Antioxidative activity of HDL particles from CETP-deficient (n = 9) and family control (n = 9) subjects.

<p>Reference LDL (10 mg TC/dl) was incubated at 37°C in PBS in the presence of AAPH (1 mmol/l). Small, dense HDL3b (A), HDL3c (B) or total HDL (C) particles were added to LDL directly before oxidation at 10 mg total mass/dl (A, B) or 40 mg total mass/dl (C). Accumulation of conjugated dienes was continuously measured as the increment in absorbance at 234 nm; two characteristic phases were identified, the lag phase and the propagation phase. To characterise the oxidation kinetics, oxidation rate within the propagation phase and duration of this phase were calculated for each absorbance curve.</p

Plasma levels of HDL particle subpopulations.

<p>Total lipoprotein mass was calculated as the sum of the mass of total protein, CE, FC, PL and TG for each HDL subpopulation isolated from CETP-deficient subjects (n = 9) and from family control subjects (n = 9).</p

Biological and clinical characteristics of subjects.

***<p>p<0.001, *p<0.05 vs controls. Numbers in parentheses denote % differences vs. controls.</p