Ecological Processes Shaping Bulk Soil and Rhizosphere Microbiome Assembly in a Long-Term Amazon Forest-to-Agriculture Conversion.

Forest-to-agriculture conversion has been identified as a major threat to soil biodiversity and soil processes resilience, although the consequences of long-term land use change to microbial community assembly and ecological processes have been often neglected. Here, we combined metagenomic approach with a large environmental dataset, to (i) identify the microbial assembly patterns and, (ii) to evaluate the ecological processes governing microbial assembly, in bulk soil and soybean rhizosphere, along a long-term forest-to-agriculture conversion chronosequence, in Eastern Amazon. We hypothesized that (i) microbial communities in bulk soil and rhizosphere have different assembly patterns and (ii) the weight of the four ecological processes governing assembly differs between bulk soil and rhizosphere and along the chronosequence in the same fraction. Community assembly in bulk soil fitted most the zero-sum multinomial (ZSM) neutral-based model, regardless of time. Low to intermediate dispersal was observed. Decreasing influence of abiotic factors was counterbalanced by increasing influence of biotic factors, as the chronosequence advanced. Undominated ecological processes of dispersal limitation and variable selection governing community assembly were observed in this soil fraction. For soybean rhizosphere, community assembly fitted most the lognormal niche-based model in all chronosequence areas. High dispersal and an increasing influence of abiotic factors coupled with a decreasing influence of biotic factors were found along the chronosequence. Thus, we found a dominant role of dispersal process governing microbial assembly with a secondary effect of homogeneous selection process, mainly driven by decreasing aluminum and increased cations saturation in soil solution, due to long-term no-till cropping. Together, our results indicate that long-term no-till lead community abundances in bulk soil to be in a transient and conditional state, while for soybean rhizosphere, community abundances reach a periodic and permanent distribution state. Dominant dispersal process in rhizosphere, coupled with homogeneous selection, brings evidences that soybean root system selects microbial taxa via trade-offs in order to keep functional resilience of soil processes

Responses of soil microbial biomass and enzyme activity to herbicides imazethapyr and flumioxazin

The use of herbicides is important for controlling weeds in crops. However, they can present impacts on soil properties, such as biological properties. In this study, we evaluated the responses of soil microbial biomass and enzymes activity to the application of the herbicides imazethapyr and flumioxazin and their mixture in an experiment under laboratory conditions, using soils with a different history of use. Soil microbial biomass C (MBC) decreased, while microbial biomass N (MBN) was not affected after the application of the herbicides as compared to the control. Soil respiration, respiratory quotient, and dehydrogenase (DHA) activity increased significantly after the application of the herbicides compared to the control. The hydrolysis of fluorescein diacetate (FDA) was not significantly different between the control and the herbicide treatments. The principal response curve showed the largest initial effects for the flumioxazin, followed by imazethapyr and their mixture. Flumioxazin had a different influence on soil respiration and respiratory quotient than imazethapyr and their mixture. Finally, the effects of herbicides on soil microbial biomass and enzymes are short-term as we observed recovery in the biological parameters over time.</p

Evaluation of the bond strength at the resin-dentin interface using self-etching adhesive systems containing different functional monomers / Avaliação da resistência de união na interface resina-dentina usando sistemas adesivos autocondicionantes contendo diferentes monômeros funcionais

Background: Self-etching adhesive systems have attracted considerable interest in dentistry, as these materials simplify the restorative protocol and allow a less sensitive adhesive technique. The objective of the present study was to analyze in vitro the performance of self-etching adhesive systems containing different functional monomers. Materials and Methods:  The adhesives evaluated were: Clearfil SE Bond (CSE) adhesive containing 10-MDP, Optibond All-in-One (OPT) containing GPDM, Palfique Bond (PB) containing 3D-SR monomer. Immediate microtensite bond strength (mTBS) and after thermocycling were realized. Interaction between the functional monomers and dentin was characterized using thin-film X-ray diffraction (TF-XRD) and scanning electron microscopy (SEM). In the experiments were used 60 molars. Thirty molars were divided into 3 experimental conditions (n=10) according to the adhesive system; the teeth were restored and sectioned on “toothpick” specimens for mTBS. Additionally, five teeth were prepared in the form of discs and the adhesives applied without light curing on the surface for XRD analysis. For morphological evaluation in SEM, 25 teeth were treated with the respective adhesives. The mTBS data were submitted to ANOVA two factors and Tukey's test (5%).  Results: The adhesive vs. time interaction was statistically significant (p&lt;0.001), with the CSE adhesive obtaining the highest means and being the worst performance of BP. The analysis of XRD found that the monomers interact chemically with hydroxyapatite, but with different intensities. SEM images indicate that the applied adhesives infiltrate the dentin forming resin tags in greater or lesser amounts. Conclusions: The different functional monomers have a direct impact on the effectiveness of the adhesive bond, with the 10-MDP monomer, the CSE adhesive, highlighted by the higher TBS values. The self-etching adhesive systems allow a simplification in the restorative protocol, besides a less sensitive adhesive technique and less clinical time for the procedure. Therefore, knowing its mechanical and chemical properties is important to ensure longer-lasting restorations. 

