Entomological characterization of malaria in northern Colombia through vector and parasite species identification, and analyses of spatial distribution and infection rates

Background: Malaria remains a worldwide public health concern and, in Colombia, despite the efforts to stop malaria transmission, the incidence of cases has increased over the last few years. In this context, it is necessary to evaluate vector diversity, infection rates, and spatial distribution, to better understand disease transmission dynamics. This information may contribute to the planning and development of vector control strategies. Results: A total of 778 Anopheles mosquitoes were collected in fifteen localities of Córdoba from August 2015 to October 2016. Six species were identified and overall, Anopheles albimanus was the most widespread and abundant species (83%). Other species of the Nyssorhynchus subgenus were collected, including Anopheles triannulatus (13%), Anopheles nuneztovari (1%), Anopheles argyritarsis (< 1%) and two species belonging to the Anopheles subgenus: Anopheles pseudopunctipennis (3%) and Anopheles neomaculipalpus (< 1%). Four species were found naturally infected with two Plasmodium species: Anopheles nuneztovari was detected naturally infected with Plasmodium falciparum and Anopheles pseudopunctipennis with Plasmodium vivax, whereas An. albimanus and An. triannulatus were found infected with both parasite species and confirmed by nested PCR. Conclusions: In general, the obtained results were contrasting with previous studies in terms of the most abundant and widespread collected species, and regarding infection rates, which were higher than those previously reported. A positive relationship between mosquito local abundance at the locality level and human infection at the municipality level was found. Mosquito local abundance and the number of houses with mosquitoes in each village are factors explaining malaria human cases in these villages. The obtained results suggest that other factors related to the apparent variation in malaria eco-epidemiology in northern Colombia, must be identified, to provide health authorities with better decision tools aiming to design control and prevention strategies

Effect of Electrolyte Balance in Low-Protein Diets on Broiler Performance and Tibial Dyschondroplasia Incidence

A proper dietary electrolyte balance (DEB) is essential to ensure an optimum acid-base equilibrium and broiler performance. In low-CP diets, this balance can be affected by reduction of soybean meal and inclusion of high levels of synthetic amino acids. Although, some studies have related low-protein diets supplemented with amino acids and DEB, these relations are not well explained, because some research demonstrates confusion about the deficiency and balance of nutrients. The objective of these experiments was to evaluate the DEB effects of diets with low levels of protein supplemented with amino acids on broiler performance and bone development. Results indicated that DEB and CP content influenced broiler chick performance in the starter and growing periods. There was no significant effect due to the interaction between DEB and CP content for tibial dyschondroplasia incidence (TD) or in bone breaking resistance during the growing period of either experiment. The incidence of TD was reduced with 253 mEq/kg DEB in the starter period

Chromomagnetism in nuclear matter

Quarks are color charged particles. Due to their motion there is a strong possibility of generation of color magnetic field. It is shown that however hadrons are color singlet particles they may have non-zero color magnetic moment. Due to this color magnetic moment hadrons can show color interaction. In this paper we have studied the chromomagnetic properties of nuclear matter.Comment: 6 pages, 1 figure, accepted for publication in Int. J. Theor. Phy

Seasonality, richness and prevalence of intestinal parasites of three neotropical primates (Alouatta seniculus, Ateles hybridus and Cebus versicolor) in a fragmented forest in Colombia

