452 research outputs found
Physicochemical and Antibacterial Characterisation of a Novel Fluorapatite Coating
Peri-implantitis remains the major impediment to the long-term use of dental
implants. With increasing concern over growing antibiotic resistance there is
considerable interest in the preparation of antimicrobial dental implant coatings that
also induce osseointegration. One such potential coating material is fluorapatite
(FA). The aim of this study was to relate the antibacterial effectiveness of FA
coatings against pathogens implicated in peri-implantitis to the physicochemical
properties of the coating. Ordered and disordered FA coatings were produced on the
under and upper surface of stainless steel (SS) discs respectively, using a
hydrothermal method. Surface charge, surface roughness, wettability and fluoride
release were measured for each coating. Surface chemistry was assessed by X-ray
photoelectron spectroscopy and FA crystallinity by X-ray diffraction. Antibacterial
activity against periodontopathogens was assessed in vitro using viable counts,
confocal and scanning electron (SEM) microscopies. SEM showed that the
hydrothermal method produced FA coatings predominately aligned perpendicular to
the SS substrate or disordered FA coatings consisting of randomly aligned rod-like
crystals. Both FA coatings significantly reduced the growth of all the examined
bacterial strains in comparison to the control. The FA coatings, and especially the
disordered ones, presented significantly lower charge, higher roughness and area
when compared to the control, enhancing bacteria–material interactions and
therefore bacterial deactivation by fluoride ions. The ordered FA layer reduced not
only bacterial viability but adhesion too. Ordered FA crystals produced as a potential
novel implant coating showed significant antibacterial activity against bacteria
implicated in peri-implantitis which could be explained by a detailed understanding of
their physicochemical properties
Identification and categorisation of safety issues for ESNII reactor concepts. Part I: Common phenomena related to materials
International audience; With the aim to develop a joint proposal for a harmonised European methodology for safety assessment of advanced reactors with fast neutron spectrum, SARGEN-IV (Safety Assessment for Reactors of Gen IV) Euratom coordination action project gathered together twenty-two partners' safety experts from twelve EU Member States. The group consisted of eight European Technical Safety Organisations involved in the European Technical Safety Organisation Network (ETSON), European Commission's Joint Research Centre (JRC), system designers, industrial vendors as well as research and development (RandD) organisations. To support the methodology development, key safety features of four fast neutron spectrum reactor concepts considered in Deployment Strategy of the Sustainable Nuclear Energy Technology Platform (SNETP) were reviewed. In particular, outcomes from running European Sustainable Nuclear Industrial Initiative (ESNII) system projects and related Euratom collaborative projects for Sodium-cooled Fast Reactors, Lead-cooled Fast Reactors, Gas-cooled Fast Reactors, and the lead-bismuth eutectic cooled Fast Spectrum Transmutation Experimental Facility were gathered and critically assessed. To allow a consistent build-up of safety architecture for the ESNII reactor concepts, the safety issues were further categorised to identify common phenomena related to materials. Outcomes of the present work also provided guidance for the identification and prioritisation of further RandD needs respective to the identified safety issues. © 2015 The Authors. Published by Elsevier Ltd. This is an open access article under the CC BY-NC-NDlicense
U and Th content in the Central Apennines continental crust: a contribution to the determination of the geo-neutrinos flux at LNGS
The regional contribution to the geo-neutrino signal at Gran Sasso National
Laboratory (LNGS) was determined based on a detailed geological, geochemical
and geophysical study of the region. U and Th abundances of more than 50
samples representative of the main lithotypes belonging to the Mesozoic and
Cenozoic sedimentary cover were analyzed. Sedimentary rocks were grouped into
four main "Reservoirs" based on similar paleogeographic conditions and
mineralogy. Basement rocks do not outcrop in the area. Thus U and Th in the
Upper and Lower Crust of Valsugana and Ivrea-Verbano areas were analyzed. Based
