85 research outputs found
The Murine Reg3a Stimulated by Lactobacillus casei Promotes Intestinal Cell Proliferation and Inhibits the Multiplication of Porcine Diarrhea Causative Agent in vitro
Lactobacillus casei (L. casei), a normal resident of the gastrointestinal tract of mammals, has been extensively studied over the past few decades for its probiotic properties in clinical and animal models. Some studies have shown that some bacterium of Lactobacillus stimulate the production of antimicrobial peptides in intestinal cells to clear enteric pathogens, however, which antimicrobial peptides are produced by L. casei stimulation and its function are still not completely understood. In this study, we investigated the changes of antimicrobial peptides’ expression after intragastric administration of L. casei to mice. The bioinformatics analysis revealed there were nine genes strongly associated with up-regulated DEGs. But, of these, only the antimicrobial peptide mReg3a gene was continuously up-regulated, which was also confirmed by qRT-PCR. We found out the mReg3a expressed in engineering E.coli promoted cell proliferation and wound healing proved by CCK-8 assay and wound healing assay. Moreover, the tight junction proteins ZO-1 and E-cadherin in mReg3a treatment group were significantly higher than that in the control group under the final concentration of 0.2 mg/ml both in Porcine intestinal epithelial cells (IPEC-J2) and Mouse intestinal epithelial cells (IEC-6) (p < 0.05). Surprisingly, the recombinant mReg3a not only inhibited Enterotoxigenic Escherichia coli (ETEC), but also reduced the copy number of the piglet diarrheal viruses, porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and porcine rotavirus (PoRV), indicating the antimicrobial peptides mReg3a may be feed additives to resist the potential of the intestinal bacterial and viral diarrhea disease
Orbital parameters for an ELM white dwarf with a white dwarf companion: LAMOST J033847.06+413424.2
Double white dwarf systems are of great astrophysical importance in the field
of gravitational wave and Type Ia supernova. While the binary fraction of CO
core white dwarf is about a few percents, the extremely low mass white dwarfs
are all thought to be within binary systems. In this work, we report the
orbital solution of a double degenerate system: J033847.06+413424.24, an
extremely low mass He core white dwarf orbiting a CO core white dwarf. With
LAMOST and P200, time domain spectroscopic observations have been made and
spectral atmosphere parameters are estimated to be K and
log dex. Combining Gaia parallax, 3D extinction, and evolution
tracks, we estimate a radius of and a mass of
. With the 37 single exposure spectra, the radial velocities are
measured and the orbital parameters are estimated to be days,
km/s and km/s. The radial velocity based system
ephemeris is also provided. The light curves from several photometric surveys
show no orbital modulation. The orbital solution suggests that the invisible
companion has a minimum mass of about 0.60 and is
for an inclination of , indicating most probably a CO
core white dwarf. The system is expected to merge in about 1 Gyr. With present
period and distance ( pc) it can not irradiate strong enough
gravitational wave for LISA. More double degenerate systems are expected to be
discovered and parameterized as the LAMOST survey goes on.Comment: 12 pages, 11 figure
Isolation and Characterization of Cytotoxic, Aggregative Citrobacter freundii
Citrobacter freundii is an infrequent but established cause of diarrhea in humans. However, little is known of its genetic diversity and potential for virulence. We analyzed 26 isolates, including 12 from human diarrheal patients, 2 from human fecal samples of unknown diarrheal status, and 12 from animals, insects, and other sources. Pulsed field gel electrophoresis using XbaI allowed us to divide the 26 isolates into 20 pulse types, while multi-locus sequence typing using 7 housekeeping genes allowed us to divide the 26 isolates into 6 sequence types (STs) with the majority belonging to 4 STs. We analyzed adhesion and cytotoxicity to HEp-2 cells in these 26 strains. All were found to adhere to HEp-2 cells. One strain, CF74, which had been isolated from a goat, showed the strongest aggregative adhesion pattern. Lactate dehydrogenase (LDH) released from HEp-2 cells was evaluated as a measure of cytotoxicity, averaging 7.46%. Strain CF74 induced the highest level of LDH, 24.3%, and caused >50% cell rounding, detachment, and death. We named strain CF74 “cytotoxic and aggregative C. freundii.” Genome sequencing of CF74 revealed that it had acquired 7 genomic islands, including 2 fimbriae islands and a type VI secretion system island, all of which are potential virulence factors. Our results show that aggregative adherence and cytotoxicity play an important role in the pathogenesis of C. freundii
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Genome-Wide Analysis of DNA Methylation and Cigarette Smoking in a Chinese Population
