Unique Features of Odorant-Binding Proteins of the Parasitoid Wasp Nasonia vitripennis Revealed by Genome Annotation and Comparative Analyses

Insects are the most diverse group of animals on the planet, comprising over 90% of all metazoan life forms, and have adapted to a wide diversity of ecosystems in nearly all environments. They have evolved highly sensitive chemical senses that are central to their interaction with their environment and to communication between individuals. Understanding the molecular bases of insect olfaction is therefore of great importance from both a basic and applied perspective. Odorant binding proteins (OBPs) are some of most abundant proteins found in insect olfactory organs, where they are the first component of the olfactory transduction cascade, carrying odorant molecules to the olfactory receptors. We carried out a search for OBPs in the genome of the parasitoid wasp Nasonia vitripennis and identified 90 sequences encoding putative OBPs. This is the largest OBP family so far reported in insects. We report unique features of the N. vitripennis OBPs, including the presence and evolutionary origin of a new subfamily of double-domain OBPs (consisting of two concatenated OBP domains), the loss of conserved cysteine residues and the expression of pseudogenes. This study also demonstrates the extremely dynamic evolution of the insect OBP family: (i) the number of different OBPs can vary greatly between species; (ii) the sequences are highly diverse, sometimes as a result of positive selection pressure with even the canonical cysteines being lost; (iii) new lineage specific domain arrangements can arise, such as the double domain OBP subfamily of wasps and mosquitoes.Rothamsted Research receives grant-aided support from the BBSRC of the UK. The authors thank Prof. David M. Shuker, University of Edinburgh, UK, who provided us with N. vitripennis. FGV was supported by a predoctoral fellowship SFRH/BD/22360/2005 from the ‘Fundac¸a˜o para a Cieˆncia e a Tecnologı´a’ (Portugal). This work was funded by grants BFU2007-62927 and BFU2010-15484 from the ‘Direccio´n General de Investigacio´n Cientı´fica y Te´cnica’ (Spain) to JR. JR was partially supported by ICREA Academia (Generalitat de Catalunya). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript

Manutenção do ser

Na seguinte dissertação apresenta-se uma proposta de estudo no campo da Perceção na Arquitetura, com a finalidade de obter resultantes sobre as relações que ambas exercem mutuamente no utilizador do espaço de forma a lograr diretrizes sobre como estes elementos podem melhorar as necessidades de uma faixa etária do homem, os idosos. Devido á realidade sentida na população portuguesa, em particular nas zonas do Interior, com o cada vez maior envelhecimento populacional e com o muitas vezes “esquecimento” que estes sofrem por parte da sociedade, este tema pareceu-me bastante importante e cativante de abordar. Para isto, torna-se necessário obter conhecimentos de como diferentes elementos arquitetónicos são analisados e processados pelo usuário do espaço e o que tais elementos são capazes de transmitir, estudando um pouco sobre o desenvolvimento de teorias da perceção humana. Assim tornar-se-á possível indicar quais os elementos arquitetónicos que afetam positivamente a faixa etária alvo do estudo, os idosos, de forma a seguidamente se conceptualizar e realizar um projeto de arquitetura que empregue os preceitos encontrados de forma correta para a estimulação dos experienciadores espaciais, transcendendo a Arquitetura, de uma mera característica de funcionalidade e estética a criadora de reacções e impulsos.The following dissertation presents a study proposal in the field of Perception in Architecture, with the purpose of obtaining results on the relationships that both exercise each other in the space user in order to achieve guidelines on how these elements can improve the needs of a band age of the man, the elderly. Due to the reality felt in the Portuguese population, in particular in the interior areas, with the increasing population aging and the often “forgetfulness” that they suffer from society, this topic seemed quite important and captivating to address. For this, it is necessary to obtain knowledge of how different architectural elements are analyzed and processed by the space user and what these elements are capable of transmitting, studying a little about the development of theories of human perception. Thus, it will be possible to indicate which architectural elements positively affect the target age group of the study, the elderly, in order to subsequently conceptualize and carry out an architectural project that employs the precepts found correctly for the stimulation of experimenters spatial, transcending architecture, from a mere characteristic of functionality and aesthetics to the creation of reactions and impulses

Mixed linear model for the prediction of infiltration rates in urban pervious concrete pavements in dense wooded parks

Abstract Clogging of porous concrete pavements is understood as a performance pathology within this peculiar concrete component. Albeit the clogging performance of porous concrete pavements is a sensitive topic in technical literature, most studies refer to laboratory investigations on concrete samples using different kinds of apparatus for the simulation of loss of permeability due to debris insertion on porous interstices. However, infiltration rate measurements are the basis for defining maintenance needs in actual pavements and shall be carried out periodically to measure and endorse infiltration rate adequacy as the kern of a clogging management protocol. This study dealt with a field experiment in a pervious concrete sidewalk surrounded by a wooded garden with tropical native and a variety of exotic trees. Over four years, eleven monitoring measurements were carried out using the ASTM C1701 infiltration ring test. The main contribution of this paper is a proposition of a mixed linear prediction model considering conditioned exponential distribution and a random normal intercept for the infiltration rate. From residual analysis and diagnosis plots, it can be seen that the proposed model presents a goodness of fit, allowing anticipation of infiltration rates reduction due to clogging by dense organic matter. Minimum infiltration rate is taken as 0.1 cm/s and such condition was achieved in a couple of months, awakening consciously to consider three to four intensive cleaning maintenance per year in dense wooded areas like public parks

