Perfil sociodemográfico del Partido de Gral. Alvarado : Un aporte al análisis de la sustentabilidad ambiental

La presente ponencia forma parte de un proyecto de investigación mayor, el cual tiene como objetivo propiciar instancias diagnósticas socioterritoriales del Partido de Gral. Alvarado en perspectiva ambiental, como base para ulteriores estudios de sustentabilidad ambiental. El diagnóstico requiere la identificación y análisis de configuraciones y organizaciones socioterritoriales desde un análisis que plantee la relación demanda socioambiental, referida a la población y sus actividades con la oferta biofísica del medio natural y las tecnoestructuras. Esferas, entre las que se plantean desajustes desde tales relaciones, desde lo que se procurará explicar e interpretar las problemáticas socioambientales emergentes, enfatizando en la riqueza de aportes de los actores sociales mediante abordajes teórico-metodológicos ad hoc (Bryant y Bayley, 1997).Evento también conocido bajo el nombre de "II Jornadas Platenses de Geografía"Facultad de Humanidades y Ciencias de la Educació

Component-resolved diagnosis of wheat flour allergy in baker's asthma.

Baker's asthma is one of the most common types of occupational asthma and its prevalence is increasing in the last years. Diagnosis of occupational asthma is complex. The poor specificity of current diagnostic approaches may be associated with insufficient purity of wheat extracts or lack of inclusion of major allergens in them. In this work, we use microarray technology to characterize the allergenic profiles of baker's asthma patients from three regions in Spain and to analyze the influence of other environmental allergens on the sensitization pattern

NR2B receptor blockade inhibits pain-related sensitization of amygdala neurons

Pain-related sensitization and synaptic plasticity in the central nucleus of the amygdala (CeA) depend on the endogenous activation of NMDA receptors and phosphorylation of the NR1 subunit through a PKA-dependent mechanism. Functional NMDA receptors are heteromeric assemblies of NR1 with NR2A-D or NR3A, B subunits. NMDA receptors composed of NR1 and NR2B subunits have been implicated in neuroplasticity and are present in the CeA. Here we used a selective NR2B antagonist (Ro-256981) to determine the contribution of NR2B-containing NMDA receptors to pain-related sensitization of CeA neurons. Extracellular single-unit recordings were made from CeA neurons in anesthetized adult male rats before and during the development of an acute arthritis. Arthritis was induced in one knee joint by intraarticular injections of kaolin and carrageenan. Brief (15 s) mechanical stimuli of innocuous (100–500 g/30 mm2) and noxious (1000–2000 g/30 mm2) intensity were applied to the knee and other parts of the body. In agreement with our previous studies, all CeA neurons developed increased background and evoked activity after arthritis induction. Ro-256981 (1, 10 and 100 μM; 15 min each) was administered into the CeA by microdialysis 5–6 h postinduction of arthritis. Ro-256981 concentration-dependently decreased evoked responses, but not background activity. This pattern of effect is different from that of an NMDA receptor antagonist (AP5) in our previous studies. AP5 (100 μM – 5 mM) inhibited background activity and evoked responses. The differential effects of AP5 and Ro-256981 may suggest that NMDA receptors containing the NR2B subunit are important but not sole contributors to pain-related changes of CeA neurons

Risk of allergic reactions to wine, in milk, egg and fish-allergic patients

<p>Abstract</p> <p>Background</p> <p>European legislators and wine producers still debate on the requirement for labeling of wines fined with potentially allergenic food proteins (casein, egg white or fish-derived isinglass). We investigated whether wines fined with known concentrations of these proteins have the potential to provoke clinical allergic reactions in relevant patients.</p> <p>Methods</p> <p>In-house wines were produced for the study, fined with different concentrations of casein (n = 7), egg albumin (n = 1) and isinglass (n = 3). ELISA and PCR kits specific for the respective proteins were used to identify the fining agents. Skin prick tests and basophil activation tests were performed in patients with confirmed IgE-mediated relevant food allergies (n = 24). A wine consumption questionnaire and detailed history on possible reactions to wine was obtained in a multinational cohort of milk, egg or fish allergic patients (n = 53) and patients allergic to irrelevant foods as controls (n = 13).</p> <p>Results</p> <p>Fining agents were not detectable in wines with the available laboratory methods. Nevertheless, positive skin prick test reactions and basophil activation to the relevant wines were observed in the majority of patients with allergy to milk, egg or fish, correlating with the concentration of the fining agent. Among patients consuming wine, reported reactions were few and mild and similar with the ones reported from the control group.</p> <p>Conclusion</p> <p>Casein, isinglass or egg, remaining in traces in wine after fining, present a very low risk for the respective food allergic consumers. Physician and patient awareness campaigns may be more suitable than generalized labeling to address this issue, as the latter may have negative impact on both non-allergic and allergic consumers.</p

Administration of eCG is not recommended in time-AIprotocols for beef cattle in good body condition (póster)

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Ensayo de un sistema de indicadores sincrónico y diacrónico para la determinación de calidad ambiental en playas turísticas de la costa atlántica bonaerense

