Jerry Lindsey v. Paul M. O\u27Conner

USDC for the Middle District of Pennsylvani

Digital data transition tracking loop improves data reception

Transition tracking loop eliminates drifts, leakages, and instabilities inherent in analog filters. Major components are the phase detector, loop filter, voltage-controlled oscillator and timing logic

“Other Knowers,” Other Growers: Gender-Conscious Farming and the“Alternative” Agriculture Movement

From the Washington University Senior Honors Thesis Abstracts (WUSHTA), Spring 2018. Published by the Office of Undergraduate Research. Joy Zalis Kiefer, Director of Undergraduate Research and Associate Dean in the College of Arts & Sciences; Lindsey Paunovich, Editor; Helen Human, Programs Manager and Assistant Dean in the College of Arts and Sciences Mentor: Heather O\u27Lear

Identifying Novel Therapeutic Targets in Myeloproliferative Neoplasms

From the Washington University Office of Undergraduate Research Digest (WUURD), Vol. 12, 05-01-2017. Published by the Office of Undergraduate Research. Joy Zalis Kiefer, Director of Undergraduate Research and Associate Dean in the College of Arts & Sciences; Lindsey Paunovich, Editor; Helen Human, Programs Manager and Assistant Dean in the College of Arts and Sciences. Mentor: Stephen O

The Effect of Negative Allosteric Modifiers on mGluR5 Activity

From the Washington University Office of Undergraduate Research Digest (WUURD), Vol. 12, 05-01-2017. Published by the Office of Undergraduate Research. Joy Zalis Kiefer, Director of Undergraduate Research and Associate Dean in the College of Arts & Sciences; Lindsey Paunovich, Editor; Helen Human, Programs Manager and Assistant Dean in the College of Arts and Sciences Mentor: Karen O\u27Malle

Characterizing Effects of Pevonedistat in Myeloproliferative Neoplasms

From the Washington University Office of Undergraduate Research Digest (WUURD), Vol. 13, 05-01-2018. Published by the Office of Undergraduate Research. Joy Zalis Kiefer, Director of Undergraduate Research and Associate Dean in the College of Arts & Sciences; Lindsey Paunovich, Editor; Helen Human, Programs Manager and Assistant Dean in the College of Arts and Sciences Mentor(s): Stephanie O

\u3ci\u3eAzospirillum brasilense\u3c/i\u3e Signaling via PilZ-containing Chemotaxis Receptors and Chemotaxis Protein Paralogs

Chemotaxis is the biased movement of bacteria in response to environmental stimuli. Chemotaxis is initiated with dedicated receptors that sense environmental signals, and these signals are transduced through a phosphorylation cascade that can influence flagellar rotation and swimming direction. Most knowledge of chemotaxis signal transduction comes from studies of Escherichia coli, which possesses a simple system consisting of five chemotaxis receptors and a single chemotactic signaling pathway. Unfortunately, E. coli is not representative of the diversity of chemotaxis signal transduction systems revealed by the analysis of completely sequenced bacterial genomes. Most sequenced bacterial genomes to date suggest most motile bacteria able of chemotaxis rely on at least two distinct chemotaxis signaling systems and a large number (\u3e20) chemotaxis receptors. This dissertation focuses on the plant growth promoting alphaproteobacterium Azospirillum brasilense as a representative model for chemotaxis. The genome of A. brasilense encodes four chemotaxis operons and fifty-one chemotaxis receptors. Here we show that chemotaxis paralogs encoded in two of these chemotaxis systems, Che1 and Che4, interact to produce two functional chemotaxis signaling arrays segregated by chemotaxis receptor lengths. One of these arrays contains a subclass of receptors that possess an additional Cterminal domain and comprise the PilZ-containing chemotaxis receptors. In bacteria, the Cterminal PilZ domain was shown to bind the secondary messenger cyclic diguanylate monophosphate which affects enzyme activity, gene expression and protein-protein interactions. Here we develop a novel real time tool (slide-in-chamber) and assay (root-in-pool) for monitoring the role of A. brasilense chemotaxis and PilZ-containing chemotaxis receptors (Tlp1 and Aer) in bacterial behavior and colonization of wheat rhizospheres. We found that chemotaxis is essential for A. brasilense to sense and subsequently colonize specific zones of wheat roots. A. brasilense exhibits an attractant response to the root hair and elongation zones of wheat and is repelled from the wheat root tip, and this response is dependent upon functional PilZ receptors Tlp1 and Aer. Furthermore, cyclic diguanylate monophosphate binding to Tlp1 and Aer is essential for bacterial response to roots. Taken together, these data reveal a new role for cyclic diguanylate monophosphate in modulating sensitivity to complex gradients

Portable medical status system

The hazards inherent in the Portable Medical Status System are identified, and the measures taken to reduce them to an acceptable level are described. Identification of these hazards is a prerequisite to use of the system on humans in the earth environment. One hazard which is insufficiently controlled and which is considered a constraint to use on humans is the level of current possible in the electrodes for the EEG (electroencephalograph) circuitry. It exceeds the maximum specified. A number of procedural and design recommendations for enhancement of safety are made

15.1 Frank Davey

Rampike Vol. 15 / No. 1 (Frank Davey issue): Frank Davey, Jeanette Lynes, Paul Hegedus, Robert Dassanowsky, Jürgen O. Olbrich, Brian Edwards, Penn Kemp, Louis Cabri, Nicole Markotic, Sandra Alland, Sarah Bonet, Anne Walker, George Bowering, Matthew Holmes, Stan Rogal, Tanis MacDonald, Lindsey Bannister, Paul Dutton, Michael and Linda Hutcheon, Charles Bernstein, Joyce Carol Oates, Gregory Betts, Darren Wershler-Henry, rob mclennan, Carl Peters, David King. Cover photo: Karl Jirgens featuring Frank Davey