Measurement of H₂O₂ within living drosophila during aging using a ratiometric mass spectrometry probe targeted to the mitochondrial matrix

Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) is central to mitochondrial oxidative damage and redox signaling, but its roles are poorly understood due to the difficulty of measuring mitochondrial H<sub>2</sub>O<sub>2</sub> in vivo. Here we report a ratiometric mass spectrometry probe approach to assess mitochondrial matrix H<sub>2</sub>O<sub>2</sub> levels in vivo. The probe, MitoB, comprises a triphenylphosphonium (TPP) cation driving its accumulation within mitochondria, conjugated to an arylboronic acid that reacts with H<sub>2</sub>O<sub>2</sub> to form a phenol, MitoP. Quantifying the MitoP/MitoB ratio by liquid chromatography-tandem mass spectrometry enabled measurement of a weighted average of mitochondrial H<sub>2</sub>O<sub>2</sub> that predominantly reports on thoracic muscle mitochondria within living flies. There was an increase in mitochondrial H<sub>2</sub>O<sub>2</sub> with age in flies, which was not coordinately altered by interventions that modulated life span. Our findings provide approaches to investigate mitochondrial ROS in vivo and suggest that while an increase in overall mitochondrial H<sub>2</sub>O<sub>2</sub> correlates with aging, it may not be causative

Priority questions for biodiversity conservation in the Mediterranean biome: Heterogeneous perspectives across continents and stakeholders

International audienceThe identification of research questions with high relevance for biodiversity conservation is an important step towards designing more effective policies and management actions, and to better allocate funding among alternative conservation options. However, the identification of priority questions may be influenced by regional differences in biodiversity threats and social contexts, and to variations in the perceptions and interests of different stakeholders. Here we describe the results of a prioritization exercise involving six types of stakeholders from the Mediterranean biome, which includes several biodiversity hotspots spread across five regions of the planet (Europe, Africa, North and South America, and Australia). We found great heterogeneity across regions and stakeholder types in the priority topics identified and disagreement among the priorities of research scientists and other stakeholders. However, governance, climate change, and public participation issues were key topics in most regions. We conclude that the identification of research priorities should be targeted in a way that integrates the spectrum of stakeholder interests, potential funding sources and regional needs, and that further development of interdisciplinary studies is required. The key questions identified here provide a basis to identify priorities for research funding aligned with biodiversity conservation needs in this biome

Physical characterixation and in vitro biological impact of highly aggregated antibodies separated into size-enriched populations by fluorescence-activated cell sorting

An IgG2 monoclonal antibody (mAb) solution was subjected to stirring, generating high concentrations of nanometer and subvisible particles, which were then successfully size enriched into different size bins by low speed centrifugation or a combination of gravitational sedimentation and Fluorescence-Activated Cell Sorting (FACS). The size-fractionated mAb particles were assessed for their ability to elicit the release of cytokines from a population of donor-derived human peripheral blood mononuclear cells (PBMC) at two phases of the immune response. Fractions enriched in nanometer-sized particles showed a lower response than those enriched in micron-sized particles in this assay. Particles of 5–10 μm in size displayed elevated cytokine release profiles compared to other size ranges. Stir-stressed mAb particles had amorphous morphology, contained protein with partially altered secondary structure, elevated surface hydrophobicity (compared to controls), and trace levels of elemental fluorine. FACS size-enriched the mAb particle samples, yet did not notably alter the overall morphology or composition of particles as measured by Microflow imaging, Transmission Electron Microscopy, and Scanning Electron Microscopy-Energy Dispersive X-ray Spectroscopy. The utility and limitations of FACS for size separation of mAb particles and potential of in-vitro PBMC studies to rank order the immunogenic potential of various types of mAb particles is discussed

Enhancing autophagy by redox regulation extends lifespan in <i>Drosophila</i>

Redox signalling is an important modulator of diverse biological pathways and processes, and operates through specific post-translational modification of redox-sensitive thiols on cysteine residues 1–4. Critically, redox signalling is distinct from irreversible oxidative damage and functions as a reversible ‘redox switch’ to regulate target proteins. H2O2 acts as the major effector of redox signalling, both directly and through intracellular thiol redox relays 5,6. Dysregulation of redox homeostasis has long been implicated in the pathophysiology of many age-related diseases, as well as in the ageing process itself, however the underlying mechanisms remain largely unclear 7,8. To study redox signalling by H2O2in vivo and explore its involvement in metabolic health and longevity, we used the fruit fly Drosophila as a model organism, with its tractable lifespan and strong evolutionary conservation with mammals 9. Here we report that inducing an endogenous redox-shift, by manipulating levels of the H2O2-degrading enzyme catalase, improves health and robustly extends lifespan in flies, independently of oxidative stress resistance and dietary restriction. We find that the catalase redox-shifted flies are acutely sensitive to starvation stress, which relies on autophagy as a vital survival mechanism. Importantly, we show that autophagy is essential for the lifespan extension of the catalase flies. Furthermore, using redox-inactive knock-in mutants of Atg4a, a major effector of autophagy, we show that the lifespan extension in response to catalase requires a key redox-regulatory cysteine residue, Cys102 in Atg4a. These findings demonstrate that redox regulation of autophagy can extend lifespan, confirming the importance of redox signalling in ageing and as a potential pro-longevity target.</jats:p