1 research outputs found
New ACE-Inhibitory Peptides from Hemp Seed (<i>Cannabis sativa</i> L.) Proteins
A hemp
seed protein isolate, prepared from defatted hemp seed meals
by alkaline solubilization/acid precipitation, was subjected to extensive
chemical hydrolysis under acid conditions (6 M HCl). The resulting
hydrolysate was fractionated by semipreparative RP-HPLC, and the purified
fractions were tested as inhibitors of angiotensin converting enzyme
(ACE). Mono- and bidimensional NMR experiments and LC-MS analyses
led to the identification of four potentially bioactive peptides,
i.e. GVLY, IEE, LGV, and RVR. They were prepared by solid-phase synthesis,
and tested for ACE-inhibitory activity. The IC<sub>50</sub> values
were GVLY 16 ± 1.5 μM, LGV 145 ± 13 μM, and
RVR 526 ± 33 μM, confirming that hemp seed may be a valuable
source of hypotensive peptides