on geological and geophysical properties, relative abundances of the various
reservoirs were calculated and used to obtain the weighted U and Th abundances
for each of the three geological layers (Sedimentary Cover, Upper and Lower
Crust). Using the available seismic profile as well as the stratigraphic
records from a number of exploration wells, a 3D modelling was developed over
an area of 2^{\circ}x2^{\circ} down to the Moho depth, for a total volume of
about 1.2x10^6 km^3. This model allowed us to determine the volume of the
various geological layers and eventually integrate the Th and U contents of the
whole crust beneath LNGS. On this base the local contribution to the
geo-neutrino flux (S) was calculated and added to the contribution given by the
rest of the world, yielding a Refined Reference Model prediction for the
geo-neutrino signal in the Borexino detector at LNGS: S(U) = (28.7 \pm 3.9) TNU
and S(Th) = (7.5 \pm 1.0) TNU. An excess over the total flux of about 4 TNU was
previously obtained by Mantovani et al. (2004) who calculated, based on general
worldwide assumptions, a signal of 40.5 TNU. The considerable thickness of the
sedimentary rocks, almost predominantly represented by U- and Th- poor
carbonatic rocks in the area near LNGS, is responsible for this difference.Comment: 45 pages, 5 figures, 12 tables; accepted for publication in GC
Комплексна система захисту інформації відділу розробки сільськогосподарської техніки ТОВ «Промислова Група «Корсунь»
Брижата Н. Ю. Комплексна система захисту інформації відділу розробки сільськогосподарської техніки ТОВ «Промислова Група «Корсунь» : кваліфікаційна робота бакалавра / Брижата Наталія Юріївна. – Дніпро, 2020. – 92 с.В кваліфікаційній роботі виконана розробка КСЗІ. Наведено загальні відомості про об’єкт інформаційної діяльності. Проведено обстеження об’єкту інформаційної діяльності, категоріювання інформаційно-телекомунікаційної системи, обрано профіль захищеності. Визначені основні загрози
Pharmacokinetic/pharmacodynamic modelling approaches in paediatric infectious diseases and immunology.
Pharmacokinetic/pharmacodynamic (PKPD) modelling is used to describe and quantify dose-concentration-effect relationships. Within paediatric studies in infectious diseases and immunology these methods are often applied to developing guidance on appropriate dosing. In this paper, an introduction to the field of PKPD modelling is given, followed by a review of the PKPD studies that have been undertaken in paediatric infectious diseases and immunology. The main focus is on identifying the methodological approaches used to define the PKPD relationship in these studies. The major findings were that most studies of infectious diseases have developed a PK model and then used simulations to define a dose recommendation based on a pre-defined PD target, which may have been defined in adults or in vitro. For immunological studies much of the modelling has focused on either PK or PD, and since multiple drugs are usually used, delineating the relative contributions of each is challenging. The use of dynamical modelling of in vitro antibacterial studies, and paediatric HIV mechanistic PD models linked with the PK of all drugs, are emerging methods that should enhance PKPD-based recommendations in the future
Computer-aided assessment of diagnostic images for epidemiological research
<p>Abstract</p> <p>Background</p> <p>Diagnostic images are often assessed for clinical outcomes using subjective methods, which are limited by the skill of the reviewer. Computer-aided diagnosis (CAD) algorithms that assist reviewers in their decisions concerning outcomes have been developed to increase sensitivity and specificity in the clinical setting. However, these systems have not been well utilized in research settings to improve the measurement of clinical endpoints. Reductions in bias through their use could have important implications for etiologic research.</p> <p>Methods</p> <p>Using the example of cortical cataract detection, we developed an algorithm for assisting a reviewer in evaluating digital images for the presence and severity of lesions. Available image processing and statistical methods that were easily implementable were used as the basis for the CAD algorithm. The performance of the system was compared to the subjective assessment of five reviewers using 60 simulated images. Cortical cataract severity scores from 0 to 16 were assigned to the images by the reviewers and the CAD system, with each image assessed twice to obtain a measure of variability. Image characteristics that affected reviewer bias were also assessed by systematically varying the appearance of the simulated images.