ACYL-ACYL CARRIER PROTEIN DESATURASE2 and 3 are responsible for making omega-7 fatty acids in the Arabidopsis aleurone

Omega-7 monounsaturated fatty acids (ω-7s) are specifically enriched in the aleurone of Arabidopsis (Arabidopsis thaliana) seeds. We found significant natural variation in seed ω-7 content and used a Multiparent Advanced Generation Inter-Cross population to fine-map a major quantitative trait loci to a region containing ACYL-ACYL CARRIER PROTEIN DESATURASE1 (AAD1) and AAD3. We found that AAD3 expression is localized to the aleurone where mutants show an approximately 50% reduction in ω-7 content. By contrast, AAD1 is localized to the embryo where mutants show a small reduction in ω-9 content. Enzymatic analysis has previously shown that AAD family members possess both stearoyl- and palmitoyl-ACP Δ9 desaturase activity, including the predominant isoform SUPPRESSOR OF SALICYLIC ACID INSENSITIVE2. However, aad3 ssi2 aleurone contained the same amount of ω-7s as aad3. Within the AAD family, AAD3 shares the highest degree of sequence similarity with AAD2 and AAD4. Mutant analysis showed that AAD2 also contributes to ω-7 production in the aleurone, and aad3 aad2 exhibits an approximately 85% reduction in ω-7s. Mutant analysis also showed that FATTY ACID ELONGASE1 is required for the production of very long chain ω-7s in the aleurone. Together, these data provide genetic evidence that the ω-7 pathway proceeds via Δ9 desaturation of palmitoyl-ACP followed by elongation of the product. Interestingly, significant variation was also identified in the ω-7 content of Brassica napus aleurone, with the highest level detected being approximately 47% of total fatty acids

Halogen (F, Cl, Br, and I) Devolatilization During Prograde Subduction: Insights From Western Alps Ophiolites

In order to examine the progressive chemical evolution of halogens (F, Cl, Br, I) in altered ocean crust (AOC) during prograde subduction, this study compares bulk and in situ halogen concentrations in mafic samples from three petrogenetically related exhumed terrains in the Western Alps (the Chenaillet ophiolite, the Queyras ophiolites of the Schistes Lustrés, and the Monviso ophiolite). Samples from the Chenaillet ophiolite represent oceanic crust unaffected by metamorphic halogen loss and define a protolith halogen content (122 μg/g F, 29 μg/g Cl, 82 ng/g Br, and 98 ng/g I). Samples from the Queyras ophiolites experienced blueschist facies conditions, undergoing recrystallization and halogen loss (74 μg/g F, 19 μg/g Cl, 70 ng/g Br, and 63 ng/g I). Eclogite facies samples from the Monviso meta-ophiolite exhibit markedly reduced Cl (8 μg/g Cl) and Br (42 ng/g Br) contents relative to samples from Chenaillet and Queyras. Using electron probe microanalysis (EPMA), F and Cl host minerals (e.g., amphibole, chlorite, epidote) are identified and characterized in selected samples, showing a broad distribution of F and Cl, lending support to the view that halogen devolatilization in the subducting slab occurs continuously and is not dependent on the breakdown of a particular phase. In situ Cl concentrations decrease significantly between sub-greenschist and blueschist assemblages. Fluorine is retained within subducting AOC and is decoupled from the heavy halogens (Cl, Br, I), which undergo continuous devolatilization during prograde metamorphism

Dissipation and Ultrastrong Coupling in Circuit QED

Cavity and circuit QED study light-matter interaction at its most fundamental level. Yet, this interaction is most often neglected when considering the coupling of this system with an environment. In this paper, we show how this simplification, which leads to the standard quantum optics master equation, is at the root of unphysical effects. Including qubit relaxation and dephasing, and cavity relaxation, we derive a master equation that takes into account the qubit-resonator coupling. Special attention is given to the ultrastrong coupling regime, where the failure of the quantum optical master equation is manifest. In this situation, our model predicts an asymmetry in the vacuum Rabi splitting that could be used to probe dephasing noise at unexplored frequencies. We also show how fluctuations in the qubit frequency can cause sideband transitions, squeezing, and Casimir-like photon generation.Comment: 16 pages, 6 figure

Fluid pressure evolution in overpressured limestone reservoir at basin-scale: example of the Bighorn Basin (Wyoming, USA) and lessons from comparison with other reservoirs

Nicolas Beaudoin, Olivier Lacombe, Nicolas Bellahsen, Khalid Amrouch, and Jean-Marc Danie

Magnetothermodynamics of BPS baby skyrmions

The magnetothermodynamics of skyrmion type matter described by the gauged BPS baby Skyrme model at zero temperature is investigated. We prove that the BPS property of the model is preserved also for boundary conditions corresponding to an asymptotically constant magnetic field. The BPS bound and the corresponding BPS equations saturating the bound are found. Further, we show that one may introduce pressure in the gauged model by a redefinition of the superpotential. Interestingly, this is related to non-extremal type solutions in the so-called fake supersymmetry method. Finally, we compute the equation of state of magnetized BSP baby skyrmions inserted into an external constant magnetic field $H$ and under external pressure $P$, i.e., $V=V(P,H)$, where $V$ is the "volume" (area) occupied by the skyrmions. We show that the BPS baby skyrmions form a ferromagnetic medium.Comment: Latex, 39 pages, 14 figures. v2: New results and references added, physical interpretation partly change

