Single-Photon Observables and Preparation Uncertainty Relations

We propose a procedure for defining all single-photon observables in terms of Positive-Operator Valued Measures (POVMs), in particular spin and position. We identify the suppression of $0$-helicity photon states as a projection from an extended Hilbert space onto the photon Hilbert space. We show that all single-photon observables are in general described by POVMs, obtained by applying this projection to opportune Projection-Valued Measures (PVMs), defined on the extended Hilbert space. The POVMs associated to momentum and helicity reduce to PVMs, unlike those associated to position and spin, this fact reflecting the intrinsic unsharpness of these observables. We finally extensively study the preparation uncertainty relations for position and momentum and the probability distribution of spin, exploring single photon Gaussian states for several choices of spin and polarization.Comment: 25 pages (7 Figures); revised and extended version; in submissio

Transient increases in intracellular calcium and reactive oxygen species levels in TCam-2 cells exposed to microgravity

The effects of microgravity on functions of the human body are well described, including alterations in the male and female reproductive systems. In the present study, TCam-2 cells, which are considered a good model of mitotically active male germ cells, were used to investigate intracellular signalling and cell metabolism during exposure to simulated microgravity, a condition that affects cell shape and cytoskeletal architecture. After a 24 hour exposure to simulated microgravity, TCam-2 cells showed 1) a decreased proliferation rate and a delay in cell cycle progression, 2) increased anaerobic metabolism accompanied by increased levels of intracellular Ca(2+), reactive oxygen species and superoxide anion and modifications in mitochondrial morphology. Interestingly, all these events were transient and were no longer evident after 48 hours of exposure. The presence of antioxidants prevented not only the effects described above but also the modifications in cytoskeletal architecture and the activation of the autophagy process induced by simulated microgravity. In conclusion, in the TCam-2 cell model, simulated microgravity activated the oxidative machinery, triggering transient macroscopic cell events, such as a reduction in the proliferation rate, changes in cytoskeleton-driven shape and autophagy activation

COMPARISON OF LOW COST PHOTOGRAMMETRIC SURVEY WITH TLS AND LEICA PEGASUS BACKPACK 3D MODELS

This paper considers Leica backpack and photogrammetric surveys of a mediaeval bastion in Padua, Italy. Furhtermore, terrestrial laser scanning (TLS) survey is considered in order to provide a state of the art reconstruction of the bastion. Despite control points are typically used to avoid deformations in photogrammetric surveys and ensure correct scaling of the reconstruction, in this paper a different approach is considered: this work is part of a project aiming at the development of a system exploiting ultra-wide band (UWB) devices to provide correct scaling of the reconstruction. In particular, low cost Pozyx UWB devices are used to estimate camera positions during image acquisitions. Then, in order to obtain a metric reconstruction, scale factor in the photogrammetric survey is estimated by comparing camera positions obtained from UWB measurements with those obtained from photogrammetric reconstruction. Compared with the TLS survey, the considered photogrammetric model of the bastion results in a RMSE of 21.9cm, average error 13.4cm, and standard deviation 13.5cm. Excluding the final part of the bastion left wing, where the presence of several poles make reconstruction more difficult, (RMSE) fitting error is 17.3cm, average error 11.5cm, and standard deviation 9.5cm. Instead, comparison of Leica backpack and TLS surveys leads to an average error of 4.7cm and standard deviation 0.6cm (4.2 cm and 0.3 cm, respectively, by excluding the final part of the left wing)

Learning activation functions from data using cubic spline interpolation

Neural networks require a careful design in order to perform properly on a given task. In particular, selecting a good activation function (possibly in a data-dependent fashion) is a crucial step, which remains an open problem in the research community. Despite a large amount of investigations, most current implementations simply select one fixed function from a small set of candidates, which is not adapted during training, and is shared among all neurons throughout the different layers. However, neither two of these assumptions can be supposed optimal in practice. In this paper, we present a principled way to have data-dependent adaptation of the activation functions, which is performed independently for each neuron. This is achieved by leveraging over past and present advances on cubic spline interpolation, allowing for local adaptation of the functions around their regions of use. The resulting algorithm is relatively cheap to implement, and overfitting is counterbalanced by the inclusion of a novel damping criterion, which penalizes unwanted oscillations from a predefined shape. Experimental results validate the proposal over two well-known benchmarks.Comment: Submitted to the 27th Italian Workshop on Neural Networks (WIRN 2017

Altered Kv2.1 functioning promotes increased excitability in hippocampal neurons of an Alzheimer's disease mouse model.

