Bem1p contributes to secretory pathway polarization through a direct interaction with Exo70p.

The exocyst serves to tether secretory vesicles to cortical sites specified by polarity determinants, in preparation for fusion with the plasma membrane. Although most exocyst components are brought to these sites by riding on secretory vesicles as they are actively transported along actin cables, Exo70p displays actin-independent localization to these sites, implying an interaction with a polarity determinant. Here we show that Exo70p directly and specifically binds to the polarity determinant scaffold protein Bem1p. The interaction involves multiple domains of both Exo70p and Bem1p. Mutations in Exo70p that disrupt its interaction with Bem1, without impairing its interactions with other known binding partners, lead to the loss of actin-independent localization. Synthetic genetic interactions confirm the importance of the Exo70p-Bem1p interaction, although there is some possible redundancy with Sec3p and Sec15p, other exocyst components that also interact with polarity determinants. Similar to Sec3p, the actin-independent localization of Exo70p requires a synergistic interaction with the phosphoinositide PI(4,5)P2

Investigation and Analysis on the CurrentSituation of Digitaltransformation of Xin yang Enterprises

ThepurposeofthisresearchonDigitaltransformationofXinyangenterprisesistograsptheneeds,speedandfollow-upguarantee ofXinyangenterprises'transformation under the planning of 2035's long-term goals. Based on the survey of more than 20 enterprises in Xinyang City, we adopted fi eld visits, telephone inquiries and other ways to creatively summarize the cognition of Xinyang enterprises on the application of digital technology; The digitalization of operation and production management implemented by Xinyang Enterprise; The organizational guarantee system of Xinyang Digital transformation. According to the research results, this paper puts forward the direction of Digital transformation of Xinyang government and enterprises, as well as the research results on the demand for talents of Xinyang Digital transformation, and puts forward corresponding countermeasures

Speciation of arsenic and selenium in rabbit using x-ray absorption spectroscopy

Chronic arsenic poisoning due to arsenic contamination of groundwater is a serious public health problem in Bangladesh and neighboring countries. Severe health effects associated with chronic exposure to arsenic include melanosis and several kinds of cancer. It is now generally agreed that the arsenic contamination of groundwater in Bangladesh is of geological origin. Arsenic naturally present in aquifers may be mobilized into drinking water by microbial action. The formation of a novel arsenic-selenium compound: seleno-bis (S-glutathionyl) arsinium ion, [(GS)2AsSe]-, and its subsequent excretion in rabbit bile has been demonstrated previously. This molecular basis for the in vivo antagonism between arsenic and selenium was discovered using X-ray absorption spectroscopy. There is growing evidence that, in Bangladeshi people who are suffering long term chronic lowlevel arsenic poisoning, this antagonism is causing a selenium deficiency. Administering selenium supplements might provide a simple but highly effective treatment of the Bangladeshi arsenic poisoning. In order to examine the disposition of [(GS)2AsSe]-, a set of rabbits were intravenously injected with selenite, arsenite or both. Whole blood, red blood cell and plasma samples were collected at different time intervals within 2hrs after injection and cecotrope samples 24hr after injection. Samples were examined using X-ray absorption spectroscopy and both arsenic and selenium K-near edge spectra were recorded. iii Speciation of arsenic and selenium will be discussed in this thesis. Results indicate that [(GS)2AsSe]- is formed in blood very rapidly after injection of both arsenite and selenite, and then is removed from blood stream within 2hrs post injection. Results also show that [(GS)2AsSe]- is assembled in red blood cells, with no [(GS)2AsSe]- detected in plasma samples. [(GS)2AsSe]- is also found in cecotrope samples after injection of both arsenite and selenite. The results of this study in rabbits will contribute to the understanding of chronic arsenic poisoning in humans

Construction of a Model Based Digital Twin Factory

In the digital factory system, physical factories, virtual factories, and digital twin service systems are three essential components. This article elaborates on the roles of the three in factory construction. The article utilizes m odel-based system engineering as the main idea to construct a digital twin service system, providing a basis for subsequent research

Selective Disassembly Research

This article transforms the disassembly graph model into a disassembly matrix model, and plans the path of selective disassembly by solving the matrix model.By conducting selective disassembly research on key components, a disassembly path is planned. When disassembling key parts, the goal is clear and unnecessary disassembly steps are omitted, greatly shortening the disassembly time and improving disassembly effi ciency.This article presents the results of this study through a case study, proving that the results can transform complex component connection diagrams into matrices, and then plan the disassembly path of key components