Studies on parasites infecting non-human primates are essential to better understand the potential threat to humans of zoonoses transmission, particularly under the current processes of pervasive land use change and biodiversity loss. The natural ecosystems in the Middle Magdalena river basin in Colombia have suffered a dramatic reduction and transformation into pastures and agroindustrial monocultures, threatening their biodiversity, and probably affecting the dynamics between parasites and their hosts, as well as altering the disease transmission cycles between wild populations and humans. This study evaluated seasonality, prevalence and richness of intestinal parasites in three species of neotropical primates: Cebus versicolor, Ateles hybridus and Alouatta seniculus, in a fragmented forest in the Middle Magdalena river valley, Colombia. One hundred and eighty five faecal samples were collected between 2010 and 2015. Direct faecal smears were performed with saline solution (0.85%) and iodine solution (1%), in order to identify larvae and eggs based on their morphology. A large proportion of the samples examined (72.9%) was positive for intestinal parasites; seven families of nematodes were identified: Trichuridae, Trichostrongylidae, Oxyuridae, Strongyloididae, Ancylostomatidae, Ascarididae and Gnathostomatidae, two of protozoans: Entamoebidae and Balantiididae, as well as some eggs of trematodes, cestodes and acanthocephalans. Additionally, DNA extraction and sequencing were conducted on 30 faecal samples in order to identify Giardia sp. and Blastocystis hominis, two parasite species also present in humans. Molecular testing for Giardia sp. was negative and Blastocystis hominis was identified in a single sample of Alouatta seniculus. No clear patterns were observed for prevalence of intestinal parasites according to the season; nonetheless, parasite species richness was higher during the dry season. This study builds on our current understanding of intestinal parasites infecting wild neotropical primates and provides novel information on the patterns of intestinal parasites in primate communities exposed to anthropogenic disturbance

Enzymes as Feed Additive to Aid in Responses Against Eimeria Species in Coccidia-Vaccinated Broilers Fed Corn-Soybean Meal Diets with Different Protein Levels

This research aimed to evaluate the effects of adding a combination of exogenous enzymes to starter diets varying in protein content and fed to broilers vaccinated at day of hatch with live oocysts and then challenged with mixed Eimeria spp. Five hundred four 1-d-old male Cobb-500 chickens were distributed in 72 cages. The design consisted of 12 treatments. Three anticoccidial control programs [ionophore (IO), coccidian vaccine (COV), and coccidia-vaccine + enzymes (COV + EC)] were evaluated under 3 CP levels (19, 21, and 23%), and 3 unmedicated-uninfected (UU) negative controls were included for each one of the protein levels. All chickens except those in unmedicated-uninfected negative controls were infected at 17 d of age with a mixed oral inoculum of Eimeria acervulina, Eimeria maxima, and Eimeria tenella. Live performance, lesion scores, oocyst counts, and samples for gut microflora profiles were evaluated 7 d postinfection. Ileal digestibility of amino acids (IDAA) was determined 8 d postinfection. Microbial communities (MC) were analyzed by G + C%, microbial numbers were counted by flow cytometry, and IgA concentrations were measured by ELISA. The lowest CP diets had poorer (P ≤ 0.001) BW gain and feed conversion ratio in the preinfection period. Coccidia-vaccinated broilers had lower performance than the ones fed ionophore diets during pre- and postchallenge periods. Intestinal lesion scores were affected (P ≤ 0.05) by anticoccidial control programs, but responses changed according to gut section. Feed additives or vaccination had no effect (P ≥ 0.05) on IDAA, and diets with 23% CP had the lowest (P ≤ 0.001) IDAA. Coccidial infection had no effect on MC numbers in the ileum but reduced MC numbers in ceca and suppressed ileal IgA production. The COV + EC treatment modulated MC during mixed coccidiosis infection but did not significantly improve chicken performance. Results indicated that feed enzymes may be used to modulate the gut microflora of cocci-vaccinated broiler chickens

Crescimento micelial in vitro de treze isolados de Corynespora cassiicola submetidos a diferentes temperaturas.

