Development of welding techniques and filler metals for high strength aluminum alloys second quarterly report, 1 oct. - 31 dec. 1964

Welding techniques and filler metals for high strength aluminum alloys evaluated by bulge test progra

Osteoclastic Resorption of Calcium Phosphate Ceramic Thin Films

Sub-micron calcium phosphate ceramic thin films were formed by vertically dipping transparent quartz plates in a particulate sol-gel suspension. Primary adult rat bone marrow cell populations were cultured on the ceramic thin films in conditions known to allow the differentiation of cells of the osteoclast lineage. Monitoring the cultures for periods of 11 to 28 days revealed the creation of resorption lacunae in the thin films by multinucleate cells. Some cultures were heated at 42 °C overnight to remove adherent cells; using bright field light microscopy (LM), after staining with silver nitrate, the degree of resorption could be easily assessed. Other cultures were fixed and stained for tartrate-resistant acid phosphatase (TRAP) activity and prepared for LM and/ or scanning electron microscopy (SEM). Examination of the cultures, following fixation, showed the multinucleate cells associated with resorption lacunae to be TRAP positive. The nuclearity of the cells varied considerably. SEM showed that the cells had resorbed the thin films to produce discrete resorption lacunae similar to those found in normal bone tissue. From their morphology, TRAP positive staining, and resorptive activity, the cells were considered to be osteoclasts. The size of individual or combined lacunae varied from \u3c 10 μm to - 1 mm. These thin film culture substrata may be employed to investigate the function of individual resorbing cells or, especially after removal of the adherent cell layer, easily quantify resorption which is the major functional activity of osteoclasts. We conclude that these thin film ceramic culture substrata can be used as alternatives to bone slices in osteoclast resorption assays and thus could be employed to investigate both the functional and metabolic activities of osteoclasts

Development of welding techniques and filler metals for high strength aluminum alloys Annual summary report, 27 Jun. 1964 - 27 Jun. 1965

Welding techniques and filler metals for high strength aluminum alloy

A cloned linguistic decision tree controller for real-time path planning in hostile environments

AbstractThe idea of a Cloned Controller to approximate optimised control algorithms in a real-time environment is introduced. A Cloned Controller is demonstrated using Linguistic Decision Trees (LDTs) to clone a Model Predictive Controller (MPC) based on Mixed Integer Linear Programming (MILP) for Unmanned Aerial Vehicle (UAV) path planning through a hostile environment. Modifications to the LDT algorithm are proposed to account for attributes with circular domains, such as bearings, and discontinuous output functions. The cloned controller is shown to produce near optimal paths whilst significantly reducing the decision period. Further investigation shows that the cloned controller generalises to the multi-obstacle case although this can lead to situations far outside of the training dataset and consequently result in decisions with a high level of uncertainty. A modification to the algorithm to improve the performance in regions of high uncertainty is proposed and shown to further enhance generalisation. The resulting controller combines the high performance of MPC–MILP with the rapid response of an LDT while providing a degree of transparency/interpretability of the decision making

Bifurcation Analysis Using Rigorous Branch and Bound Methods

For the study of nonlinear dynamic systems, it is important to locate the equilibria and bifurcations occurring within a specified computational domain. This paper proposes a new approach for solving these problems and compares it to the numerical continuation method. The new approach is based upon branch and bound and utilizes rigorous enclosure techniques to yield outer bounding sets of both the equilibrium and local bifurcation manifolds. These sets, which comprise the union of hyper-rectangles, can be made to be as tight as desired. Sufficient conditions for the existence of equilibrium and bifurcation points taking the form of algebraic inequality constraints in the state-parameter space are used to calculate their enclosures directly. The enclosures for the bifurcation sets can be computed independently of the equilibrium manifold, and are guaranteed to contain all solutions within the computational domain. A further advantage of this method is the ability to compute a near-maximally sized hyper-rectangle of high dimension centered at a fixed parameter-state point whose elements are guaranteed to exclude all bifurcation points. This hyper-rectangle, which requires a global description of the bifurcation manifold within the computational domain, cannot be obtained otherwise. A test case, based on the dynamics of a UAV subject to uncertain center of gravity location, is used to illustrate the efficacy of the method by comparing it with numerical continuation and to evaluate its computational complexity

