Cultivation of the New Zealand geoduck clam, Panopea zelandica

The geoduck Panopea zelandica has been signalled as a new emerging species for aquaculture in New Zealand. To pave the way for the establishment of a geoduck aquaculture industry, information on how to grow this species over its life cycle needs to be determined. The aim of this study was to identify conditions that optimize P. zelandica broodstock conditioning, fertilization, larval growth and metamorphosis, and juvenile and young adult growth. P. zelandica broodstock were conditioned within a combination of three water temperatures (7–8, 11–12, and 16–17°C) and three feeding rations (10,000, 50,000, and 100,000 cells mL-1 of Chaetoceros muelleri and Tisochrysis lutea, 1:1 cell counts) for 73 days. After conditioning, similar percent matured and dry condition index values were observed on geoducks among temperatures. However, significantly higher dry gonadosomatic indices (GSIdw) were recorded at 8 and 12°C. Although no difference was detected in the percentage of spawned individuals and connective tissue occupation indices, a higher percent of matured individuals was recorded when fed 10,000 and 50,000 cells mL-1. Glycogen, protein, and lipid analyses indicated that geoducks within all treatments achieved a positive energy balance, except for those in the treatments combining the highest temperature and lowest feeding ration. Comparisons of fatty acid profiles of animals among treatments and with the reference group (pond water conditioned) revealed that eicosapentaenoic (EPA, C20:5n-3), docosahexaenoic (DHA, C22:6n-3) and arachidonic (ARA, C20:4n-6) fatty acids were important contributors to gametogenic development for geoduck conditioning. ii The development of P. zelandica embryos at 15°C and 35 ppt and the optimal sperm:egg ratios for fertilization under hatchery conditions were investigated. Fertilization was conducted at sperm:egg ratios of: 50:1, 100:1, 500:1, 1000:1, and 10,000:1 with a sperm-egg contact time of 40 min. The optimal sperm:egg ratio was determined to be < 500:1 and the normal embryo yield at 3 and 18 h post-fertilization ranged from 83-96%. P. zelandica eggs (~ 80 μm diameter) developed the first and second polar bodies within 15 - 20 and 50 - 55 min post-fertilization, respectively. The blastula appeared at ~ 8 hpf, including the XR and XL cells and the presumptive shell field depression. Gastrulation occurred at 12 - 18 hpf with organic material shell apparent at the shell field depression. The mid-stage trochophore, which appeared at around 35 hpf had an apical plate with an apical tuft. The shell field spread to form the periostracum, which expanded and folded into right and left segments covering the late trochophore. The early D-stage veliger appeared at 45 hpf with the soft body being enclosed by two valves and the appearance of the velum. The physiological, morphological, and behavioral characteristics throughout the larval developmental process were determined for P. zelandica larvae, which were reared in a flow-through system at 17°C and 35 ppt. The initial veliger stocking densities ranged from 50 - 200 larvae mL-1 and geoduck larvae were fed continuously with Tisochrysis lutea and Chaetoceros calcitrans at residual algal levels of 20,000 to 80,000 cells mL-1 in three rearing batches. The larval development took 16 - 19 days from first D-veliger and metamorphosis occurred across a wide size range (300 - 375 μm shell length). The increase in shell length was linear over time and correlated with the deposition of striae in the prodissoconch II. The ingestion rate followed a power function with time and was closely correlated with the development of the alimentary system. Rearing iii with an initial stocking density of 100 larvae mL-1 and residual algal background of 20,000 cells mL-1 resulted in about 76% survival and 15 μm day-1 growth rate. The metamorphic induction of larval P. zelandica was tested with different neuroactive compounds. Two batches of competent hatchery-reared larvae were exposed to acetylcholine chloride, epinephrine hydrochloride, and excess potassium ions in the form of KCl and K2SO4 for 3 and 24 h. None of the tested chemicals increased the proportion of metamorphosed geoducks, and in some cases the chemical inhibited metamorphosis and caused significant mortality, despite having been used extensively with other species, such as mussels and oysters. The allometric coefficients (β) of respiration rate RR and clearance rate CR in P. zelandica were 0.73±0.03 and 0.62±0.07, respectively. P. zelandica juvenile and young adults were acutely thermally challanged at five different temperatures representative of potential farming conditions (8, 11, 15, 19, and 23°C). Their aerobic scope for activity and clearance rates were determined at all temperatures. Comparisons of aerobic scope for activity and clearance rates between size classes revealed that juvenile geoducks had a narrower thermal optimum than young adults (15 – 19°C versus 11 – 19°C, respectively). Temperatures higher than 19°C resulted in a reduction of aerobic scope for activity and clearance rate for both juvenile and young adults, which may lead to reduced performance and elevated mortality. P. zelandica juvenile and young adults were exposed to normoxia, mild hypoxia, and severe hypoxia. The respiration, aerobic scope, critical oxygen partial pressure (PcO2), and oxygen regulation capacity in two size classes of fed and starved animals were determined. The PcO2 was determined to be ~4 kPa for iv all geoduck groups. The respiration rates of fed small geoducks decreased significantly from normoxia (16.7 - ~21 kPa) to mild hypoxia (PcO2 - 16.7 kPa). Conversely, the respiration rates of starved geoducks from both size classes, and large fed geoducks were maintained at a constant level when exposed to the same change in oxygen concentration. However, all geoducks experienced decreased respiration rates during severe hypoxia (0 kPa - PcO2). In addition, overall oxyregulatory capacity, assessed using a regulation index, was affected by size rather than by nutritional stress. Large geoducks maintain oxygen consumption across an oxygen gradient more effectively than small geoducks. Also, the aerobic scope of small geoducks decreased significantly with declining PO2, while large geoducks maintained their aerobic scope under hypoxi