Background: Smoking is a risk factor for many human diseases. DNA methylation has been related to smoking, but genome-wide methylation data for smoking in Chinese populations is limited. Objectives: We aimed to investigate epigenome-wide methylation in relation to smoking in a Chinese population. Methods: We measured the methylation levels at > 485,000 CpG sites (CpGs) in DNA from leukocytes using a methylation array and conducted a genome-wide meta-analysis of DNA methylation and smoking in a total of 596 Chinese participants. We further evaluated the associations of smoking-related CpGs with internal polycyclic aromatic hydrocarbon (PAH) biomarkers and their correlations with the expression of corresponding genes. Results: We identified 318 CpGs whose methylation levels were associated with smoking at a genome-wide significance level (false discovery rate < 0.05), among which 161 CpGs annotated to 123 genes were not associated with smoking in recent studies of Europeans and African Americans. Of these smoking-related CpGs, methylation levels at 80 CpGs showed significant correlations with the expression of corresponding genes (including RUNX3, IL6R, PTAFR, ANKRD11, CEP135 and CDH23), and methylation at 15 CpGs was significantly associated with urinary 2-hydroxynaphthalene, the most representative internal monohydroxy-PAH biomarker for smoking. Conclusion: We identified DNA methylation markers associated with smoking in a Chinese population, including some markers that were also correlated with gene expression. Exposure to naphthalene, a byproduct of tobacco smoke, may contribute to smoking-related methylation. Citation: Zhu X, Li J, Deng S, Yu K, Liu X, Deng Q, Sun H, Zhang X, He M, Guo H, Chen W, Yuan J, Zhang B, Kuang D, He X, Bai Y, Han X, Liu B, Li X, Yang L, Jiang H, Zhang Y, Hu J, Cheng L, Luo X, Mei W, Zhou Z, Sun S, Zhang L, Liu C, Guo Y, Zhang Z, Hu FB, Liang L, Wu T. 2016. Genome-wide analysis of DNA methylation and cigarette smoking in Chinese. Environ Health Perspect 124:966–973; http://dx.doi.org/10.1289/ehp.150983
Finite element analysis of individualized magnesium alloy meshes with different thickness
Objective To investigate the stress-strain distribution of magnesium alloy meshes with different thickness during bone healing by three-dimensional finite element analysis in order to provide a reference for the design of personalized magnesium alloy mesh. Methods After the cone-beam computed tomography (CBCT) data of a patient with tooth loss of C2-D2 and large alveolar defect were extracted, three-dimensional reconstruction was performed on the bone defect area. Three-dimensional finite element models of bone implant material and magnesium alloy meshes with different thickness were designed, and their stress-strain distributions were analyzed. Results The strain and stress showed a decreasing trend with the increase of the thickness of magnesium alloy mesh. During the process of bone healing, the stress of the bone implant material was gradually increased while the strain was gradually decreased. The strain of immature and mature bone was less than the strain at break. The stress was decreased first and then increased in the degradation of magnesium alloy mesh. Conclusion Magnesium alloy mesh of 0.3 and 0.4 mm thickness can only bear a small load, and can't meet the need of large area guided bone regeneration (GBR). The maximum equivalent stress of 0.5 mm-thickness magnesium alloy mesh is within the safe range, and the mesh has enough strength in the process of degradation, and can guide good bone healing and meet the clinical needs of GBR
An Optical Acoustic Detection System Based on Fabry Pérot Etalon Stability Structure
The optical acoustic detection system based on the Fabry Pérot Etalon (FPE) with high quality–factor (High Q) and stability structure is described and tested. The FPE contains two high–reflectivity Plano–Concave lenses, achieving high fineness and stability. The protective structure of the confocal stabilized FPE is composed of an invar tube, copper sheath, Bakelite sheath and aluminum housing to protect the sensor from the effects of ambient temperature and vibration. The audio signal is injected into the cavity through the sound hole located in the center of the cavity. Acoustic waves induce the vibration of the medium in the cavity, which leads to a simultaneous change in the FPE optical path and a shift of the interference spectrum. The acoustic detection system is built, and the frequency of the laser is locked on the resonant frequency points of the FPE by using phase modulation technology, so as to detect acoustic signals of different frequencies and amplitudes. In addition, the sensitivity of the proposed sensor exceeds 34.49 mV/Pa in the range of 20 Hz–20 kHz. A Signal-to-Noise Ratio (SNR) of 37 dB can be achieved at 20 Hz. Acoustic signal detection technology based on the FPE stability model is used to test the theoretical feasibility of the future high sensitivity Fabry Pérot Interferometric (FPI) acoustic sensors
A “Turn-On” Fluorescence Copper Biosensor Based on DNA Cleavage-Dependent Graphene Oxide-dsDNA-CdTe Quantum Dots Complex
A novel “turn-on” fluorescent copper biosensor is developed successfully based on the graphene oxide (GO)-dsDNA-CdTe quantum dots (QDs) complex via chemical crosslink method. The optical and structure properties of GO-dsDNA-CdTe QDs complex are studied by fluorescence (FL) spectra and transmission electron microscopy (TEM) in detail. It is demonstrated that the fluorescence quenching of CdTe QDs is a process of fluorescence resonance energy transfer (FRET) due to the essential surface and quenching properties of two-dimensional GO. Copper ions induce the catalytic reaction of DNA chain and irreversibly break at the cleavage site, which will cause the G-quadruplex formation, moreover further result in the CdTe QDs separated from GO and restored its fluorescence. Therefore, a significant recovery effect on the fluorescence of the GO-dsDNA-CdTe QDs complex is observed in the presence of copper ions. The fluorescence responses are concentration-dependent and can be well described by a linear equation. Compared with other metal ions, the sensor performs good selectivity for copper ions
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