Mechanistic role of a disease-associated genetic variant within the ADAM33 asthma susceptibility gene-0

<p><b>Copyright information:</b></p><p>Taken from "Mechanistic role of a disease-associated genetic variant within the ADAM33 asthma susceptibility gene"</p><p>http://www.biomedcentral.com/1471-2350/8/46</p><p>BMC Medical Genetics 2007;8():46-46.</p><p>Published online 17 Jul 2007</p><p>PMCID:PMC1955437.</p><p></p>lleles A and G respectively. Schematic defining the constructs generated to analyze the function of the two alleles A and G. The PCR generated fragments were cloned into the multiple cloning site at the NheI (N) and BglII (B) restriction sites of the pSEAP2 Basic vector

Mechanistic role of a disease-associated genetic variant within the ADAM33 asthma susceptibility gene-1

<p><b>Copyright information:</b></p><p>Taken from "Mechanistic role of a disease-associated genetic variant within the ADAM33 asthma susceptibility gene"</p><p>http://www.biomedcentral.com/1471-2350/8/46</p><p>BMC Medical Genetics 2007;8():46-46.</p><p>Published online 17 Jul 2007</p><p>PMCID:PMC1955437.</p><p></p> measured amount of beta galactosidase to obtain the normalized readings. All constructs that contained the Ar allele showed normalized readings similar to the Basic (-) control, which did not possess the SV40 promoter. The opposite result was observed for the constructs that contained the Pr allele. Pr allele constructs showed normalized readings similar to the Control (+), which possessed the SV40 promoter and the alkaline phosphatase reporter gene