5 research outputs found
Both IFN-γ and IL-4 Induce MHC Class II Expression at the Surface of Mouse Pro-B Cells
Pro-B cells are early B-cell progenitors that retain macrophage potential. We have studied
MHC class II molecules and invariant chain inducibility on four class II negative mouse pro-
B-cell clones. We analyzed the effects of IL-4 and IFN-γ, which represent the major inducers
of class II in the B-lymphoid and monocytic/macrophage lineages, respectively. After 48 h of
treatment with either cytokine, three pro-B-cell clones (C2.13, A1.5, and F2.2) expressed intracellular
invariant chain and cell-surface class II molecules. One clone (D2.1) remained negative.
As already reported, more differentiated 70Z/3 pre-B cells were inducible by IL-4 only.
These data suggest that the induction of class II and invariant-chain genes are subject to regulation
throughout B-cell differentiation
Expression of Functional MHC Class II Molecules By a Mouse Pro-B Cell Clone
We describe here the G12 pro-B cell clone that has been isolated from an IL-7 transgenic
mouse. This clone has the phenotype B220+, BP-1+ , HSA +, CD43+ λ5+ , and CD25-, and
has its Ig locus in a germline configuration. G12 cells spontaneously express cell-surface
MHC class II molecules, although to a much lesser extent than the mature M12.4.1 B-cell
lymphoma. G12 cells can process and present the native Hen Egg Lysozyme (HEL) to an
MHC class II-restricted T-cell hybridoma. The efficiency of presentation is inferior to that
obtained with M12.4.1 cells. This is the first report where a pro-B cell can serve as APC in
an MHC class II-restricted presentation
Critical residue combinations dictate peptide presentation by MHC class II molecules
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