Rancang Bangun Aplikasi Perhitungan Indeks Massa Tubuh Sebagai Salah Satu Dasar Aplikasi Diet Diabetes

Untuk mencegah komplikasi yang dapat terjadi akibat diabetes mellitus, maka kadar gula dalam darah harus dijaga agar berada dalam rentang yang normal, salah satu caranya adalah dengan mengatur pola makan penderita diabetes mellitus. Aplikasi Diet Diabetes Diabetes Berdasarkan Nilai BMI (Body Mass Index) yang membutuhkan input manual selain kurang praktis, kesalahan indentifikasi dengan perkiraan juga akan mempengaruhi besar nilai perhitungan yang tidak sesuai.Untuk itu agar lebih praktis mendapatkan nilai BMI dan mengurangi kesalahan identifikasi dibuatlah aplikasi yang menggunakan sensor ultrasonik untuk menggukur tinggi badan, load cell untuk mengukur berat badan, dan mikrokontroller untuk mengolah sensor sehingga nantinya akan diketahui nilai BMI seseorang yang akan dikonversi dalam tabel jenis diet diabetes serta komposisi zat gizi yang terkandung.Pengujian akhir menunjukkan kerja yang sangat baik dengan prosentase kesalahan sensor ultrasonik 0,09% dan load cell setelah dikuatkan sebesar 0,37%. Dan program sistem jenis diet diabetes serta komposisi zat gizi yang terkandung dapat ditampilkan sesuai dengan yang diinginkan.Kata kunci – diabetes mellitus, BMI, sensor ultrasonik, loADCell

Provenance and tectonic setting of the protoliths of the Metamorphic Complexes of Sierra de San Luis

A major and trace element based characterization of the metasedimentary protoliths of three metamorphic units of Sierra de San Luis (Pringles Metamorphic Complex, San Luis Formation and Conlara Metamorphic Complex) is presented. Geochemistry indicates a dominance of shales in the protoliths of San Luis Formation, whereas greywackes and shales made up the Conlara Metamorphic complex and mainly greywackes, the Pringles Metamorphic Complex. Both major element data and trace element ratios (i.e. Th/Sc, Th/U,) indicate a source with an average upper crustal composition for the protoliths of the Pringles Metamorphic Complex, the San Luis Formation and the shales of the Conlara Metamorphic complex. A component with less evolved signature may be inferred for the metagreywackes of the Conlara metamorphic Complex. Mixed sourced detritus are indicated for the three units with clastic material resulting mainly from both andesitic and acidic/recycled detritus. The overall data consistently suggest a continental island arc and/or active margin setting as the more probable geodynamic scenario for the deposition of the sedimentary precursors of the studied units. In this context, a back-arc setting can account for the mixed nature of the inferred source areas with uplifted old basement and arc-related detritus as the end members of the mixtures. The inferred back-arc basin would have evolved through the Cambrian receiving the sediments derived from the Pampean Orogen to the east combined with probably some old crust exposures and to the west the source might have been controlled by the active continental margin. © 2003 Asociación Geológica Argentina.Fil:Cerredo, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina

Loop-Mediated Isothermal Amplification (LAMP) and SYBR Green qPCR for Fast and Reliable Detection of Geosmithia morbida (Kolařik) in Infected Walnut

Walnut species (Juglans spp.) are multipurpose trees, widely employed in plantation forestry for high-quality timber and nut production, as well as in urban greening as ornamental plants. These species are currently threatened by the thousand cankers disease (TCD) complex, an insect–fungus association which involves the ascomycete Geosmithia morbida (GM) and its vector, the bark beetle Pityophthorus juglandis. While TCD has been studied extensively where it originated in North America, little research has been carried out in Europe, where it was more recently introduced. A key step in research to cope with this new phytosanitary emergency is the development of effective molecular detection tools. In this work, we report two accurate molecular methods for the diagnosis of GM, based on LAMP (real-time and visual) and SYBR Green qPCR, which are complimentary to and integrated with similar recently developed assays. Our protocols detected GM DNA from pure mycelium and from infected woody tissue with high accuracy, sensitivity, and specificity, without cross-reactivity to a large panel of taxonomically related species. The precision and robustness of our tests guarantee high diagnostic standards and could be used to support field diagnostic end-users in TCD monitoring and surveillance campaigns