Changes in the bacterial rare biosphere after permanent application of composted tannery sludge in a tropical soil

Composted tannery sludge (CTS) promotes shifts in soil chemical properties, affecting microbial communities. Although the effect of CTS application on the bacterial community has been studied, it is unclear whether this impact discriminates between the dominant and rare species. This present study investigated how the dominant and rare bacterial communities respond over time to different concentrations of CTS application (0, 2.5, 5, 10, and 20 tons/ha) for 180 days. The richness of operational taxonomic units (OTU) was 30-fold higher in the rare than in the dominant biosphere. While some phyla shifted their relative abundance differently in the dominant and rare biosphere, some genera increased their relative abundance under higher CTS concentrations, such as Nocardioides (∼100%), Rubrobacter (∼300%), and Nordella (∼400%). Undominated processes largely governed the dominant biosphere (76.97%), followed by homogeneous (12.51%) and variable (8.03%) selection, and to a lesser extent, the dispersal limitation (2.48%). The rare biosphere was driven by the CTS application as evidenced by the exclusively homogeneous selection (100%). This study showed that the rare biosphere was more sensitive to changes in soil chemical parameters due to CTS application, which evidences the importance explore this portion of the bacterial community for its biotechnological use in contaminated soils.</p

Isolation and enzyme bioprospection of bacteria associated to Bruguiera cylindrica, a mangrove plant of North Sumatra, Indonesia

Publisher Copyright: © 2021 The AuthorsMangrove-associated bacteria are of industrial interest due to their diverse and versatile enzyme properties. This study investigates the culturable bacteria from a wide range of habitat in a Bruguiera cylindrica mangrove ecosystem in North Sumatra. Screening of extracellular hydrolytic enzymes showed multiple potential traits in amylase, cellulase, chitinase, phosphatase, protease, and urease production by bacterial isolates. Molecular identification based on 16S rDNA region of a potential strain, Vibrio alginolyticus Jme3−20 is then reported as a newly proteolytic agent. The strain also showed a stable growth under salinity (NaCl) stress with considerable phosphate solubilization activities. Protease activity was enhanced by optimizing the 0.5 % (w/v) sucrose and soy peptone in the fermentation medium. SDS-PAGE and zymogram analysis showed the presence of a 35-kDa MW protease. Hence, our study revealed important insights into the bacterial diversity and activity in mangrove ecosystems, evidencing the importance of microbial exploration in this ecosystem.Peer reviewe

Impacto da pandemia de COVID-19 nas doenças respiratórias: Um estudo sobre bronquite e bronquiolite aguda em crianças na região norte do Brasil