</p> <p>Results</p> <p>The algorithm yielded severity scores with smaller bias on images where cataract severity was mild to moderate (approximately ≤ 6/16<sup><it>ths</it></sup>). On high severity images, the bias of the CAD system exceeded that of the reviewers. The variability of the CAD system was zero on repeated images but ranged from 0.48 to 1.22 for the reviewers. The direction and magnitude of the bias exhibited by the reviewers was a function of the number of cataract opacities, the shape and the contrast of the lesions in the simulated images.</p> <p>Conclusion</p> <p>CAD systems are feasible to implement with available software and can be valuable when medical images contain exposure or outcome information for etiologic research. Our results indicate that such systems have the potential to decrease bias and discriminate very small changes in disease severity. Simulated images are a tool that can be used to assess performance of a CAD system when a gold standard is not available.</p
Self-reported dental hygiene, obesity, and systemic inflammation in a pediatric rural community cohort
Background
A growing body of epidemiologic evidence links oral health, obesity, and cardiovascular health, though few studies have reported on these relationships in children. While underlying mechanisms are unclear, adult studies have suggested sub-acute systemic inflammation, also implicated in the etiology of both obesity and cardiovascular disease. This study investigated associations between self-reported dental hygiene, obesity, and systemic inflammation in children. Methods
128 children \u3c 19 years of age from rural counties in West Virginia participated in a community-based health screening that included anthropometric assessments, blood collection, and a questionnaire about dental hygiene and self-assessed oral health. Results
Participants ranged from 3.0-18.7 years. Univariate analysis demonstrated an association between parent-reported dental hygiene, including frequency of preventive dental care and parent-assessed overall dental health, and markers of systemic inflammation but not obesity. In multivariable regression, parent-assessed overall dental health and obesity were independent predictors of systemic inflammation, after adjustment for age, gender, and parent education. Conclusions
This is the first known study of the association between dental hygiene, obesity, and systemic inflammation in children. These results highlight the importance of preventive dental care in overall, systemic health in children and are consistent with previous reports in adults
Therapeutic Potential and Challenges of Targeting Receptor Tyrosine Kinase ROR1 with Monoclonal Antibodies in B-Cell Malignancies
Based on its selective cell surface expression in chronic lymphocytic leukemia (CLL) and mantle cell lymphoma (MCL), receptor tyrosine kinase ROR1 has recently emerged as a promising target for therapeutic monoclonal antibodies (mAbs). To further assess the suitability of ROR1 for targeted therapy of CLL and MCL, a panel of mAbs was generated and its therapeutic utility was investigated.A chimeric rabbit/human Fab library was generated from immunized rabbits and selected by phage display. Chimeric rabbit/human Fab and IgG1 were investigated for their capability to bind to human and mouse ROR1, to mediate antibody-dependent cellular cytotoxicity (ADCC), complement-dependent cytotoxicity (CDC), and internalization, and to agonize or antagonize apoptosis using primary CLL cells from untreated patients as well as MCL cell lines. A panel of mAbs demonstrated high affinity and specificity for a diverse set of epitopes that involve all three extracellular domains of ROR1, are accessible on the cell surface, and mediate internalization. The mAb with the highest affinity and slowest rate of internalization was found to be the only mAb that mediated significant, albeit weak, ADCC. None of the mAbs mediated CDC. Alone, they did not enhance or inhibit apoptosis.Owing to its relatively low cell surface density, ROR1 may be a preferred target for armed rather than naked mAbs. Provided is a panel of fully sequenced and thoroughly characterized anti-ROR1 mAbs suitable for conversion to antibody-drug conjugates, immunotoxins, chimeric antigen receptors, and other armed mAb entities for preclinical and clinical studies
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