Additively manufacturable micro-mechanical logic gates.

Early examples of computers were almost exclusively based on mechanical devices. Although electronic computers became dominant in the past 60 years, recent advancements in three-dimensional micro-additive manufacturing technology provide new fabrication techniques for complex microstructures which have rekindled research interest in mechanical computations. Here we propose a new digital mechanical computation approach based on additively-manufacturable micro-mechanical logic gates. The proposed mechanical logic gates (i.e., NOT, AND, OR, NAND, and NOR gates) utilize multi-stable micro-flexures that buckle to perform Boolean computations based purely on mechanical forces and displacements with no electronic components. A key benefit of the proposed approach is that such systems can be additively fabricated as embedded parts of microarchitected metamaterials that are capable of interacting mechanically with their surrounding environment while processing and storing digital data internally without requiring electric power

One-step volumetric additive manufacturing of complex polymer structures.

Two limitations of additive manufacturing methods that arise from layer-based fabrication are slow speed and geometric constraints (which include poor surface quality). Both limitations are overcome in the work reported here, introducing a new volumetric additive fabrication paradigm that produces photopolymer structures with complex nonperiodic three-dimensional geometries on a time scale of seconds. We implement this approach using holographic patterning of light fields, demonstrate the fabrication of a variety of structures, and study the properties of the light patterns and photosensitive resins required for this fabrication approach. The results indicate that low-absorbing resins containing ~0.1% photoinitiator, illuminated at modest powers (~10 to 100 mW), may be successfully used to build full structures in ~1 to 10 s

Sequestration of G3BP coupled with efficient translation inhibits stress granules in Semliki Forest virus infection

Dynamic, mRNA-containing stress granules (SGs) form in the cytoplasm of cells under environmental stresses, including viral infection. Many viruses appear to employ mechanisms to disrupt the formation of SGs on their mRNAs, suggesting that they represent a cellular defense against infection. Here, we report that early in Semliki Forest virus infection, the C-terminal domain of the viral nonstructural protein 3 (nsP3) forms a complex with Ras-GAP SH3-domain–binding protein (G3BP) and sequesters it into viral RNA replication complexes in a manner that inhibits the formation of SGs on viral mRNAs. A viral mutant carrying a C-terminal truncation of nsP3 induces more persistent SGs and is attenuated for propagation in cell culture. Of importance, we also show that the efficient translation of viral mRNAs containing a translation enhancer sequence also contributes to the disassembly of SGs in infected cells. Furthermore, we show that the nsP3/G3BP interaction also blocks SGs induced by other stresses than virus infection. This is one of few described viral mechanisms for SG disruption and underlines the role of SGs in antiviral defense

Improving sensitivity of a BEGe-based high-purity germanium spectrometer through pulse shape analysis

Abstract We performed Pulse Shape Analysis to separate single-scattered gamma energy deposition events from multiple-scattered photons in a high-sensitivity $$\gamma$$ γ -ray spectrometer. The spectrometer is based on a Broad Energy High Purity Germanium detector and the developed technique uses multivariate analysis by an application of the Multi-Layer Perceptron Neural Network. A very good separation of the single-site- and multi-site events was achieved leading to a significant reduction of the background level of the investigated spectrometer – the double escape peak, rich in single-site events, was reduced by 95%, while the full energy peaks lost at most 25% of their counts. The peak to Compton ratio, calculated for the 2614.5 keV gamma line from $$^{208}$$ 208 Tl, was improved by 114.3%

Evidence for electron transfer between graphene and non‐covalently bound π‐systems

Hybridizing graphene and molecules possess a high potential for developing materials for new applications. However, new methods to characterize such hybrids must be developed. Herein, the wet‐chemical non‐covalent functionalization of graphene with cationic π‐systems is presented and the interaction between graphene and the molecules is characterized in detail. A series of tricationic benzimidazolium salts with various steric demand and counterions was synthesized, characterized and used for the fabrication of graphene hybrids. Subsequently, the doping effects were studied. The molecules are adsorbed onto graphene and studied by Raman spectroscopy, XPS as well as ToF‐SIMS. The charged π‐systems show a p‐doping effect on the underlying graphene. Consequently, the tricationic molecules are reduced through a partial electron transfer process from graphene, a process which is accompanied by the loss of counterions. DFT calculations support this hypothesis and the strong p‐doping could be confirmed in fabricated monolayer graphene/hybrid FET devices. The results are the basis to develop sensor applications, which are based on analyte/molecule interactions and effects on doping

