:eu-repo/semantics/publishedVersio

The Collective Field Theory of a Singular Supersymmetric Matrix Model

The supersymmetric collective field theory with the potential $v'(x)=\omega x-{\eta\over x}$ is studied, motivated by the matrix model proposed by Jevicki and Yoneya to describe two dimensional string theory in a black hole background. Consistency with supersymmetry enforces a two band solution. A supersymmetric classical configuration is found, and interpreted in terms of the density of zeros of certain Laguerre polynomials. The spectrum of the model is then studied and is seen to correspond to a massless scalar and a majorana fermion. The $x$ space eigenfunctions are constructed and expressed in terms of Chebyshev polynomials. Higher order interactions are also discussed.Comment: Revtex 8 pages, Submitted to Phys. Rev. D. References and preprint numbers have been adde

Clinical outcomes of patients with acute myeloid leukemia: evaluation of genetic and molecular findings in a real-life setting

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Adubação mineral de Pinus oocarpa schiede

O presente ensaio refere-se à adubação de Pinus oocarpa Schiede. Foi instalado em solo Latossolo Vermelho Escuro Distrófico originalmente sob vegetação de cerrado, no município de Assis, São Paulo. Através de medições periódicos de altura e DAP (diâmetro à altura do peito) das árvores, foram verificados os efeitos da adubação NPK, calagem, S, B e Zn sob delineamento experimental de blocos casualizados com quatro repetições. Pela análise estatística dos últimos dados de altura e diâmetro, quando as plantas estavam com 3 anos, conclui-se que: a adubação NPK proporcionou um aumento significativo no desenvolvimento das plantas em altura e diâmetro; a adubação NPK + calagem aumentou significativamente a altura; a adubação NPK + calagem + S aumentou significativamente o diâmetro e a aplicação de B e Zn não apresentou efeitos significativos.The present work deals with the mineral fertilization of Pinus oocarpa Schiede. The experiment took place in a Distrophic Dark-Red Latosol, formerly under "cerrado" vegetation in Assis, State of São Paulo, Brazil. The height and D.B.C. (diameter breast height) of the trees were periodically measured in order to check the effects of NPK, liming, S, B and Zn fertilization under experimental delineation of randomized blocks with four repetitions . By means of statistical analysis of the last height and diameter data on three year-old plants, we can conclude that: NPK fertilization significantly increased the height and diameter of the plants; NPK + liming treatment significantly increased the plants height; NPK + liming + S treatment significantly increased the plants diameter and the application of B and Zn did not exert any effect

Potential of elephant grass genotypes silages as exclusive roughage on tissue composition and meat quality of lambs: a preliminary study

ABSTRACT This study aimed to evaluate the effects of diets containing elephant grass genotypes silages as exclusive roughage on leg tissue composition, and physicochemical characteristics of meat of lambs. Twenty-four crossbred male lambs with an average initial body weight of 20.29±2.66kg were distributed in a complete randomized design with three treatments and eight replicates. The treatments consisted of three silages of elephant grass genotypes (IRI-381, Elephant B or Mott), without additives or wilting, as the only roughage. The diets did not affect (P>0.05) the dry matter (898.70±60.10 g/day), crude protein (128.93±6.91g/day), total digestible nutrients (690.20±91.82g/day) intakes, body weight at slaughter (24.83±2.79kg), and carcass yields (P>0.05). The tissue composition of the leg did not differ significantly between silages of elephant grass genotypes (P>0.05). No difference (P>0.05) for the physicochemical characteristics of meat from lambs fed diets tested was observed. Therefore, our results indicate that diets containing 50% elephant grass genotypes silages (IRI-381, Elephant B or Mott), harvested at 60 days of growth, have potential for use in lambs feeding

Bottom Production

We review the prospects for bottom production physics at the LHC.Comment: 74 pages, Latex, 71 figures, to appear in the Report of the ``1999 CERN Workshop on SM physics (and more) at the LHC'', P. Nason, G. Ridolfi, O. Schneider G.F. Tartarelli, P. Vikas (conveners

Fokker-Planck equations and density of states in disordered quantum wires

We propose a general scheme to construct scaling equations for the density of states in disordered quantum wires for all ten pure Cartan symmetry classes. The anomalous behavior of the density of states near the Fermi level for the three chiral and four Bogoliubov-de Gennes universality classes is analysed in detail by means of a mapping to a scaling equation for the reflection from a quantum wire in the presence of an imaginary potential.Comment: 10 pages, 5 figures, revised versio