Bacteriological study of the liver of cattle for dicroceliosis

The article presents the results of organoleptic and microbiological studies of the chilled liver of cattle affected by the helminth Dicrocoelium lanceatum. The main goal was to determine how temperature regimes affect the quality and safety of the liver of cattle affected by dicrocelia and its contamination with microflora. An organoleptic examination of all animal slaughter products was carried out. According to organoleptic indicators, meat products did not differ from healthy ones, and only liver damage was noted. The liver is filled with blood, and the edges are dulled, the gallbladder is enlarged and filled with dark green bile, mucus and helminths. It was established that the liver is contaminated with microorganisms of the Enterobacteriaceae family, which were found on the 8th day of storage. Storage of the liver at a temperature of 0 °C for a day in samples No. 1, 4, 5 revealed bacteria of the Escherichia coli group, which increased by 27.7 % in the first sample; in the fourth for 10 days – by 16.1 %; 15 days in the 5th sample – by 10.8 % compared to the control, and also in the fifth sample Proteus vulgaris was isolated, which was 17.4 % and Staphylococcus aureus – 13.5 %. In the third tested sample, on the 8th day, bacteria of the Staphylococcus aureus species were found, which was 41.6 %. With a weak intensity of liver damage and contamination with the microflora of 31 CFU/cm3, slaughter products, namely the affected parts of the liver, are sent for disposal, and the non-affected parts of the liver and carcass are subjected to heat treatment and released for sale without restrictions. Following the current normative documents DSTU 4831:2006, DSTU 7444:2013, and DSTU ISO 6888-1:2003, with a high intensity of infestation, as well as in slaughter products, including liver, for the detection of more than 30 CFU/cm3, such a slaughter product is considered not fresh and subject to disposal. During the storage of beef liver for 15 days at a temperature of 0 °C, certain changes were detected, namely: the parenchyma of the organ is gray, has an unpleasant smell, the surface is covered with mucus, the consistency is flabby, the pit does not align when pressed with a finger

Histochemical method of diagnosis of mixed pasteurellosis and ascaridosis bird disease

For the first time was developed an effective and specific histochemical method for the diagnosis of mixed pasteurellosis and ascaridosis diseases of birds, which can be used in conjunction with bacteriological, serological and helminthocoproscopic studies, and in cases where it is impossible to diagnose serologic methods. The proposed method of histochemical diagnostics can be used for the detection of pasterelo-ascaridium carriers during mixed pasteurellosis and ascaridosis disease of birds. In the birds of the experimental group (clinically diseased bird), the pasteurial antigen in the histological preparations of the organs tissues after the histochemical study looked like local and diffuse clusters of oval and deep form of intense purple color and local clusters of oblong-shaped larvae with the presence of a head and a tail of blue and eggs of light purple color On histological sections of tissues and organs of the control group (clinically healthy bird), such changes were absent. For investigation of the organs of the experimental group (clinically diseased poultry), we have established the following areas of accumulation of pasteurial antigen and larvae and eggs of ascarids: in the heart – observed postponement of the antigen Pasteurella multocida in the form of strips throughout the area of the histological cut along the length of the muscle fibers and the loss of their delineation and fragmentation; in the liver – observed the diffuse location of the Pasteurella multocida antigen throughout the histological section in the lumen of the blood vessels and around them, the Disse spaces, between the hepatocytes; histochemically detected reduction of AsAT – by 84.3% and bilirubin – by 74.5%; in the muscular stomach – there was a diffuse postponement of the pasteurial antigen throughout the histologic section, but the largest accumulation was observed around the fibers of smooth muscle tissue of dark purple; in the spleen – the presence of Pasteurella multocida antigen throughout the histological section around lymphoid clusters, blood vessel walls, and intensive deposition of the antigen were observed around the splenic artery in the form of a deep, intense violet color; in the duodenum – observed diffuse deposition of the Pasteurella multocida antigen across the entire histologic area around the blood vessels, lymphatic follicles, villi of the mucous membrane, between the enterocytes, the mucosal muscle folds, the intramural ganglion nodules in the form of the deep form; Intestinal wall, blood vessels, licorice glands, larvae of ascarid species of Ascaridia galli species were observed blue, around granular grains around the dead larvae; Histochemically, decreased activity of alkaline phosphatase – by 70% and mucin – by 82.4%; in the kidneys – locally observed the presence of a Pasteurella multocida pasteur antigen around the intercellular veins, a capsule of the nephron, a circular oval-shaped distal tubule, a dark violet color; Histochemically, creatinine decreased by 45.4%

