Design of the experimental procedures.

<p>Design of the experimental procedures.</p

Kaplan Meier survival curves of guinea pigs after EBOV infection, according to treatment with anti-EBOV IgGs.

<p>A: Survival of guinea pigs having received the anti-EBOV IgGs (n = 10) compared to guinea pigs receiving the non-immune DKO IgGs (n = 5, *p = 0.0424, using a Log Rank test). B: Survival of guinea pigs having received one (DO) or two (D0 and D3) doses of polyclonal anti-EBOV IgGs.</p

Viral loads in serum at day 3 post-EBOV infection.

<p>A: Levels of circulating EBOV transcripts at day 3 post infection in the serum of each animal was evaluated by RT-qPCR using primers targeting EBOV polymerase gene. The horizontal bars represent the median values. The median D3 virus load was 839,333 following the injection of non-immune IgGs from a DKO pig and 259 following the injection of anti-EBOV antibodies (p = 0.055, using a Mann-Whitney test). The limit of detection of the test was of 180 relative genome copies/ml, and all values under this threshold were considered as negative data (as represented on the graph). B: Correlation between viral load on day 3 and guinea pig survival. Kendall's rank correlation coefficient showed a significant negative correlation (p = 0.0003) between EBOV viral load at day 3 and survival following infection, when considering all pooled data. Non-treated animal’s values are displayed as circles whereas treated animal’s values are displayed as squares.</p

Effect of the immunization of double KO pig with EBOV VLPs.

<p>Serum samples obtained from an immunized double KO pig before (day 0), during (day 15, 30 and 57) or after immunizations (day 83) were analyzed using ELISA for specific anti-GP antibodies. The mean OD at each time point for each dilution is represented. Anti-EBOV GP titers of the latest collected pig sera was about 1:100,000.</p