Hydrology, nutrient availability, and herbivory interacting to control ecosystem functions and services in created emergent freshwater wetlands

The loss of natural wetlands to anthropogenic development has warranted the creation of wetlands to mitigate the reduction of valuable ecosystem functions and services. However, the complex interactions between the main drivers of wetland community structure – hydrology, nutrient availability and herbivory – makes creation of fully functional wetland replacements challenging. In this study, we examined the interactions among these drivers, and their impacts on plant diversity and nitrogen removal in two created wetlands with different land use histories: A1N (previous gravel depository) and A3 (previous cattle pasture). We established paired plots protected from – and open to – large wetland grazers and compared vegetation abundance and diversity, soil characteristics, and soil respiration and potential denitrification in each wetland. At A1N, a permanently flooded emergent marsh with high observed grazer densities (predominantly waterfowl) and low nutrient availability, grazing significantly reduced plant growth and diversity. In contrast, at A3, a seasonally flooded wetland with lower grazer densities and high nutrient availability, grazing enhanced overall plant diversity and decreased invasive species cover. The effects of grazers varied seasonally and increased over time, eventually leading to a reduction in soil organic matter at both sites. In light of significant site differences, potential denitrification was limited by differential hydrology (very wet, anaerobic versus very dry, aerobic), nitrogen or carbon availability, and grazing (low or high levels) at both sites. These results suggest the possibility of long-term grazer-induced shifts in community composition and delivery of key ecosystem services in young, vulnerable created wetlands. To improve created wetland design and function, we suggest that the impact of prior land use on present nutrient availability must be considered, and sites must be heterogeneous in both physical and bathymetric structure, to provide conditions for diverse plant communities, both aerobic and anaerobic biogeochemical processes, and balanced habitat use by wetland grazers

Crystal Structure of Thermotoga maritima α-Glucosidase AglA Defines a New Clan of NAD+-dependent Glycosidases

Glycoside hydrolase family 4 represents an unusual group of glucosidases with a requirement for NAD(+), divalent metal cations, and reducing conditions. The family is also unique in its inclusion of both alpha- and beta-specific enzymes. The alpha-glucosidase A, AglA, from Thermotoga maritima is a typical glycoside hydrolase family 4 enzyme, requiring NAD(+) and Mn2+ as well as strongly reducing conditions for activity. Here we present the crystal structure of the protein complexed with NAD(+) and maltose, refined at a resolution of 1.9 Angstrom. The NAD(+) is bound to a typical Rossman fold NAD(+)-binding site, and the nicotinamide moiety is localized close to the maltose substrate. Within the active site the conserved Cys-174 and surrounding histidines are positioned to play a role in the hydrolysis reaction. The electron density maps indicate that Cys-174 is oxidized to a sulfinic acid. Most likely, the strongly reducing conditions are necessary to reduce the oxidized cysteine side chain. Notably, the canonical set of catalytic acidic residues common to other glucosidases is not present in the active site. This, combined with a high structural homology to NAD-dependent dehydrogenases, suggests an unusual and possibly unique mechanism of action for a glycoside-hydrolyzing enzyme

The pros and cons of using SDL for creation of distributed services

In a competitive market for the creation of complex distributed services, time to market, development cost, maintenance and flexibility are key issues. Optimizing the development process is very much a matter of optimizing the technologies used during service creation. This paper reports on the experience gained in the Service Creation projects SCREEN and TOSCA on use of the language SDL for efficient service creation

Supporting novel home network management interfaces with Openflow and NOX

The Homework project has examined redesign of existing home network infrastructures to better support the needs and requirements of actual home users. Integrating results from several ethnographic studies, we have designed and built a home networking platform providing detailed per-flow measurement and management capabilities supporting several novel management interfaces. This demo specifically shows these new visualization and control interfaces, and describes the broader benefits of taking an integrated view of the networking infrastructure, realised through our router's augmented measurement and control APIs. Aspects of this work have been published: the Homework Database in Internet Management (IM) 2011 and implications of the ethnographic results are to appear at the SIGCOMM W-MUST workshop 2011. Separate, more detailed expositions of the interface elements and system performance and implications are currently under submission at other venues. A partial code release is already available and we anticipate fuller public beta release by Q4 2011

Calculus Made Easy

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Cell wall chitosan is necessary for virulence in the opportunistic pathogen Cryptococcus neoformans

Cryptococcus neoformans is an opportunistic fungal pathogen that causes meningoencephalitis. Its cell wall is composed of glucans, proteins, chitin, and chitosan. Multiple genetic approaches have defined a chitosan-deficient syndrome that includes slow growth and decreased cell integrity. Here we demonstrate chitosan is necessary for virulence and persistence in the mammalian host

Inside Out: Detecting Learners' Confusion to Improve Interactive Digital Learning Environments

Confusion is an emotion that is likely to occur while learning complex information. This emotion can be beneficial to learners in that it can foster engagement, leading to deeper understanding. However, if learners fail to resolve confusion, its effect can be detrimental to learning. Such detrimental learning experiences are particularly concerning within digital learning environments (DLEs), where a teacher is not physically present to monitor learner engagement and adapt the learning experience accordingly. However, with better information about a learner's emotion and behavior, it is possible to improve the design of interactive DLEs (IDLEs) not only in promoting productive confusion but also in preventing overwhelming confusion. This article reviews different methodological approaches for detecting confusion, such as self-report and behavioral and physiological measures, and discusses their implications within the theoretical framework of a zone of optimal confusion. The specificities of several methodologies and their potential application in IDLEs are discussed

Home Network Management Policies: Putting the User in the Loop.

Home networks are becoming increasingly complex but existing management solutions are not simple to use since they are not tailored to the needs of typical home-users. In this paper we present a new approach to home network management that allows users to formulate quite sophisticated comic-strip policies using an attractive iPad application. The policies are based on the management wishes of home users elicited in a user study. Comic-strip policies are passed to a Policy engine running on a new Home Network Router designed to facilitate a variety of management tasks. We illustrate our approach via a number end-to-end experiments in an actual home deployment, using our prototype implementation. © 2012 IEEE