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Deep factorization for speech signal
Various informative factors mixed in speech signals, leading to great
difficulty when decoding any of the factors. An intuitive idea is to factorize
each speech frame into individual informative factors, though it turns out to
be highly difficult. Recently, we found that speaker traits, which were assumed
to be long-term distributional properties, are actually short-time patterns,
and can be learned by a carefully designed deep neural network (DNN). This
discovery motivated a cascade deep factorization (CDF) framework that will be
presented in this paper. The proposed framework infers speech factors in a
sequential way, where factors previously inferred are used as conditional
variables when inferring other factors. We will show that this approach can
effectively factorize speech signals, and using these factors, the original
speech spectrum can be recovered with a high accuracy. This factorization and
reconstruction approach provides potential values for many speech processing
tasks, e.g., speaker recognition and emotion recognition, as will be
demonstrated in the paper.Comment: Accepted by ICASSP 2018. arXiv admin note: substantial text overlap
with arXiv:1706.0177
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Endocytic recycling and vesicular transport systems mediate transcytosis of Leptospira interrogans across cell monolayer.
Many bacterial pathogens can cause septicemia and spread from the bloodstream into internal organs. During leptospirosis, individuals are infected by contact with Leptospira-containing animal urine-contaminated water. The spirochetes invade internal organs after septicemia to cause disease aggravation, but the mechanism of leptospiral excretion and spreading remains unknown. Here, we demonstrated that Leptospira interrogans entered human/mouse endothelial and epithelial cells and fibroblasts by caveolae/integrin-β1-PI3K/FAK-mediated microfilament-dependent endocytosis to form Leptospira (Lep)-vesicles that did not fuse with lysosomes. Lep-vesicles recruited Rab5/Rab11 and Sec/Exo-SNARE proteins in endocytic recycling and vesicular transport systems for intracellular transport and release by SNARE-complex/FAK-mediated microfilament/microtubule-dependent exocytosis. Both intracellular leptospires and infected cells maintained their viability. Leptospiral propagation was only observed in mouse fibroblasts. Our study revealed that L. interrogans utilizes endocytic recycling and vesicular transport systems for transcytosis across endothelial or epithelial barrier in blood vessels or renal tubules, which contributes to spreading in vivo and transmission of leptospirosis
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