Ultrafast x‐ray sources@f|

Time‐resolved spectroscopy (with a 2 psec temporal resolution) of plasmas produced by the interaction between solid targets and a high contrast subpicosecond table top terawatt (T3) laser at 1016 W/cm2, is used to study the basic processes which control the x‐ray pulse duration. Short x‐ray pulses have been obtained by spectral selection or by plasma gradient scalelength control. Time‐dependent calculations of the atomic physics [Phys. Fluids B 4, 2007, 1992] coupled to a Fokker–Planck code [Phys. Rev. Lett. 53, 1461, 1984] indicate that it is essential to take into account the non‐Maxwellian character of the electron distribution for a quantitative analysis of the experimental results.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/70417/2/PFBPEI-5-7-2676-1.pd

Transfer of MicroRNAs by Embryonic Stem Cell Microvesicles

Microvesicles are plasma membrane-derived vesicles released into the extracellular environment by a variety of cell types. Originally characterized from platelets, microvesicles are a normal constituent of human plasma, where they play an important role in maintaining hematostasis. Microvesicles have been shown to transfer proteins and RNA from cell to cell and they are also believed to play a role in intercellular communication. We characterized the RNA and protein content of embryonic stem cell microvesicles and show that they can be engineered to carry exogenously expressed mRNA and protein such as green fluorescent protein (GFP). We demonstrate that these engineered microvesicles dock and fuse with other embryonic stem cells, transferring their GFP. Additionally, we show that embryonic stem cells microvesicles contain abundant microRNA and that they can transfer a subset of microRNAs to mouse embryonic fibroblasts in vitro. Since microRNAs are short (21–24 nt), naturally occurring RNAs that regulate protein translation, our findings open up the intriguing possibility that stem cells can alter the expression of genes in neighboring cells by transferring microRNAs contained in microvesicles. Embryonic stem cell microvesicles may be useful therapeutic tools for transferring mRNA, microRNAs, protein, and siRNA to cells and may be important mediators of signaling within stem cell niches

Alcohol Facilitates CD1d Loading, Subsequent Activation of NKT Cells, and Reduces the Incidence of Diabetes in NOD Mice

Background: Ethanol ('alcohol') is a partly hydrophobic detergent that may affect the accessibility of glycolipids thereby influencing immunological effects of these molecules. Methods: The study included cellular in vitro tests using α-galactosylceramide (αGalCer), and in vivo NOD mice experiments detecting diabetes incidence and performing behavioural and bacterial analyses. Results: Alcohol in concentrations from 0.6% to 2.5% increased IL-2 production from NKT cells stimulated with αGalCer by 60% (p<0.05). CD1d expressed on HeLa cells contained significantly increasing amounts of αGalCer with increasing concentrations of alcohol, suggesting that alcohol facilitated the passive loading of αGalCer to CD1d. NOD mice were found to tolerate 5% ethanol in their drinking water without signs of impairment in liver function. Giving this treatment, the diabetes incidence declined significantly. Higher numbers of CD3+CD49b+ NKT cells were found in spleen and liver of the alcohol treated compared to the control mice (p<0.05), whereas the amount of CD4+Foxp3+ regulator T cells did not differ. Increased concentrations of IFN-γ were detected in 24-hour blood samples of alcohol treated mice. Behavioural studies showed no change in attitude of the ethanol-consuming mice, and bacterial composition of caecum samples was not affected by alcohol, disqualifying these as protective mechanisms. Conclusion: Alcohol facilitates the uptake of glycolipids and the stimulation of NKT cells, which are known to counteract Type 1 diabetes development. We propose that this is the acting mechanism by which treatment with alcohol reduces the incidence of diabetes in NOD mice. This is corroborated by epidemiology showing beneficial effect of alcohol to reduce the severity of atherosclerosis and related diseases

Construction and test of a fine-grained liquid argon preshower prototype

A separate liquid argon preshower detector consisting of two layers featuring a fine granularity of 2.5~10$^{\mathrm{-3}}$ was studied by the RD3 collaboration. A prototype covering approximately 0.8 in pseudo-rapidity and 9 degrees in azimuth was built and tested at CERN in July 94. CMOS and GaAs VLSI preamplifiers were designed and tested for this occasion. The combined response of this detector and an accordion electromagnetic calorimeter prototype to muons, electrons and photons is presented. For minimum ionizing tracks a signal-to-noise ratio of 4.5 per preshower layer was measured. Above 150~GeV the space resolution for electrons is better than 250~$\mu$m in both directions. The precision on the electromagnetic shower direction, determined together with the calorimeter, is better than 4 mrad above 50~GeV. It is concluded that the preshower detector would adequately fulfil its role for future operation at CERN Large Hadron Collider