Identifikasi Molekuler Pasteurella Multocida Penyebab Pasteurellosis pada Babi di YOGYAKARTA

The purpose of this study was to determine the genetic variation of P.multocida in Yogyakarta with other overseas P. multocida strains. Samples were obtained from necropted intensive pig farming with clinically pneumonia symptoms, specific pulmonary lessions and then followed by histopathological examination and b iochemical characterization of the isolates. Then followed by histopathological examination and biochemical characterization of the isolates. Moleculartest performed by DNA extraction using the QIAGEN QIAamp DNA minikit, amplification of the 16S rRNA gene using forward primer 5' GGA GTG AAC TGC AGC TAA TAC C 3', and reverse primer 5' GTA GGT AAG CTT CGC GTT GTT G 3', electrophoresis, purification and sequencing. Macroscopic and histopathological examination results were analyzed descriptively. Sequencing r esults were analyzed by multiple alignment with other Pasteurella spp. taken from GenBank using the Clustal W software, subsequently analyzed using Neighbour Joining and Maximum Parsimony method that exist in program MEGA version 5.1 The results showed gen etic distance based on 765 nucleotides of 16S rRNA gene of P. multocida isolates from lungs of bronchopneumonia of swine in Yogyakarta at 0%. Filogram based on the nucleotide sequence showed a high similarity between P. multocida isolates from Yogyakarta a nd other isolates from USA, Germany, China, Europe, and Hungaria

Pengaruh Corpus Luteum dan Folikel Dominan terhadap Kualitas Morfologi Oosit Sapi Bali-Timor

Improvement of cattle production can be obtained by the application of feed and breeding technology. One of the breeding technologies that are rapidly growing is in vitro fertilization, which is part of In vitro embryo production The source of oocytes influence the success of in vitro embryo production The quality of the oocyte were determined by Ovarian follicular environment and followed the follicular growth that were characterized by the presence of a dominant follicle (DF) and corpus lutem (CL). The purpose of this study was to determine the influe nced of dominant follicles and corpus luteum on oocyte morphology ofBali Timor cattle, that were collected by aspiration method. Oocytes were obtained postmortem from the ovaries of Bali Timor cows slaughtered at an Oeba slaughterhouse Ovarian then class ified into 3 groups : I ) ovarian with CL without DF; (II) ovarian with DF, without CL; and (III) ovarian without both DF and CL. The results showed that the number of oocytes with good quality from group III (12.5%) was higher than group I (9.83%), but no significant differently (P> 0.05). The number of oocytes from group II that showed several layers of cells and has a cytoplasm (good quality) was very low

Fenotipe Pasteurella Multocida Penyebab Bronchopneumonia pada Babi di YOGYAKARTA

Pasteurella multocida is an important species that are suspected to cause bronchopneumonia in pigs. Prevalence of pneumonia pasteurellosis in slaughter house between 30-80%. The purpose of this study were determined the microorganisms that caused respiratory disorders (cough) or pneumonia in pigs in Yogyakarta area with morphological and biochemical tests. A number of 6 pigs were obtained from some pig farming in Yogyakarta with clinical symptoms of pneumonia were necropted, isolated and identified the cause of pneumonia, followed by histopathological examination of the isolates.The results showed that P. multocida is one of the main causes of bronchopneumonia in pigs in Yogyakarta with clinical symptoms of anorexia, dipsnoea, cough with serous to mucopurulent exudation. Pulmo showed grayish hepatization on dorsocranial, multifocal hemorrhagic and congestion. Histopathological changes found fibrinous bronchopneumonia and catharrhal bronchopneumonia

Pengaruh Corpus Luteum Dan Folikel Dominan Terhadap Kualitas Morfologi Oosit Sapi Bali-Timor

Improvement of cattle production can be obtained by the application of feed and breeding technology. One of the breeding technologies that are rapidly growing is in vitro fertilization, which is part of In vitro embryo production The source of oocytes influence the success of in vitro embryo production The quality of the oocyte were determined by Ovarian follicular environment and followed the follicular growth that were characterized by the presence of a dominant follicle (DF) and corpus lutem (CL). The purpose of this study was to determine the influe nced of dominant follicles and corpus luteum on oocyte morphology ofBali Timor cattle, that were collected by aspiration method. Oocytes were obtained postmortem from the ovaries of Bali Timor cows slaughtered at an Oeba slaughterhouse Ovarian then class ified into 3 groups : I ) ovarian with CL without DF; (II) ovarian with DF, without CL; and (III) ovarian without both DF and CL. The results showed that the number of oocytes with good quality from group III (12.5%) was higher than group I (9.83%), but no significant differently (P> 0.05). The number of oocytes from group II that showed several layers of cells and has a cytoplasm (good quality) was very low

Identifikasi Molekuler Pasteurella multocida Penyebab Pasteurellosis Pada Babi Di Yogyakarta