Hyperthermophilic Aquifex aeolicus initiates primer synthesis on a limited set of trinucleotides comprised of cytosines and guanines

The placement of the extreme thermophile Aquifex aeolicus in the bacterial phylogenetic tree has evoked much controversy. We investigated whether adaptations for growth at high temperatures would alter a key functional component of the replication machinery, specifically DnaG primase. Although the structure of bacterial primases is conserved, the trinucleotide initiation specificity for A. aeolicus was hypothesized to differ from other microbes as an adaptation to a geothermal milieu. To determine the full range of A. aeolicus primase activity, two oligonucleotides were designed that comprised all potential trinucleotide initiation sequences. One of the screening templates supported primer synthesis and the lengths of the resulting primers were used to predict possible initiation trinucleotides. Use of trinucleotide-specific templates demonstrated that the preferred initiation trinucleotide sequence for A. aeolicus primase was 5′-d(CCC)-3′. Two other sequences, 5′-d(GCC)-3′ and d(CGC)-3′, were also capable of supporting initiation, but to a much lesser degree. None of these trinucleotides were known to be recognition sequences used by other microbial primases. These results suggest that the initiation specificity of A. aeolicus primase may represent an adaptation to a thermophilic environment

Effect of High-Titer Convalescent Plasma on Progression to Severe Respiratory Failure or Death in Hospitalized Patients with COVID-19 Pneumonia: A Randomized Clinical Trial

Importance: Convalescent plasma (CP) has been generally unsuccessful in preventing worsening of respiratory failure or death in hospitalized patients with COVID-19 pneumonia. Objective: To evaluate the efficacy of CP plus standard therapy (ST) vs ST alone in preventing worsening respiratory failure or death in patients with COVID-19 pneumonia. Design, Setting, and Participants: This prospective, open-label, randomized clinical trial enrolled (1:1 ratio) hospitalized patients with COVID-19 pneumonia to receive CP plus ST or ST alone between July 15 and December 8, 2020, at 27 clinical sites in Italy. Hospitalized adults with COVID-19 pneumonia and a partial pressure of oxygen-to-fraction of inspired oxygen (Pao2/Fio2) ratio between 350 and 200 mm Hg were eligible. Interventions: Patients in the experimental group received intravenous high-titer CP (≥1:160, by microneutralization test) plus ST. The volume of infused CP was 200 mL given from 1 to a maximum of 3 infusions. Patients in the control group received ST, represented by remdesivir, glucocorticoids, and low-molecular weight heparin, according to the Agenzia Italiana del Farmaco recommendations. Main Outcomes and Measures: The primary outcome was a composite of worsening respiratory failure (Pao2/Fio2ratio <150 mm Hg) or death within 30 days from randomization. Results: Of the 487 randomized patients (241 to CP plus ST; 246 to ST alone), 312 (64.1%) were men; the median (IQR) age was 64 (54.0-74.0) years. The modified intention-to-treat population included 473 patients. The primary end point occurred in 59 of 231 patients (25.5%) treated with CP and ST and in 67 of 239 patients (28.0%) who received ST (odds ratio, 0.88; 95% CI, 0.59-1.33; P =.54). Adverse events occurred more frequently in the CP group (12 of 241 [5.0%]) compared with the control group (4 of 246 [1.6%]; P =.04). Conclusions and Relevance: In patients with moderate to severe COVID-19 pneumonia, high-titer anti-SARS-CoV-2 CP did not reduce the progression to severe respiratory failure or death within 30 days. Trial Registration: ClinicalTrials.gov Identifier: NCT04716556