Raman scattering from phonons and magnons in RFe3)BO3)4

Inelastic light scattering spectra of several members of the RFe3(BO3)4 family reveal a cascade of phase transitions as a function of temperature, starting with a structural, weakly first order, phase transition followed by two magnetic phase transitions. Those consist of the ordering of the Fe-spin sublattice revealed by all the compound, and a subsequent spin-reorientational transition for GdFe3(BO3)4. The Raman data evidence a strong coupling between the lattice and magnetic degrees of freedom in these borates. The Fe-sublattice ordering leads to a strong suppression of the low energy magnetic scattering, and a multiple peaked two-magnon scattering continuum is observed. Evidence for short-range correlations is found in the `paramagnetic' phase by the observation of a broad magnetic continuum in the Raman data, which persists up to surprisingly high temperatures.Comment: 17 pages, 13 figure

An Adaptive Mutation in Enterococcus faecium LiaR Associated with Antimicrobial Peptide Resistance Mimics Phosphorylation and Stabilizes LiaR in an Activated State

The cyclic antimicrobial lipopeptide daptomycin (DAP) triggers the LiaFSR membrane stress response pathway in enterococci and many other Gram-positive organisms. LiaR is the response regulator that, upon phosphorylation, binds in a sequence-specific manner to DNA to regulate transcription in response to membrane stress. In clinical settings, non-susceptibility to DAP by Enterococcus faecium is correlated frequently with a mutation in LiaR of Trp73 to Cys (LiaRW73C). We have determined the structure of the activated E. faecium LiaR protein at 3.2 Å resolution and, in combination with solution studies, show that the activation of LiaR induces the formation of a LiaR dimer that increases LiaR affinity at least 40-fold for the extended regulatory regions upstream of the liaFSR and liaXYZ operons. In vitro, LiaRW73C induces phosphorylation-independent dimerization of LiaR and provides a biochemical basis for non-susceptibility to DAP by the upregulation of the LiaFSR regulon. A comparison of the E. faecalis LiaR, E. faecium LiaR, and the LiaR homolog from Staphylococcus aureus (VraR) and the mutations associated with DAP resistance suggests that physicochemical properties such as oligomerization state and DNA specificity, although tuned to the biology of each organism, share some features that could be targeted for new antimicrobials

Advances in Alfalfa Variety Development and Testing

Alfalfa (Medicago sativa) is historically the highest yielding, highest quality forage legume grown in Kentucky. It forms the basis of Kentucky\u27s cash hay enterprise and is an important component in dairy, horse, beef and sheep diets. Over 300,000 acres of alfalfa are grown annually in Kentucky, with state yields averaging between 3 and 4 tons per acre. The development and testing of alfalfa varieties is a dynamic process that impacts all Kentucky farmers. The Kentucky Alfalfa Variety Testing program was re-started in 1990 and is carried out through the efforts of several people, including Leonard Lauriault, Linda Brown (Western Kentucky University), Garry Lacefield, Paul Vincelli, and John Parr. Alfalfa varieties are being studied for yield in 6 plot studies over 3 locations (Lexington, Bowling Green, and Princeton). Other research being conducted include the effect of aphanomyces root rot on variety yield and persistence and the effect of variety on forage quality

Spectropolarimetry of SN 2011dh in M51: geometric insights on a Type IIb supernova progenitor and explosion

We present seven epochs of spectropolarimetry of the Type IIb supernova (SN) 2011dh in M51, spanning 86 days of its evolution. The first epoch was obtained 9 days after the explosion, when the photosphere was still in the depleted hydrogen layer of the stripped-envelope progenitor. Continuum polarization is securely detected at the level of P~0.5% through day 14 and appears to diminish by day 30, which is different from the prevailing trends suggested by studies of other core-collapse SNe. Time-variable modulations in P and position angle are detected across P-Cygni line features. H-alpha and HeI polarization peak after 30 days and exhibit position angles roughly aligned with the earlier continuum, while OI and CaII appear to be geometrically distinct. We discuss several possibilities to explain the evolution of the continuum and line polarization, including the potential effects of a tidally deformed progenitor star, aspherical radioactive heating by fast-rising plumes of Ni-56 from the core, oblique shock breakout, or scattering by circumstellar material. While these possibilities are plausible and guided by theoretical expectations, they are not unique solutions to the data. The construction of more detailed hydrodynamic and radiative-transfer models that incorporate complex aspherical geometries will be required to further elucidate the nature of the polarized radiation from SN 2011dh and other Type IIb supernovae.Comment: Post-proof edit. Accepted to MNRAS 2015 Aug 1

Multi-Epoch Spectropolarimetry for a Sample of Type IIn Supernovae: Persistent Asymmetry in Dusty Circumstellar Material

