3 research outputs found
Caracterización morfológica y molecular de árboles de Umbu-cajá en la región nordeste del Brasil
The umbu-cajá (Spondias sp.) is a fruit specie native of the brazilian semiarid region with high potential for cultivation. The juicy pulp is used in the production of juices and nectars. The aim of this study was to characterize 18 individuals of umbu-cajá tree at three sites located in the Brazilian northeast municipalities of Mossoró/RN and Apodi/RN to identify individuals with superior attributes for fruit processing and/or in natura consumption The morphological, physical and physicochemical characterization of 20 fruits of each individuals were performed. Leaves of trees were used for molecular analyses with Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) markers. It was possible to verify that there are phenotypic variability among the fruits of umbu-cajá trees. The individuals of the same region were grouped in the same group. JZ5, JZ6 and MA6 individuals can be used for pulp processing, while JZ1, JZ2, JZ3 and JZ6 are adequate for in natura consumption. The molecular analyses showed polymorphism for the two molecular markers used. The ISSR marker generated the greatest polymorphism among the individuals. However, the RAPD marker made it possible to achieve a more consistent cluster, which is in agreement with the geographical distribution of these individuals. The use of morphological markers together with molecular markers constitutes an important tool for characterizing umbu-cajá individuals. Keywords: genetic resources, ISSR, RAPD, Spondias sp.Umbu-cajá (Spondias sp.) es una especie de fruto nativa de la región semiárida brasileña con alto potencial de cultivo. La pulpa jugosa se utiliza en la producción de jugos y néctares. El objetivo de este estudio fue caracterizar a 18 individuos de umbu-cajá en tres sitios ubicados en los municipios noreste de Mossoró / RN y Apodi / RN para identificar individuos con atributos superiores para procesamiento de frutas y / o consumo in natura. Se realizó la caracterización morfológica, física y fisicoquímica de 20 frutos de cada individuo. Las hojas de los árboles se utilizaron para los análisis moleculares mediante Secuencias Intergénicas Repetidas Simples (ISSR) y la Amplificación aleatoria de ADN polimórfico (RAPD). Se pudo verificar la existencia de variabilidad fenotípica entre los frutos de los árboles umbu-cajá. Los individuos de la misma región se agruparon en el mismo grupo. Los individuos JZ5, JZ6 y MA6 pueden usarse para el procesamiento de pulpa, mientras que JZ1, JZ2, JZ3 y JZ6 son adecuados para el consumo in natura. Los análisis moleculares mostraron polimorfismo para los dos marcadores moleculares utilizados. El ISSR generó el mayor polimorfismo entre los individuos. Sin embargo, el marcador RAPD permitió lograr un grupo más consistente, lo que está de acuerdo con la distribución geográfica de estos individuos. El uso de marcadores morfológicos junto con marcadores moleculares constituye una herramienta importante para caracterizar individuos umbu-cajá. Palabras clave: ISSR, RAPD, recursos genéticos, Spondias sp
Função do cálcio na degradação da parede celular vegetal de frutos
Calcium is one of the most important intracellular system of plants, it remains in ionic form while inside the
cell, acting on the secondary metabolism of all cells. It is an element that plays different roles in plant tissues, especially in
protecting the cell walls and membranes and signaling in response to changing biotic and abiotic stress. The softening of the
fruit during its ripening involves changes in cell wall polysaccharides. Calcium bridges between pectic acids, or between
these and other polysaccharides hinder access and the action of pectinolytic enzymes produced by the fruit and cause
softening, and those produced by fungi and bacteria that cause decay. Calcium in plant cell wall, to covalently bind to
pectins, gives rise to calcium pectate, restricting the action of polygalacturonase and pectin, and thus slowing the softening
of fruits. The application of calcium both in spray and in the fruit results in grave: the preservation of fruit firmness,
reduction of respiration rate of respiration, reduced ethylene production, the lower activity of β-galactosidase, an increase of
hemicellulose, pectins and calcium in the pulp . In fruits, the effect of calcium has received much attention, since the
applications of this cation promotes the retardation of ripening and senescence, prolonging the life and shelf life.O cálcio é um dos elementos mais importantes no sistema intracelular de plantas, pois permanece na forma
iônica quando dentro da célula, atuando no metabolismo secundário de todas as células. É um elemento que desempenha
diferentes funções nos tecidos vegetais, notadamente na proteção das membranas e paredes celulares e na sinalização de
resposta às condições de estresse biótico ou abióticos. O amaciamento de frutos durante o seu amadurecimento implica em
modificações de polissacarídeos da parede celular. As pontes de cálcio entre os ácidos pécticos ou entre esses e outros
polissacarídeos dificultam o acesso e a ação de enzimas pectolíticas produzidas pelo fruto e que causam amaciamento, e
daquelas produzidas pelos fungos e bactérias que causam deterioração. O cálcio na parede celular vegetal, ao ligar-se
covalentemente às pectinas, dá origem ao pectato de cálcio, restringindo a ação da pectinametilesterase e poligalacturonase,
e, consequentemente, retardando o amaciamento de frutos. A aplicação de cálcio tanto em pulverização nos frutos quanto
em cova resulta em: preservação da firmeza do fruto, redução da respiração da taxa respiratória, redução da produção de
etileno, menor atividade da β-galactosidase, aumento de hemicelulose, pectinas e cálcio na polpa. Em frutos, o efeito do
cálcio tem recebido muita atenção, visto que as aplicações deste cátion promovem o retardamento da maturação e da
senescência, prolongando o período de vida útil e o tempo de prateleira
Morphological and molecular characterization of Umbu-Cajá tree in the northeast region of Brazil
The umbu-cajá (Spondias sp.) is a fruit specie native of the brazilian semiarid region with high potential for cultivation. The juicy pulp is used in the production of juices and nectars. The aim of this study was to characterize 18 individuals of umbu-cajá tree at three sites located in the Brazilian northeast municipalities of Mossoró/RN and Apodi/RN to identify individuals with superior attributes for fruit processing and/or in natura consumption The morphological, physical and physicochemical characterization of 20 fruits of each individuals were performed. Leaves of trees were used for molecular analyses with Inter Simple Sequence Repeats (ISSR) and Random Amplified Polymorphic DNA (RAPD) markers. It was possible to verify that there are phenotypic variability among the fruits of umbu-cajá trees. The individuals of the same region were grouped in the same group. JZ5, JZ6 and MA6 individuals can be used for pulp processing, while JZ1, JZ2, JZ3 and JZ6 are adequate for in natura consumption. The molecular analyses showed polymorphism for the two molecular markers used. The ISSR marker generated the greatest polymorphism among the individuals. However, the RAPD marker made it possible to achieve a more consistent cluster, which is in agreement with the geographical distribution of these individuals. The use of morphological markers together with molecular markers constitutes an important tool for characterizing umbu-cajá individuals.
Keywords: genetic resources, ISSR, RAPD, Spondias sp