Measurement of grapevine canopy leaf area by using an ultrasonic-based method.

Aim: Measurement of leaf area in grapevine has always been a critical point in researches focused on irrigation management, training systems, source-sink interrelationships and efficiency of spray application to canopies. In this work, we propose the use of ultrasonic sensors as a fast and accurate tool for the estimation of large portions of leaf canopy area. Methods and results: Through outputs of ultrasonic sensors installed on a tractor moving along vineyard rows, we calculated an ultrasonic-based leaf density index that we correlated with three measurements or estimates of canopy area: I) direct measurement of the area of a canopy portion (LAØ), assessed by summing the areas of all the leaves, where each single-leaf area was assessed by regressing the leaf diameter (the maximum width perpendicular to the main rip) against the related leaf area calculated on the basis of a relation between the leaf diameter and the leaf area, previously assessed through an area meter on a 20-leaf sample; II) the point quadrat output (LApq); and III) the canopy leaf area index (LAI) obtained through LAI-2000 (Li-Cor) technology. The measurements were assessed on six cultivars in three replicate rows (8-12 plants per cultivar per row) in a vineyard trained to a vertical trellis system. Conclusion: When we correlated the three independent control parameters with each other, we obtained highly significant correlations between LApq and LAØ, but less significant correlations between these two and LAI-2000 outputs. Also, the correlations between ultrasonic outputsoutputs and LAØ and LApq were significant, with R2 ranging between 0.84 and 0.85. On the contrary, no significant correlation was found between ultrasonic outputs and LAI-2000 outputs. These results were obtained by averaging all the values belonging to each replicated cultivar (10.5 m along the row, i.e., twelve contiguous vines); on the contrary, when the analysis was done over a shorter distance (3.5 m, i.e., four contiguous vines), the reliability of the ultrasonic-based method decreased. Significance and impact of the study: These results point to the ultrasonic technology as a powerful tool to estimate large-scale leaf canopy area, with potential applications in precision farming. At the moment, however, the limitation of this approach is the requirement of reference values for leaf area (e.g., assessed by point quadrat) to obtain absolute and not only relative outputs. With this application we can quantify, in a few hours, the canopy of a whole vineyard, in order to analyze different vegetation zones or to follow canopy development

The Oral Microbiome for Geographic Origin: An Italian Study

The human oral microbiome has primarily been studied in clinical settings and for medical purposes. More recently, oral microbial research has been incorporated into other areas of study. In forensics, research has aimed to exploit the variation in composition of the oral microbiome to answer forensic relevant topics, such as human identification and geographical provenience. Several studies have focused on the use of microbiome for continental, national, or ethnic origin evaluations. However, it is not clear how the microbiome varies between similar ethnic populations across different regions in a country. We report here a comparison of the oral microbiomes of individuals living in two regions of Italy – Lombardy and Piedmont. Oral samples were obtained by swabbing the donors’ oral mucosa, and the V4 region of the 16S rRNA gene was sequenced from the extracted microbial DNA. Additionally, we compared the oral and the skin microbiome from a subset of these individuals, to provide an understanding of which anatomical region may provide more robust results that can be useful for forensic human identification. Initial analysis of the oral microbiota revealed the presence of a core oral microbiome, consisting of nine taxa shared across all oral samples, as well as unique donor characterising taxa in 31 out of 50 samples. We also identified a trend between the abundance of Proteobacteria and Bacteroidota and the smoking habits, and of Spirochaetota and Synergistota and the age of the enrolled participants. Whilst no significant differences were observed in the oral microbial diversity of individuals from Lombardy or Piedmont, we identified two bacterial families – Corynebacteriaceae and Actinomycetaceae – that showed abundance trends between the two regions. Comparative analysis of the skin and oral microbiota showed significant differences in the alpha (p = 0.0011) and beta (Pr(>F) = 9.999e-05) diversities. Analysis of skin and oral samples from the same donor further revealed that the skin microbiome contained more unique donor characterising taxa than the oral one. Overall, this study demonstrates that whilst the oral microbiome of individuals from the same country and of similar ethnicity are largely similar, there may be donor characterising taxa that might be useful for identification purposes. Furthermore, the bacterial signatures associated with certain lifestyles could provide useful information for investigative purposes. Finally, additional studies are required, the skin microbiome may be a better discriminant for human identification than the oral one

“Touch microbiome” as a potential tool for forensic investigation: A pilot study

Human skin hosts a variety of microbes that can be transferred to surfaces (“touch microbiome”). These microorganisms can be considered as forensic markers similarly to “touch DNA”. With this pilot study, we wanted to evaluate the transferability and persistence of the “touch microbiome” on a surface after the deposition of a fingerprint and its exposure for 30 days at room temperature. Eleven volunteers were enrolled in the study. Skin microbiome samples were collected by swabbing the palm of their hands; additionally, donors were asked to touch a glass microscope slide to deposit their fingerprints, that were then swabbed. Both human and microbial DNA was isolated and quantified. Amelogenin locus and 16 human STRs were amplified, whereas the V4 region of 16 S rRNA gene was sequenced using Illumina MiSeq platform. STR profiles were successfully typed for 5 out of 22 “touch DNA” samples, while a microbiome profile was obtained for 20 out of 22 “touch microbiome” samples. Six skin core microbiome taxa were identified, as well as unique donor characterizing taxa. These unique taxa may have relevance for personal identification studies and may be useful to provide forensic intelligence information also when “touch DNA” fails. Additional future studies including greater datasets, additional time points and a greater number of surfaces may clarify the applicability of “touch microbiome” studies to real forensic contexts

Disease-specific and general health-related quality of life in newly diagnosed prostate cancer patients: The Pros-IT CNR study

Background: The National Research Council (CNR) prostate cancer monitoring project in Italy (Pros-IT CNR) is an observational, prospective, ongoing, multicentre study aiming to monitor a sample of Italian males diagnosed as new cases of prostate cancer. The present study aims to present data on the quality of life at time prostate cancer is diagnosed. Methods: One thousand seven hundred five patients were enrolled. Quality of life is evaluated at the time cancer was diagnosed and at subsequent assessments via the Italian version of the University of California Los Angeles-Prostate Cancer Index (UCLA-PCI) and the Short Form Health Survey (SF-12). Results: At diagnosis, lower scores on the physical component of the SF-12 were associated to older ages, obesity and the presence of 3+ moderate/severe comorbidities. Lower scores on the mental component were associated to younger ages, the presence of 3+ moderate/severe comorbidities and a T-score higher than one. Urinary and bowel functions according to UCLA-PCI were generally good. Almost 5% of the sample reported using at least one safety pad daily to control urinary loss; less than 3% reported moderate/severe problems attributable to bowel functions, and sexual function was a moderate/severe problem for 26.7%. Diabetes, 3+ moderate/severe comorbidities, T2 or T3-T4 categories and a Gleason score of eight or more were significantly associated with lower sexual function scores at diagnosis. Conclusions: Data collected by the Pros-IT CNR study have clarified the baseline status of newly diagnosed prostate cancer patients. A comprehensive assessment of quality of life will allow to objectively evaluate outcomes of different profile of care