The Role of Finite Element Analysis in Studying Potential Failure of Mandibular Reconstruction Methods

Defects of the mandible occur after trauma or resection after infection or tumours. There have been many methods espoused, but many methods can fail especially if the biomechanics of the mandible is not considered fully. As the only moveable, load-bearing bone of the skull, the mandible is subject to loads and stresses unique to it due to its shape, location and function. This chapter reviews the basic knowledge of the mandible necessary to perform finite element analysis, the challenges and then reviews several studies that have been done. The authors’ personal research is detailed to illustrate how finite element analysis can be used to look at potential failure of a new method for mandibular reconstruction and implant evaluation

Precision Spectroscopy of Polarized Molecules in an Ion Trap

Polar molecules are desirable systems for quantum simulations and cold chemistry. Molecular ions are easily trapped, but a bias electric field applied to polarize them tends to accelerate them out of the trap. We present a general solution to this issue by rotating the bias field slowly enough for the molecular polarization axis to follow but rapidly enough for the ions to stay trapped. We demonstrate Ramsey spectroscopy between Stark-Zeeman sublevels in 180Hf19F+ with a coherence time of 100 ms. Frequency shifts arising from well-controlled topological (Berry) phases are used to determine magnetic g-factors. The rotating-bias-field technique may enable using trapped polar molecules for precision measurement and quantum information science, including the search for an electron electric dipole moment.Comment: Accepted to Scienc

Sequence Requirements for the Nuclear Localization of the Murine Cytomegalovirus M44 Gene Product pp50

AbstractThe murine cytomegalovirus (MCMV) M44 gene product pp50 is normally present in the nuclei of virus-infected cells. During transient expression of pp50 in COS-1 cells, the phosphoprotein was readily detectable in the nuclei, indicating that it possesses a nuclear localization signal (NLS). Studies on the subcellular locations of N- and C-terminal deletion mutants of pp50 suggested that alterations in both the C terminus and the highly conserved N-terminal domains of pp50 affect nuclear localization. In particular, the C-terminal 11 amino acids of pp50, which includes a “KKQK” motif, were able to mediate the import of a β-galactosidase fusion protein into the nucleus. The pair of lysine residues in this motif constitutes an essential element of the C-terminal NLS as mutation of this motif to AAQK directly affected the nuclear localization of either pp50 or β-galactosidase fusion proteins containing the C-terminal portion of pp50. Furthermore our results indicated that the functionality of the C-terminal NLS is dependent on the structural integrity of the highly conserved N-terminal portion of the molecule, as deletion of amino acids 157–201 alone adversely affected nuclear localization. In the absence of a functional C-terminal NLS, the subcellular localization of pp50 is sensitive to potential conformational changes induced by mutations within the N-terminal half of the molecule. Under those circumstances, mutation of the YK residues at position 22–23 or deletion of amino acids 267–283 was sufficient to produce a protein that was impaired in nuclear import or retention

Reduction of a Vehicle Multibody Dynamic Model Using Homotopy Optimization

The original publication is available at: Hall, A., Uchida, T., Loh, F., Schmitke, C., & Mcphee, J. (2013). Reduction of a Vehicle Multibody Dynamic Model Using Homotopy Optimization. Archive of Mechanical Engineering, LX(1). https://doi.org/10.2478/meceng-2013-0002Despite the ever-increasing computational power of modern processors, the reduction of complex multibody dynamic models remains an important topic of investigation, particularly for design optimization, sensitivity analysis, parameter identification, and controller tuning tasks, which can require hundreds or thousands of simulations. In this work, we first develop a high-fidelity model of a production sports utility vehicle in Adams/Car. Single-link equivalent kinematic quarter-car (SLEKQ, pronounced “sleek”) models for the front and rear suspensions are then developed in MapleSim. To avoid the computational complexity associated with introducing bushings or kinematic loops, all suspension linkages are lumped into a single unsprung mass at each corner of the vehicle. The SLEKQ models are designed to replicate the kinematic behaviour of a full suspension model using lookup tables or polynomial functions, which are obtained from the high-fidelity Adams model in this work. The predictive capability of each SLEKQ model relies on the use of appropriate parameters for the nonlinear spring and damper, which include the stiffness and damping contributions of the bushings, and the unsprung mass. Homotopy optimization is used to identify the parameters that minimize the difference between the responses of the Adams and MapleSim models. Finally, the SLEKQ models are assembled to construct a reduced 10-degree-of-freedom model of the full vehicle, the dynamic performance of which is validated against that of the high-fidelity Adams model using four-post heave and pitch tests.The authors gratefully acknowledge the financial support provided by the Natural Sciences and Engineering Research Council of Canada (NSERC) and the NSERC/Toyota/Maplesoft Industrial Research Chair program

Peroxisome proliferator-activated receptor (PPAR)alpha expression in T cells mediates gender differences in development of T cell-mediated autoimmunity.

Peroxisome proliferator-activated receptor (PPAR)alpha is a nuclear receptor that mediates gender differences in lipid metabolism. PPARalpha also functions to control inflammatory responses by repressing the activity of nuclear factor kappaB (NF-kappaB) and c-jun in immune cells. Because PPARalpha is situated at the crossroads of gender and immune regulation, we hypothesized that this gene may mediate sex differences in the development of T cell-mediated autoimmune disease. We show that PPARalpha is more abundant in male as compared with female CD4(+) cells and that its expression is sensitive to androgen levels. Genetic ablation of this gene selectively removed the brake on NF-kappaB and c-jun activity in male T lymphocytes, resulting in higher production of interferon gamma and tumor necrosis factor (but not interleukin 17), and lower production of T helper (Th)2 cytokines. Upon induction of experimental autoimmune encephalomyelitis, male but not female PPARalpha(-/-) mice developed more severe clinical signs that were restricted to the acute phase of disease. These results suggest that males are less prone to develop Th1-mediated autoimmunity because they have higher T cell expression of PPARalpha