Design and fabrication of meso-scale flexural testing apparatus for evaluating aligned CNT composite flexures

Thesis (S.B.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2007.Includes bibliographical references (p. 58).The objective of this research is to explore the possibility of using aligned Carbon Nanotube (CNT) based composites in flexures by measuring the kinematics of a composite flexure. The first phase of the research, described in this thesis, is to design, fabricate and assemble a testing apparatus optimized for evaluating aligned CNT based composites. Using existing literature on composites and present limitations on their growth, functional requirements are set down for the testing apparatus. Several designs are qualitatively evaluated, leading to a near optimal design form. This chosen design is modeled as a spring-mass system, and the exact geometry needed to satisfy the functional requirements is determined. The design of the full apparatus is expanded to contain the necessary probes and actuators. The testing apparatus is fabricated using CNC machining, and assembled in a controlled environment to reduce thermal and mechanical error during operation. The system is calibrated and its resolution is found to be 0.021 N over a range of 28.5 N applied force and 1.5 pm over a range of 816 pm applied displacement. Several non-linearities are noted and corrected mathematically.by Robert M. Panas.S.B

Design and fabrication of a multipurpose compliant nanopositioning architecture

Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Mechanical Engineering, 2013.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student-submitted PDF version of thesis.Includes bibliographical references (p. 227-241).This research focused on generating the knowledge required to design and fabricate a high-speed application flexible, low average cost multipurpose compliant nanopositioner architecture with high performance integrated sensing. Customized nanopositioner designs can be created in ~~1 week, for 30x increase in sensing dynamic range over comparable state-of-the-art compliant nanopositioners. These improvements will remove one of the main hurdles to practical non-IC nanomanufacturing, which could enable advances in a range of fields including personalized medication, computing and data storage, and energy generation/storage through the manufacture of metamaterials. Advances were made in two avenues: flexibility and affordability. The fundamental advance in flexibility is the use of a new approach to modeling the nanopositioner and sensors as combined mechanical/electronic systems. This enabled the discovery of the operational regimes and design rules needed to maximize performance, making it possible to rapidly redesign nanopositioner architecture for varying functional requirements such as range, resolution and force. The fundamental advance to increase affordability is the invention of Non-Lithographically-Based Microfabrication (NLBM), a hybrid macro-/micro-fabrication process chain that can produce MEMS with integrated sensing in a flexible manner, at small volumes and with low per-device costs. This will allow for low-cost customizable nanopositioning architectures with integrated position sensing to be created for a range of micro-/nano- manufacturing and metrology applications. A Hexflex 6DOF nanopositioner with titanium flexures and integrated siliconpiezoresistive sensing was fabricated using NLBM. This device was designed with a metal mechanical structure in order to improve its robustness for general handling and operation. Single crystalline silicon piezoresistors were patterned from bulk silicon wafers and transferred to the mechanical structure via thin-film patterning and transfer. This work demonstrates that it is now feasible to design and create a customized positioner for each nanomanufacturing/metrology application. The Hexflex architecture can be significantly varied to adjust range, resolution, force scale, stiffness, and DOF all as needed. The NLBM process was shown to enable alignment of device components on the scale of 10's of microns. 150μm piezoresistor arm widths were demonstrated, with suggestions made for how to reach the expected lower bound of 25[mu]m. Flexures of 150[mu]m and 600[mu]m were demonstrated on 4 the mechanical structure, with a lower bound of ~~50[mu]m expected for the process. Electrical traces of 800[mu]m width were used to ensure low resistance, with a lower bound of ~~100[mu]m expected for the process. The integrated piezoresistive sensing was designed to have a gage factor of about 125, but was reduced to about 70 due to lower substrate temperatures during soldering, as predicted by design theory. The sensors were measured to have a full noise dynamic range of about 59dB over a 10kHz sensor bandwidth, limited by the Schottky barrier noise. Several simple methods are suggested for boosting the performance to ~~135dB over a 10kHz sensor bandwidth, about a <1Å resolution over the 200[mu]m range of the case study device. This sensor performance is generally in excess of presently available kHz-bandwidth analog-to-digital converters.by Robert M. Panas.Ph.D