Wind tunnel testing of a high aspect ratio wing model

There is much current interest in the development of High Aspect Ratio Wing (HARW) designs for improved aircraft performance. However, there are a lack of relevant data sets available to validate aeroelastic modelling approaches for highly flexible wings. The design and manufacture of a highly flexible 2.4m semi-span wing is described. A series of low speed wind tunnel tests were performed to generate displacement, acceleration, strain gauge, aerodynamic pressure and six component balance measurements for a range of airspeeds and wing root angles of attack. Numerous static and dynamic measurements were made. Preliminary results are shown for the static and dynamic, structural and aerodynamic behaviour over a range of different airspeeds and wing root angles of attack.Peer ReviewedPostprint (published version

Regulation of haemopoietic stem‐cell proliferation in mice carrying the Slj allele

We investigated a haemopoietic stromal defect, in mice heterozygous for the Slj allele, during haemopoietic stress induced by treatment with bacterial lipopolysaccharides (LPS) or lethal total body irradiation (TBI) and bone‐marrow cell (BMC) reconstitution. Both treatments resulted in a comparable haemopoietic stem cell (CFU‐s) proliferation in Slj/+ and +/+ haemopoietic organs. There was no difference in committed haemopoietic progenitor cell (BFU‐e and CFU‐G/M) kinetics after TBI and +/+ bone‐marrow transplantation in Slj/+ and +/+ mice. the Slj/+ mice were deficient in their ability to support macroscopic spleen colony formation (65% of +/+ controls) as measured at 7 and 10 days after BMC transplantation. However, the Slj/+ spleen colonies contained the same number of BFU‐E and CFU‐G/M as colonies from +/+ spleens, while their CFU‐s content was increased. On day 10 post‐transplantation, the macroscopic ‘missing’ colonies could be detected at the microscopic level. These small colonies contained far fewer CFU‐s than the macroscopic detectable colonies. Analysis of CFU‐s proliferation‐inducing activities in control and post‐LPS sera revealed that Slj/+ mice are normal in their ability to produce and to respond to humoral stem‐cell regulators. We postulate that Slj/+ mice have a normal number of splenic stromal ‘niches’ for colony formation. However, 35% of these niches is defective in its proliferative support. Copyrigh

Autonomous growth potential of leukemia blast cells is associated with poor prognosis in human acute leukemias

We have described a severe combined immunodeficiency (SCID) mouse model that permits the subcutaneous growth of primary human acute leukemia blast cells into a measurable subcutaneous nodule which may be followed by the development of disseminated disease. Utilizing the SCID mouse model, we examined the growth potential of leukemic blasts from 133 patients with acute leukemia, (67 acute lymphoblastic leukemia (ALL) and 66 acute myeloid leukemia (AML)) in the animals after subcutaneous inoculation without conditioning treatment. The blasts displayed three distinct growth patterns: "aggressive", "indolent", or "no tumor growth". Out of 133 leukemias, 45 (33.8%) displayed an aggressive growth pattern, 14 (10.5%) displayed an indolent growth pattern and 74 (55.6%) did not grow in SCID mice. The growth probability of leukemias from relapsed and/or refractory disease was nearly 3 fold higher than that from patients with newly diagnosed disease. Serial observations found that leukemic blasts from the same individual, which did not initiate tumor growth at initial presentation and/or at early relapse, may engraft and grow in the later stages of disease, suggesting that the ability of leukemia cells for engraftment and proliferation was gradually acquired following the process of leukemia progression. Nine autonomous growing leukemia cell lines were established in vitro. These displayed an aggressive proliferation pattern, suggesting a possible correlation between the capacity of human leukemia cells for autonomous proliferation in vitro and an aggressive growth potential in SCID mice. In addition, we demonstrated that patients whose leukemic blasts displayed an aggressive growth and dissemination pattern in SClD mice had a poor clinical outcome in patients with ALL as well as AML. Patients whose leukemic blasts grew indolently or whose leukemia cells failed to induce growth had a significantly longer DFS and more favorable clinical course