The purpose of this study was to determine the genetic variation of P.multocida in Yogyakarta with other overseas P. multocida strains. Samples were obtained from necropted intensive pig farming with clinically pneumonia symptoms, specific pulmonary lessions and then followed by histopathological examination and b iochemical characterization of the isolates. Then followed by histopathological examination and biochemical characterization of the isolates. Moleculartest performed by DNA extraction using the QIAGEN QIAamp DNA minikit, amplification of the 16S rRNA gene using forward primer 5' GGA GTG AAC TGC AGC TAA TAC C 3', and reverse primer 5' GTA GGT AAG CTT CGC GTT GTT G 3', electrophoresis, purification and sequencing. Macroscopic and histopathological examination results were analyzed descriptively. Sequencing r esults were analyzed by multiple alignment with other Pasteurella spp. taken from GenBank using the Clustal W software, subsequently analyzed using Neighbour Joining and Maximum Parsimony method that exist in program MEGA version 5.1 The results showed gen etic distance based on 765 nucleotides of 16S rRNA gene of P. multocida isolates from lungs of bronchopneumonia of swine in Yogyakarta at 0%. Filogram based on the nucleotide sequence showed a high similarity between P. multocida isolates from Yogyakarta a nd other isolates from USA, Germany, China, Europe, and Hungaria

Gambaran Histopatologi Beberapa Tipe Bronchopneumonia Pada Pulmo Babi Hasil Pemotongan Di Rph Oeba

The aim of this study was to determine the histopathologic changes of bronchopneumonia type in pigs of slaughtered pig from Oeba slaughterhouse associated with P. multocida infection. Samples of pulmo were found from pigs showing respiratoric symptoms, characterized as dyspnoea and presence of nasal exudates that are serous to mucopurulent, obtained from slaughtered pigs in RPH Oeba Kota Kupang. Samples showed pathognomonic lesions of gray hepatized than processed for histopathological studies. The result showed that varied macroscopic changes noted in pulmo samples from suspected infected pigs showed various types of lesions. Lessions observed then classified into two main lessions were acute and chronic. Acute lesions were characterized by edema and hyperemia which then confirmed with congestion and multifocal haemorrhages. Serous to seromucous exudates were observed intrabronchial and bronchioles. In chronic cases, the lesions showed cranioventral consolidation and gray hepatized with demarcated area beetwen consolidated and normal pulmonary area. Histologically, the types of bronchopneumonia categorized into bronchopneumonia suppurativa characterized by coagulative necrosis surrounded by cell infiltration and accumulation of fibrin intraalveolar. The fibrous connective tissue were also observed in the interstitaial space of alveolar around the necrotic area. Other samples showed non-suppurativa fibrinous bronchopneumonia with alveolar exudates dominated by mononuclear cells and accumulation of fibrin intra alveolar. Chronic bronchiolitis also seen with a mixture of mononuclear cells, neutrophils and macrophages that infiltrated into the lumen of the alveoli. A high number of goblet cells were observed in the bronchiolus epithelium, with the thickening of the fibrous tissue around the bronchioles. It can be concluded that there are two major types of lesions which are acute and chronic, with three types of bronchopnemonia namely bronchopneumonia suppurativa, non supuratival bronchopneumonia and chronic bronchiolitis

PENGARUH EKSTRAK DAUN C.ODORATA TERHADAP PROSES KESEMBUHAN LUKA INSISI PADA TIKUS SPRAGUE-DAWLEY

Chromolaena odorata (kirinyu/sufmuti) is a species of weed which utilized as traditional medicine especially for wound healing. This research was conducted to study the histological effect of C.odorata leaf extract to healing process of incision wound in Sprague-Dawley rats. A total of 45 males - 6 weeks of Sprague Dawley rats at 150-200g body weight were utilized in this study. Those rats were divided into five groups, basis ointment, Betadine 10%, C.odorata 5%, C.odorata 10%, and C.odorata 20%. All groups of rats were given a 3 cm long linear incision at dermal depth on the backs. The extract and medication were topically applied once a day for ten days. 3 rats of each group were euthanized in day 2, 6, and 10 after incision. Subsequently, skin tissue was obtained and fixated in 10% buffer formalin for Hematocixylin - Eosin staining. The measured variables were wound healing both macroscopical and microscopical figures from day 0 to day 10 after incision. Data were analyzed descriptively. Microscopic observation showed that various concentrations of C.odorata extracts affected wound healing process. C.odorata Extract is allegedly able to stimulate wound healing by increasing fibroblasts for re-epithelization

Fenotipe Pasteurella multocida Penyebab Bronchopneumonia Pada Babi Di Yogyakarta

Pasteurella multocida is an important species that are suspected to cause bronchopneumonia in pigs. Prevalence of pneumonia pasteurellosis in slaughter house between 30-80%. The purpose of this study were determined the microorganisms that caused respiratory disorders (cough) or pneumonia in pigs in Yogyakarta area with morphological and biochemical tests. A number of 6 pigs were obtained from some pig farming in Yogyakarta with clinical symptoms of pneumonia were necropted, isolated and identified the cause of pneumonia, followed by histopathological examination of the isolates.The results showed that P. multocida is one of the main causes of bronchopneumonia in pigs in Yogyakarta with clinical symptoms of anorexia, dipsnoea, cough with serous to mucopurulent exudation. Pulmo showed grayish hepatization on dorsocranial, multifocal hemorrhagic and congestion. Histopathological changes found fibrinous bronchopneumonia and catharrhal bronchopneumonia