Respiratory diseases constitute a diverse set of conditions that affect the respiratory system, playing a significant role in healthcare systems. Bronchitis and acute bronchiolitis are two respiratory conditions that significantly impact lung health, especially in young children and some vulnerable groups. The association between bronchitis, acute bronchiolitis and COVID-19 is significant due to the similarities in the respiratory symptoms of these conditions. Both involve inflammation of the airways, resulting in coughing and difficulty breathing. Therefore, it is important to analyse epidemiological profiles before and after the pandemic and understand the impact of the pandemic on hospitalization and deaths associated with bronchitis and bronchiolitis. The main objective of this article was to analyse the prevalence and epidemiological profile of hospitalizations and deaths caused by bronchitis and acute bronchiolitis in children living in the Northern region of Brazil. This is a quantitative, retrospective study that analysed the number of hospitalizations and deaths due to bronchitis and acute bronchiolitis in the North region based on public data from the Hospital Information System (SIH) of the Unified Health System (SUS). During the analysis period, the number of hospitalizations decreased by 6%, but the number of deaths increased significantly. The state of Pará has the highest number of hospitalizations, and the state of Amazonas has the highest mortality rate. Both predominate in children under 1 year of age. Additionally, hospital spending increased by 32% during the COVID-19 pandemic. The significant incidence of bronchitis and acute bronchiolitis in children under one year of age stands out as a primary concern for child health. The inherent vulnerability of this age group, combined with frequent exposure to infectious agents, highlights the need for more effective preventive approaches and management strategies.As doenças respiratórias constituem um conjunto diversificado de condições que afetam o sistema respiratório, desempenhando um papel significativo nos sistemas de saúde. A bronquite e bronquiolite aguda são duas condições respiratórias que impactam significativamente a saúde pulmonar, especialmente em crianças pequenas e em alguns grupos vulneráveis. A associação entre bronquite, bronquiolite aguda e COVID-19 é significativa devido às semelhanças nos sintomas respiratórios dessas condições. Ambos envolvem inflamação das vias respiratórias, resultando em tosse e dificuldade respiratória. Dessa forma, é importante analisar perfis epidemiológicos antes e depois da pandemia e compreender o impacto da pandemia na hospitalização e óbitos associados à bronquite e bronquiolite. O objetivo principal deste artigo foi analisar a prevalência e o perfil epidemiológico das internações e óbitos causados por bronquite e bronquiolite aguda em crianças residentes na região Norte do Brasil. Trata-se de um estudo quantitativo, retrospectivo, que analisou o número de internações e óbitos por bronquites e bronquiolite aguda na região Norte com base em dados públicos do Sistema de Informações Hospitalares (SIH) do Sistema Único de Saúde (SUS). Durante o período de análise, o número de hospitalizações diminuiu 6%, mas o número de mortes aumentou significativamente. O estado do Pará tem o maior número de internações, e o estado do Amazonas tem a maior taxa de mortalidade. Ambos predominam em crianças menores de 1 ano. Além disso, os gastos hospitalares aumentaram 32% durante a pandemia de COVID-19. A significativa incidência de bronquites e bronquiolite aguda em crianças com menos de um ano destaca-se como uma preocupação primordial para a saúde infantil. A vulnerabilidade inerente a esse grupo etário, combinada com a exposição frequente a agentes infecciosos, evidencia a necessidade de abordagens preventivas e estratégias de manejo mais eficazes

Represas de abastecimento público de Juiz de Fora: mananciais da vida

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ATENÇÃO FARMACÊUTICA NO CONTEXTO DO TRATAMENTO DE TABAGISTAS:: ESTUDO DE CASO

Medicamentos são substâncias químicas que, quando em doses terapêuticas, atuam no organismo contribuindo para homeostase. Em contrapartida, sabe-se que a associação de dois ou mais fármacos pode acarretar alterações em seu mecanismo de atuação, conduzindo a riscos de toxicidade acompanhados de prejuízos à saúde por meio das interações medicamentosas. Objetiva-se estudar a atenção farmacêutica no contexto do tratamento de tabagistas. Na presente pesquisa foi realizado seguimento farmacoterapêutico de um paciente atendido em grupo de tratamento de tabagistas do município de Campina Grande, Paraíba. O tabagista em tratamento voluntário, sexo masculino, 52 anos, infartado, usuário dos medicamentos trimetazidina, bissulfato de clopidogrel, ácido acetilsalicílico, atorvastatina cálcica, oxalato de escitalopram, ramipril e succinato de metoprolol. Foi prescrito Bupropiona para tratamento do tabagismo. No acompanhamento da evolução foi utilizado o método Dáder, participação em grupos de apoio para resolução dos Problemas Relacionados a Medicamentos (PRMs) e aprimoramento da prática de atenção farmacêutica. Observaram-se riscos à saúde decorrentes da interação entre fármacos, fazendo-se necessárias estratégias que proporcionem segurança à utilização dos mesmos. Foi suspensa bupropiona e realizada a abordagem cognitiva comportamental. No relato, o tabagista em tratamento recebeu orientações por meio da atenção farmacêutica fortalecendo e promovendo adesão ao tratamento sem utilização da medicação prescrita para tratamento de tabagistas. Conclui-se que a atenção farmacêutica tem potencial para se tornar indispensável no âmbito da saúde em virtude de promover saúde por realizar observação qualificada da atuação do