We present multi-epoch spectropolarimetry and spectra for a sample of 14 Type IIn supernovae (SNe IIn). We find that after correcting for likely interstellar polarization, SNe IIn commonly show intrinsic continuum polarization of 1--3% at the time of peak optical luminosity, although a few show weaker or negligible polarization. While some SNe IIn have even stronger polarization at early times, their polarization tends to drop smoothly over several hundred days after peak. We find a tendency for the intrinsic polarization to be stronger at bluer wavelengths, especially at early times. While polarization from an electron scattering region is expected to be grey, scattering of SN light by dusty circumstellar material (CSM) may induce such a wavelength-dependent polarization. For most SNe IIn, changes in polarization degree and wavelength dependence are not accompanied by changes in the position angle, requiring that asymmetric pre-SN mass loss had a persistent geometry. While 2--3% polarization is typical, about 30% of SNe IIn have very low or undetected polarization. Under the simplifying assumption that all SN IIn progenitors have axisymmetric CSM (i.e. disk/torus/bipolar), then the distribution of polarization values we observe is consistent with similarly asymmetric CSM seen from a distribution of random viewing angles. This asymmetry has very important implications for understanding the origin of pre-SN mass loss in SNe IIn, suggesting that it was shaped by binary interaction.Comment: 76 pages, 54 figures (13 in main text, 41 in appendix A

SN2012ab: A Peculiar Type IIn Supernova with Aspherical Circumstellar Material

We present photometry, spectra, and spectropolarimetry of supernova (SN) 2012ab, mostly obtained over the course of $\sim 300$ days after discovery. SN 2012ab was a Type IIn (SN IIn) event discovered near the nucleus of spiral galaxy 2MASXJ12224762+0536247. While its light curve resembles that of SN 1998S, its spectral evolution does not. We see indications of CSM interaction in the strong intermediate-width emission features, the high luminosity (peak at absolute magnitude $M=-19.5$), and the lack of broad absorption features in the spectrum. The H$\alpha$ emission undergoes a peculiar transition. At early times it shows a broad blue emission wing out to $-14{,}000$ km $\mathrm{s^{-1}}$ and a truncated red wing. Then at late times ($>$ 100$\,$days) it shows a truncated blue wing and a very broad red emission wing out to roughly $+20{,}000$ km $\mathrm{s^{-1}}$. This late-time broad red wing probably arises in the reverse shock. Spectra also show an asymmetric intermediate-width H$\alpha$ component with stronger emission on the red side at late times. The evolution of the asymmetric profiles requires a density structure in the distant CSM that is highly aspherical. Our spectropolarimetric data also suggest asphericity with a strong continuum polarization of $\sim 1-3$% and depolarization in the H$\alpha$ line, indicating asphericity in the CSM at a level comparable to that in other SNe IIn. We estimate a mass-loss rate of $\dot{M} = 0.050\, {\rm M}_{\odot}\,\mathrm{yr^{-1}}$ for $v_{\rm pre} = 100$$\,$km$\,$$\mathrm{s^{-1}}$ extending back at least 75$\,$yr prior to the SN. The strong departure from axisymmetry in the CSM of SN 2012ab may suggest that the progenitor was an eccentric binary system undergoing eruptive mass loss.Comment: 18 pages, 12 figure

Potent and selective inhibition of SH3 domains with dirhodium metalloinhibitors

Src-family kinases (SFKs) play important roles in human biology and are key drug targets as well. However, achieving selective inhibition of individual Src-family kinases is challenging due to the high similarity within the protein family. We describe rhodium(II) conjugates that deliver both potent and selective inhibition of Src-family SH3 domains. Rhodium(II) conjugates offer dramatic affinity enhancements due to interactions with specific and unique Lewis-basic histidine residues near the SH3 binding interface, allowing predictable, structure-guided inhibition of SH3 targets that are recalcitrant to traditional inhibitors. In one example, a simple metallopeptide binds the Lyn SH3 domain with 6 nM affinity and exhibits functional activation of Lyn kinase under biologically relevant concentrations (EC50 ∼ 200 nM)

Influence of a Cyclonic Eddy on Microheterotroph Biomass and Carbon Export in the Lee of Hawaii

[1] A multi‐platform sampling strategy was used to investigate carbon cycling in a cold‐core eddy that formed in the lee of Hawaii during September 2000. Microheterotroph biomass and 234Th‐derived carbon export rates within the eddy were 2 to 3 times higher than those observed for adjacent waters. If this eddy is representative of other cyclonic eddies that are frequently formed in the lee of Hawaii, then eddy activity may significantly enhance the areal efficiency of the biological pump and facilitate the transfer of organic carbon to organisms inhabiting the mesopelagic and abyssal‐benthic zones of this subtropical ecosystem