Extended network and algorithm finding maximal flows

Graph is a powerful mathematical tool applied in many fields as transportation, communication, informatics, economy, In ordinary graph the weights of edges and vertexes are considered independently where the length of a path is the sum of weights of the edges and the vertexes on this path. However, in many practical problems, weights at a vertex are not the same for all paths passing this vertex, but depend on coming and leaving edges. The paper develops a model of extended network that can be applied to modelling many practical problems more exactly and effectively. The main contribution of this paper is algorithm finding maximal flows on extended networks

Equivalence Between Vector Meson Dominance and Unitarised Chiral Perturbation Theory

It is explicitly shown that either the approximate solution of the integral equation for the inverse of the pion form facto,r or the result of the Pad$\text{\'e}$ approximant method of resumming the one loop Chiral Perturbation Theory (CPTH) are equivalent to the standard vector meson dominance (VMD) models, using the vector meson coupling to two pseudoscalars given by the KSRF relation. Inconsistencies between the one loop CPTH and its unitarised version (or the VMD model) are pointed out. The situation is better for the CPTH calculation of the scalar form factor and the related S-wave $\pi \pi$ scattering. The branching ratios of $\tau \to \pi^+ \pi^0 \nu$, $\tau \to K \pi \nu$, $\tau \to K^+ \eta \nu$ and \tau \to K^+ \bar{K^0} \nu\) using only two inputs as the \(\rho\) and \(K^*\) masses, or the two corresponding rms radii, agree with the experimental data. Using the same number of parameters, the corresponding one loop CPTH calculation cannot explain the \tau$ data

Analyzing the Sea Weather Effects to the Ship Maneuvering in Vietnam’s Sea from BinhThuan Province to Ca Mau Province Based on Fuzzy Control Method

Vietnam is located in the tropical monsoon climate, so there are many storms affecting the marine environment each year. However, Vietnam’s sea also has distinct characteristics due to the continental shelf factors, salinity, sea currents and viscosity water. In this paper, the sea weather effects to the ship in the sea area from BinhThuan province to Ca Mau province are analyzed. Specifically, wave, wind and current which are the three main factors affecting the safety of ship are thoroughly examined. Importantly, the survey parameters have been built from the actual operating environment. In addition, maintaining the stability of dynamic positioning system in Vietnam weather conditions is the main point of this study

Robust Subspace Tracking Algorithms in Signal Processing: A Brief Survey

Principal component analysis (PCA) and subspace estimation (SE) are popular data analysis tools and used in a wide range of applications. The main interest in PCA/SE is for dimensionality reduction and low-rank approximation purposes. The emergence of big data streams have led to several essential issues for performing PCA/SE. Among them are (i) the size of such data streams increases over time, (ii) the underlying models may be time-dependent, and (iii) problem of dealing with the uncertainty and incompleteness in data. A robust variant of PCA/SE for such data streams, namely robust online PCA or robust subspace tracking (RST), has been introduced as a good alternative. The main goal of this paper is to provide a brief survey on recent RST algorithms in signal processing. Particularly, we begin this survey by introducing the basic ideas of the RST problem. Then, different aspects of RST are reviewed with respect to different kinds of non-Gaussian noises and sparse constraints. Our own contributions on this topic are also highlighted

Three-Way Tensor Decompositions: A Generalized Minimum Noise Subspace Based Approach

Tensor decomposition has recently become a popular method of multi-dimensional data analysis in various applications. The main interest in tensor decomposition is for dimensionality reduction, approximation or subspace purposes. However, the emergence of “big data” now gives rise to increased computational complexity for performing tensor decomposition. In this paper, motivated by the advantages of the generalized minimum noise subspace (GMNS) method, recently proposed for array processing, we proposed two algorithms for principal subspace analysis (PSA) and two algorithms for tensor decomposition using parallel factor analysis (PARAFAC) and higher-order singular value decomposition (HOSVD). The proposed decomposition algorithms can preserve several desired properties of PARAFAC and HOSVD while substantially reducing the computational complexity. Performance comparisons of PSA and tensor decomposition of our proposed algorithms against the state-of-the-art ones were studied via numerical experiments. Experimental results indicated that the proposed algorithms are of practical values

Broadcast Gossip Based Distributed Hypothesis Testing in Wireless Sensor Networks

We consider the scenario that N sensors collaborate to observe a single event. The sensors are distributed and can only exchange messages through a network to reach a consensus about the observed event. In this paper, we propose a very robust and simple method using broadcast gossip algorithm to solve the distributed hypothesis testing problem. The simulation result shows that our method has good performance and is very energy efficient comparing to existing methods