Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site-0

<p><b>Copyright information:</b></p><p>Taken from "Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site"</p><p>http://www.biomedcentral.com/1472-6807/7/72</p><p>BMC Structural Biology 2007;7():72-72.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2194779.</p><p></p>s from the ring centroid to arginine 180 NH1 in the RTA-NMU complex are indicated

Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site-3

<p><b>Copyright information:</b></p><p>Taken from "Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site"</p><p>http://www.biomedcentral.com/1472-6807/7/72</p><p>BMC Structural Biology 2007;7():72-72.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2194779.</p><p></p> and NMU at 5°C (filled circles) and 30°C (filled triangles). () Titration with adenine (diamonds) and formamide (squares) at 25°C. The axis for adenine is in millimolar (top). A Scatchard plot for adenine binding is inset

Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site-2

<p><b>Copyright information:</b></p><p>Taken from "Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site"</p><p>http://www.biomedcentral.com/1472-6807/7/72</p><p>BMC Structural Biology 2007;7():72-72.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2194779.</p><p></p>ne. The position of Tyr80 in structure 1IFS is shown in cyan, and in 2P8N in yellow. () Closer view of the effect of binding adenine on the active site. Tyr80 in structure 1IFT (without adenine) is in green, 1IFS in cyan, and 2P8N in yellow. The 2mFo-DFc electron density of Tyr80 in 2P8N is contoured at 1.5σ. () RTA-NMU complex. A single water molecule is shown in light blue. () RTA-urea complex

Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site-5

<p><b>Copyright information:</b></p><p>Taken from "Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site"</p><p>http://www.biomedcentral.com/1472-6807/7/72</p><p>BMC Structural Biology 2007;7():72-72.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2194779.</p><p></p>ra in PBS buffer alone (solid line), 1 M urea (dashed line) and 2 mM adenine (dotted line). () Fluorescence in the presence of () 2 M formamide, () 1 M N-methylurea, () 1 M N, N-dimethylurea, () buffer alone, and () 1 M hydroxyurea, in order of decreasing maximal intensity

Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site-1

<p><b>Copyright information:</b></p><p>Taken from "Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site"</p><p>http://www.biomedcentral.com/1472-6807/7/72</p><p>BMC Structural Biology 2007;7():72-72.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2194779.</p><p></p>s from the ring centroid to arginine 180 NH1 in the RTA-NMU complex are indicated

Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site-4

<p><b>Copyright information:</b></p><p>Taken from "Fragment-based identification of determinants of conformational and spectroscopic change at the ricin active site"</p><p>http://www.biomedcentral.com/1472-6807/7/72</p><p>BMC Structural Biology 2007;7():72-72.</p><p>Published online 6 Nov 2007</p><p>PMCID:PMC2194779.</p><p></p