SLUDGE PARTICLE SEPAPATION EFFICIENCIES DURING SETTLER TANK RETRIEVAL INTO SCS-CON-230

The purpose of this document is to release, into the Hanford Document Control System, FA1/0991, Sludge Particle Separation Efficiencies for the Rectangular SCS-CON-230 Container, by M. Epstein and M. G. Plys, Fauske & Associates, LLC, June 2009. The Sludge Treatment Project (STP) will retrieve sludge from the 105-K West Integrated Water Treatment System (IWTS) Settler Tanks and transfer it to container SCS-CON-230 using the Settler Tank Retrieval System (STRS). The sludge will enter the container through two distributors. The container will have a filtration system that is designed to minimize the overflow of sludge fines from the container to the basin. FAI/09-91 was performed to quantify the effect of the STRS on sludge distribution inside of and overflow out of SCS-CON-230. Selected results of the analysis and a system description are discussed. The principal result of the analysis is that the STRS filtration system reduces the overflow of sludge from SCS-CON-230 to the basin by roughly a factor of 10. Some turbidity can be expected in the center bay where the container is located. The exact amount of overflow and subsequent turbidity is dependent on the density of the sludge (which will vary with location in the Settler Tanks) and the thermal gradient between the SCS-CON-230 and the basin. Attachment A presents the full analytical results. These results are applicable specifically to SCS-CON-230 and the STRS filtration system's expected operating duty cycles

Preliminary Safety Criteria for Organic Watch List Tanks at the Hanford Site

Condensed-phase, rapid reactions of organic salts with nitrates/nitrites in Hanford High Level Radioactive Waste single-shell tanks could lead to structural failure of the tanks resulting in significant releases of radionuclides and toxic materials. This report establishes appropriate preliminary safety criteria to ensure that tank wastes will be maintained safe. These criteria show that if actual dry wastes contain less than 1.2 MJ/kg of reactants reaction energy or less 4.5 wt % of total organic carbon, then the waste will be safe and will not propagate if ignited. Waste moisture helps to retard reactions; when waste moisture exceeds 20 wt %, rapid reactions are prevented, regardless of organic carbon concentrations. Aging and degradation of waste materials has been considered to predict the types and amounts to organic compounds present in the waste. Using measurements of 3 waste phases (liquid, salt cake, and sludge) obtained from tank waste samples analyzed in the laboratory, analysis of variance (ANOVA) models were used to estimate waste states for unmeasured tanks. The preliminary safety criteria are based upon calorimetry and propagation testing of likely organic compounds which represent actual tank wastes. These included sodium salts of citrate, formate, acetate and hydroxyethylethylenediaminetricetate (HEDTA). Hot cell tests of actual tank wastes are planned for the future to confirm propagation tests performed in the laboratory. The effects of draining liquids from the tanks which would remove liquids and moisture were considered because reactive waste which is too dry may propagate. Evaporation effects which could remove moisture from the tanks were also calculated. The various ways that the waste could be heated or ignited by equipment failures or tank operations activities were considered and appropriate monitoring and controls were recommended

Results and analysis of earth tide observations with the borehole tiltmeter in Poltava

Results of harmonic analysis of eight years earth tides observations with the borehole tiltmeter of the Poltava Gravimetric Observatory are presented. Hight-precision parameters of the main tidal waves and Love´s numbers h and k which practically coincide with similar data from  tiltmetric and gravimetric observations in 25 stations of Ukraine are received. The azimuthal inequality of a factor γ in the NS and EW directions isn't revealed. Resonant influence of the liquid core of Earth coincides with calculated theoretically

Safety insights from forensics evaluations at Daiichi

Although it is clear that the accident signatures from each affected unit at the Fukushima Daiichi Nuclear Power Station [Daiichi] differ, much is not known about the end-state of core materials within these units. Some of this uncertainty can be attributed to a lack of information related to cooling system operation and cooling water injection. There is also uncertainty in our understanding of phenomena affecting: a) in-vessel core damage progression during severe accidents in boiling water reactors (BWRs), and b) accident progression after vessel failure (ex-vessel progression) for BWRs and Pressurized Water Reactors (PWRs). These uncertainties arise due to limited full scale prototypic data. Similar to what occurred after the accident at Three Mile Island Unit 2, these Daiichi units offer the international community a means to reduce such uncertainties by obtaining prototypic data from multiple full-scale BWR severe accidents. Information obtained from Daiichi is required to inform Decontamination and Decommissioning activities, improving the ability of the Tokyo Electric Power Company (TEPCO) to characterize potential hazards and to ensure the safety of workers involved with cleanup activities. This paper reports initial results from the US Forensics Effort to utilize examination information obtained by TEPCO to enhance the safety of existing and future nuclear power plant designs. In this paper, three examples are presented in which examination information, such as visual images, dose surveys, sample evaluations, and muon tomography examinations, along with data from plant instrumentation, are used to obtain significant safety insights in the areas of component performance, fission product release and transport, debris end-state location, and combustible gas generation and transport. In addition to reducing uncertainties related to severe accident modeling progression, these insights confirm actions, such as the importance of water addition and containment venting, that are emphasized in updated guidance for severe accident prevention, mitigation, and emergency planning