El subsistema natural litoral ha sido objeto de innumerables trabajos debido a la presión que la población puede generar en él, sin embargo la determinación cuantitativa de la calidad ambiental,en playas con uso turístico, es de algún modo, reciente. El objetivo del estudio es ensayar un sistema de indicadores, agrupados según un modelo de estado, presión y respuesta, para la determinación de la calidad ambiental en playas con uso turístico. Las mediciones se realizaron en diez playas, en los meses de verano, desde el 2009 al 2013. La calidad ambiental que afecta la función amenidad se determinó por una adaptación del modelo Presión, Estado y Respuesta (PER) complementada con análisis univariados y bivariados. Las relaciones significativas entre las variables consideradas muestran que el uso de la playa responde a cuestiones como la calidad de las arenas o la presencia de plataformas de erosión. Las playas con mayores presiones son las de Santa Teresita y Mar del Plata mientras que las de Villa Gesell y Miramar son las que presentan mejor Estado relativo por ser amplias, sin plataformas de erosión, con buena calidad microbiológica, y en general con baja turbidez de sus aguas,con ausencia de olores desagradables,entre otras características. Por otro lado las que presentan peor estado son las playas de Quequén y Santa Teresita por carecer de los atributos antes mencionados. Las mejores respuestas relativas se registraron en Villa Gesell y Miramar donde se verifica una gestión privada.Natural littoral subsystems have been the subject of countless research projects because of the pressure that people can generate onthem. However the quantitative determination of environmental quality, in beaches with tourist use, is of recent implementation. The aim of this study is to apply a system of indicators grouped according to a model state, pressure and response to the determination of environmental quality in beaches used for tourist purposes. Measurements were performed on ten beaches during summer months, from 2009 to 2013. The environmental quality that affects the amenity function was determined by an adaptation of Pressure, State and Response (PER) model, supplemented with univariate and bivariate analysis. Significant relationships between variables show that the use of the beach responds to the quality of the sand or the presence of erosion platforms. The beaches Santa Teresita and Mar del Plata show the highest pressures while Villa Gesell and Miramar are those with bestrelative state due to their amplitude, without erosion platforms, with good microbiological quality, and in general, with low turbidity in their waters, or absence of unpleasant odors, among other features. On the other hand those with worststate are Santa Teresitaand Quequén beaches because they lack of the above mentioned attributes. The best responses were recorded on Villa Gesell and Miramar where a private management is verified

Biofabrication of functional protein nanoparticles through simple His-tag engineering

Altres ajuts: Acord transformatiu CRUE-CSICWe have developed a simple, robust, and fully transversal approach for thea-la-cartefabrication of functional multimeric nanoparticles with potential biomedical applications, validated here by a set of diverse and unrelated polypeptides. The proposed concept is based on the controlled coordination between Znions and His residues in His-tagged proteins. This approach results in a spontaneous and reproducible protein assembly as nanoscale oligomers that keep the original functionalities of the protein building blocks. The assembly of these materials is not linked to particular polypeptide features, and it is based on an environmentally friendly and sustainable approach. The resulting nanoparticles, with dimensions ranging between 10 and 15 nm, are regular in size, are architecturally stable, are fully functional, and serve as intermediates in a more complex assembly process, resulting in the formation of microscale protein materials. Since most of the recombinant proteins produced by biochemical and biotechnological industries and intended for biomedical research are His-tagged, the green biofabrication procedure proposed here can be straightforwardly applied to a huge spectrum of protein species for their conversion into their respective nanostructured formats

Synaptic Maturation at Cortical Projections to the Lateral Amygdala in a Mouse Model of Rett Syndrome

Rett syndrome (RTT) is a neuro-developmental disorder caused by loss of function of Mecp2 - methyl-CpG-binding protein 2 - an epigenetic factor controlling DNA transcription. In mice, removal of Mecp2 in the forebrain recapitulates most of behavioral deficits found in global Mecp2 deficient mice, including amygdala-related hyper-anxiety and lack of social interaction, pointing a role of Mecp2 in emotional learning. Yet very little is known about the establishment and maintenance of synaptic function in the adult amygdala and the role of Mecp2 in these processes. Here, we performed a longitudinal examination of synaptic properties at excitatory projections to principal cells of the lateral nucleus of the amygdala (LA) in Mecp2 mutant mice and their wild-type littermates. We first show that during animal life, Cortico-LA projections switch from a tonic to a phasic mode, whereas Thalamo-LA synapses are phasic at all ages. In parallel, we observed a specific elimination of Cortico-LA synapses and a decrease in their ability of generating presynaptic long term potentiation. In absence of Mecp2, both synaptic maturation and synaptic elimination were exaggerated albeit still specific to cortical projections. Surprisingly, associative LTP was unaffected at Mecp2 deficient synapses suggesting that synaptic maintenance rather than activity-dependent synaptic learning may be causal in RTT physiopathology. Finally, because the timing of synaptic evolution was preserved, we propose that some of the developmental effects of Mecp2 may be exerted within an endogenous program and restricted to synapses which maturate during animal life

Genetic dissection of an amygdala microcircuit that gates conditioned fear

The role of different amygdala nuclei (neuroanatomical subdivisions) in processing Pavlovian conditioned fear has been studied extensively, but the function of the heterogeneous neuronal subtypes within these nuclei remains poorly understood. Here we use molecular genetic approaches to map the functional connectivity of a subpopulation of GABA-containing neurons, located in the lateral subdivision of the central amygdala (CEl), which express protein kinase C-δ (PKC-δ). Channelrhodopsin-2-assisted circuit mapping in amygdala slices and cell-specific viral tracing indicate that PKC-δ^+ neurons inhibit output neurons in the medial central amygdala (CEm), and also make reciprocal inhibitory synapses with PKC-δ^− neurons in CEl. Electrical silencing of PKC-δ^+ neurons in vivo suggests that they correspond to physiologically identified units that are inhibited by the conditioned stimulus, called Cel_(off) units. This correspondence, together with behavioural data, defines an inhibitory microcircuit in CEl that gates CEm output to control the level of conditioned freezing