Polymorphism at 93 nucleotide sites (and two indels) in partial sequences of the <i>gdh</i> gene (431 bp) in samples of <i>Giardia duodenalis</i> isolated from human faeces, animals and vegetables, from southern Brazil.

<p>The entire sequences can be found at GenBank with the accession numbers KJ741292-KJ741328. Taxon names indicate species (E, C, B or F = human; D = dog; H = vegetables), followed by identification numbers. Reference sequences are in bold.</p

Dendrograms of <i>Giardia duodenalis</i> based on nucleotide sequences of the <i>gdh</i> gene.

<p>Trees were constructed using the neighbour-joining (A) and maximum-likelihood (B) methods, implemented with MEGA 6. Numbers above or below branches indicate bootstrap values (%), estimated from 1,000 resamplings of the sequence data; bootstrap values <50% are not shown. Taxon names indicate species (E, C, B or F = human; D = dog; H = vegetables), followed by identification numbers. Reference sequences are in bold.</p

Detection of <i>G</i>. <i>duodenalis</i> in human faeces, dog faeces and vegetables using microscopy or the Merifluor Kit and assemblages found by PCR-RFLP and sequencing in Southern Brazil.

<p>ND: Not detected NR: Not performed</p><p>* Comparison of the ability to differentiate into assemblages by PCR-RFLP and sequencing (<i>gdh</i> gene) using Z test with p = 0.0008.</p><p>Detection of <i>G</i>. <i>duodenalis</i> in human faeces, dog faeces and vegetables using microscopy or the Merifluor Kit and assemblages found by PCR-RFLP and sequencing in Southern Brazil.</p