Corynespora cassiicola (Berk. & Curt.), fungo que causa a doença conhecida como mancha-alvo na cultura da soja ocorre em todo o Brasil. Considerando que os isolados de C. cassiicola apresentam variações nas características morfológicas, comportando-se de maneira distinta quanto ao crescimento micelial, o estudo do desenvolvimento deste fungo faz-se necessário para facilitar a multiplicação de inóculo em laboratório. Por este motivo, o objetivo foi avaliar o crescimento micelial in vitro de 13 isolados de C. cassiicola submetidos a diferentes temperaturas. Isolados da micoteca da Embrapa soja (307, 310, 311, 312, 313, 317, 318, 322, 605, 629, 646, 649 e 651) foram repicados em placas de Petri contendo BDA e incubados nas temperaturas de 18, 21, 24, 27 e 30 ± 2ºC, sendo a avaliação do crescimento micelial realizada por medições diárias do diâmetro das colônias. O delineamento experimental foi inteiramente casualizado em arranjo fatorial 13 x 5 (isolados x temperaturas) totalizando 65 tratamentos com cinco repetições. Com os dados obtidos calculou-se o índice de velocidade de crescimento micelial (IVCM) que foram submetidos à análise de variância, sendo as médias comparadas por meio do teste de Scott-Knott, ao nível de 5% de probabilidade. Verificou-se diferença estatística entre o crescimento micelial dos isolados, entre as temperaturas e também na interação isolado x temperatura. A maioria dos isolados apresentou maior IVCM quando incubados em temperaturas superiores a 24ºC, com exceção do isolado 307 que atingiu maior IVCM em temperaturas inferiores a 24ºC. Destaque foi dado ao isolado 307 que obteve o menor IVCM médio, enquanto que os isolados 318 e 646 apresentaram maior IVCM médio (p<0,05). Assim, concluiu-se que houve variabilidade entre os isolados de C. cassiicola para o IVCM em relação à temperatura, devendo ser observado a faixa ótima de crescimento para cada isolado

AR/ER Ratio Correlates with Expression of Proliferation Markers and with Distinct Subset of Breast Tumors

The co-expression of androgen (AR) and estrogen (ER) receptors, in terms of higher AR/ER ratio, has been recently associated with poor outcome in ER-positive (ER+) breast cancer (BC) patients. The aim of this study was to analyze if the biological aggressiveness, underlined in ER+ BC tumors with higher AR/ER ratio, could be due to higher expression of genes related to cell proliferation. On a cohort of 47 ER+ BC patients, the AR/ER ratio was assessed by immunohistochemistry and by mRNA analysis. The expression level of five gene proliferation markers was defined through TaqMan&reg;-qPCR assays. Results were validated using 979 BC cases obtained from gene expression public databases. ER+ BC tumors with ratios of AR/ER &ge; 2 have higher expression levels of cellular proliferation genes than tumors with ratios of AR/ER &lt; 2, in both the 47 ER+ BC patients (P &lt; 0.001) and in the validation cohort (P = 0.005). Moreover, BC cases with ratios of AR/ER &ge; 2 of the validation cohort were mainly assigned to luminal B and HER2-enriched molecular subtypes, typically characterized by higher proliferation and poorer prognosis. These data suggest that joint routine evaluation of AR and ER expression may identify a unique subset of tumors, which show higher levels of cellular proliferation and therefore a more aggressive behavior

Polyglucosan Body Structure in Lafora Disease

Abnormal carbohydrate structures known as polyglucosan bodies (PGBs) are associated with neurodegenerative disorders, glycogen storage diseases (GSDs), and aging. A hallmark of the GSD Lafora disease (LD), a fatal childhood epilepsy caused by recessive mutations in the EPM2A or EPM2B genes, are cytoplasmic PGBs known as Lafora bodies (LBs). LBs result from aberrant glycogen metabolism and drive disease progression. They are abundant in brain, muscle and heart of LD patients and Epm2a-/- and Epm2b-/- mice. LBs and PGBs are histologically reminiscent of starch, semicrystalline carbohydrates synthesized for glucose storage in plants. In this study, we define LB architecture, tissue-specific differences, and dynamics. We propose a model for how small polyglucosans aggregate to form LBs. LBs are very similar to PGBs of aging and other neurological disorders, and so these studies have direct relevance to the general understanding of PGB structure and formation

Growth of the Eucalyptus urograndis, Schizolobium parahyba var. amazonicum, Tectona grandis and Ochroma pyramidale on integration crop-livestock-forest systems in the Amazon Biome.

The objective of this study was to evaluate the growth in diameter and height eucalyptus (hybrid Eucalyptus urograndis), paricá (Schizolobium parahyba var. amazonicum), teak (Tectona grandis) and balsa tree (Ochroma pyramidale), seeking to supply information about the use of these species in crop-livestock-forest integration (ICLF) systems