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The effect of lipidation on the self-assembly of the gut-derived peptide hormone PYY3–36
Lipidation is a powerful strategy to improve the stability in vivo of peptide drugs. Attachment of a lipid chain to a hydrophilic peptide leads to amphiphilicity and the potential for surfactant-like self-assembly. Here, the self-assembly and conformation of three lipidated derivatives of the gastrointestinal peptide hormone PYY3–36 is examined using a comprehensive range of spectroscopic, scattering, and electron microscopy methods and compared to those of the parent PYY3–36 peptide. The peptides are lipidated at Ser(11), Arg(17), or Arg(23) in the peptide; the former is within the β-turn domain (based on the published solution NMR structure), and the latter two are both within the α-helical domain. We show that it is possible to access a remarkable diversity of nanostructures ranging from micelles to nanotapes and fibrillar hydrogels by control of assembly conditions (concentration, pH, and temperature). All of the lipopeptides self-assemble above a critical aggregation concentration (cac), determined through pyrene fluorescence probe measurements, and they all have predominantly α-helical secondary structure at their native pH. The pH and temperature dependence of the α-helical conformation were probed via circular dichroism spectroscopy experiments. Lipidation was found to provide enhanced stability against changes in temperature and pH. The self-assembled structures were investigated using small-angle X-ray scattering (SAXS) and cryogenic transmission electron microscopy (cryo-TEM). Distinct differences in nanostructure were observed for lipidated and unlipidated peptides, also depending on the position of lipidation. Remarkably, micelles containing lipopeptides with α-helical peptide conformation were observed. Gelation was observed at higher concentrations in certain pH intervals for the lipidated peptides, but not for unlipidated PYY3–36. Thus, lipidation, in addition to enhancing stability against pH and temperature variation, also provides a route to prepare PYY peptide hydrogels. These findings provide important insights into the control of PYY3–36 conformation and aggregation by lipidation, relevant to the development of future therapeutics based on this peptide hormone, for example, in treatments for obesity
The Effect of Lipidation on the Self-Assembly of the Gut-Derived Peptide Hormone PYY<sub>3–36</sub>
Lipidation
is a powerful strategy to improve the stability in vivo
of peptide drugs. Attachment of a lipid chain to a hydrophilic peptide
leads to amphiphilicity and the potential for surfactant-like self-assembly.
Here, the self-assembly and conformation of three lipidated derivatives
of the gastrointestinal peptide hormone PYY<sub>3–36</sub> is
examined using a comprehensive range of spectroscopic, scattering,
and electron microscopy methods and compared to those of the parent
PYY<sub>3–36</sub> peptide. The peptides are lipidated at Ser(11),
Arg(17), or Arg(23) in the peptide; the former is within the β-turn
domain (based on the published solution NMR structure), and the latter
two are both within the α-helical domain. We show that it is
possible to access a remarkable diversity of nanostructures ranging
from micelles to nanotapes and fibrillar hydrogels by control of assembly
conditions (concentration, pH, and temperature). All of the lipopeptides
self-assemble above a critical aggregation concentration (cac), determined
through pyrene fluorescence probe measurements, and they all have
predominantly α-helical secondary structure at their native
pH. The pH and temperature dependence of the α-helical conformation
were probed via circular dichroism spectroscopy experiments. Lipidation
was found to provide enhanced stability against changes in temperature
and pH. The self-assembled structures were investigated using small-angle
X-ray scattering (SAXS) and cryogenic transmission electron microscopy
(cryo-TEM). Distinct differences in nanostructure were observed for
lipidated and unlipidated peptides, also depending on the position
of lipidation. Remarkably, micelles containing lipopeptides with α-helical
peptide conformation were observed. Gelation was observed at higher
concentrations in certain pH intervals for the lipidated peptides,
but not for unlipidated PYY<sub>3–36</sub>. Thus, lipidation,
in addition to enhancing stability against pH and temperature variation,
also provides a route to prepare PYY peptide hydrogels. These findings
provide important insights into the control of PYY<sub>3–36</sub> conformation and aggregation by lipidation, relevant to the development
of future therapeutics based on this peptide hormone, for example,
in treatments for obesity
Preclinical evidence for the role of stem/stromal cells in COPD
Chronic obstructive pulmonary disease (COPD) is one of the leading causes of death worldwide and there are currently limited treatment options for these patients. The disease is characterized by a reduction in airflow due to chronic bronchitis, as well as airspace enlargement in the distal lung, resulting in a loss of surface area available for gas exchange. At end-stage disease, oxygen therapy and lung transplantation remain the only potential options. The disease is heterogeneous and both inflammatory cells as well as structural cells are thought to play a role in disease onset and progression. Pharmaceutical approaches are ineffective at reversing disease pathology and currently aim only to provide symptomatic relief. A recent area of investigation focuses on exogenous cell therapy, including stem cell administration, and its potential for directing lung regeneration. Cell therapies from a variety of sources, as well as cell-derived products such as extracellular vesicles, have recently shown efficacy in animal models of COPD, but early clinical trials have not yet shown efficacy. In this chapter, we discuss the different animal models of COPD as well as the studies which have been conducted to date with cell therapies. We conclude the chapter with a discussion regarding the limitations of current animal models and discuss potential areas for future study