Altered neuronal excitability is emerging as an important feature in Alzheimer's disease (AD). Kv2.1 potassium channels are important modulators of neuronal excitability and synaptic activity. We investigated Kv2.1 currents and its relation to the intrinsic synaptic activity of hippocampal neurons from 3xTg-AD (triple transgenic mouse model of Alzheimer's disease) mice, a widely employed preclinical AD model. Synaptic activity was also investigated by analyzing spontaneous [Ca(2+)]i spikes. Compared with wild-type (Non-Tg (non-transgenic mouse model)) cultures, 3xTg-AD neurons showed enhanced spike frequency and decreased intensity. Compared with Non-Tg cultures, 3xTg-AD hippocampal neurons revealed reduced Kv2.1-dependent Ik current densities as well as normalized conductances. 3xTg-AD cultures also exhibited an overall decrease in the number of functional Kv2.1 channels. Immunofluorescence assay revealed an increase in Kv2.1 channel oligomerization, a condition associated with blockade of channel function. In Non-Tg neurons, pharmacological blockade of Kv2.1 channels reproduced the altered pattern found in the 3xTg-AD cultures. Moreover, compared with untreated sister cultures, pharmacological inhibition of Kv2.1 in 3xTg-AD neurons did not produce any significant modification in Ik current densities. Reactive oxygen species (ROS) promote Kv2.1 oligomerization, thereby acting as negative modulator of the channel activity. Glutamate receptor activation produced higher ROS levels in hippocampal 3xTg-AD cultures compared with Non-Tg neurons. Antioxidant treatment with N-Acetyl-Cysteine was found to rescue Kv2.1-dependent currents and decreased spontaneous hyperexcitability in 3xTg-AD neurons. Analogous results regarding spontaneous synaptic activity were observed in neuronal cultures treated with the antioxidant 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox). Our study indicates that AD-related mutations may promote enhanced ROS generation, oxidative-dependent oligomerization, and loss of function of Kv2.1 channels. These processes can be part on the increased neuronal excitability of these neurons. These steps may set a deleterious vicious circle that eventually helps to promote excitotoxic damage found in the AD brain

GRB 970228 Revisited: Evidence for a Supernova in the Light Curve and La te Spectral Energy Distribution of the Afterglow

At the time of its discovery, the optical and X-ray afterglow of GRB 970228 appeared to be a ringing endorsement of the previously untried relativistic fireball model of gamma-ray burst (GRB) afterglows, but now that nearly a dozen optical afterglows to GRBs have been observed, the wavering light curve and reddening spectrum of this afterglow make it perhaps the most difficult of the observed afterglows to reconcile with the fireball model. In this Letter, we argue that this afterglow's unusual temporal and spectral properties can be attributed to a supernova that overtook the light curve nearly two weeks after the GRB. This is the strongest case yet for a GRB/supernova connection. It strengthens the case that a supernova also dominated the late afterglow of GRB 980326, and the case that GRB 980425 is related to SN 1998bw.Comment: Accepted to The Astrophysical Journal (Letters), 14 pages, LaTe

Differences in the activity and distribution of peroxidase from three different portions of germinative Brassica oleracea seeds.

Peroxidase (POD, EC 1.11.1.7) activity, cellular localization and isozyme patterns were investigated in the seed integument, cotyledon and embryo axis of Brassica oleracea cv. Cappuccio during pregermination and seedling growth. Seeds started to germinate after 24 h of imbibition. POD activity was localized in the pigmented layer of the integument and in procambial strands of the cotyledon and embryo axis in the first 24 h of imbibition. It was localized in the integumental cells of palisade, pigmented and aleurone layers and in epidermal, meristematic, procambial cells and xylem elements of the root and hypocotyl after 48 h of imbibition. POD activity increased during germination and early seedling growth: in the integument, it reached a maximum value after 72 h of imbibition, in the embryo axis and cotyledons, it increased up to 144 h of imbibition. The increase in peroxidase activity was accompanied by the appearance of new isozymes correlated with the development of seedling tissues. The isozyme profile was characterized by nine peroxidases: isoperoxidase of 50 kDa peculiar to integuments, that of 150 kDa to cotyledons and that of 82 kDa to the embryo axis. During pregerminative phase isozymes of 84 kDa were detected in the integument and cotyledons, of 48.5 kDa in the embryo axis. After germination, peroxidase activity and the complexity of the isozyme pattern increased, suggesting that they play a relevant role after rupture of the integument