Collaborative interactions of heterogenous ribonucleoproteins contribute to transcriptional regulation of sterol metabolism in mice.

Heterogeneous nuclear ribonucleoproteins (hnRNPs) are a group of functionally versatile proteins that play critical roles in the biogenesis, cellular localization and transport of RNA. Here, we outline a role for hnRNPs in gene regulatory circuits controlling sterol homeostasis. Specifically, we find that tissue-selective loss of the conserved hnRNP RALY enriches for metabolic pathways. Liver-specific deletion of RALY alters hepatic lipid content and serum cholesterol level. In vivo interrogation of chromatin architecture and genome-wide RALY-binding pattern reveal insights into its cooperative interactions and mode of action in regulating cholesterogenesis. Interestingly, we find that RALY binds the promoter region of the master metabolic regulator Srebp2 and show that it directly interacts with coactivator Nuclear Transcription Factor Y (NFY) to influence cholesterogenic gene expression. Our work offers insights into mechanisms orchestrating selective promoter activation in metabolic control and a model by which hnRNPs can impact health and disease states

Obesity and pregnancy: possible ways to overcome complications and improve reproductive outcomes

Introduction. Obesity, due to its high prevalence and relevance, can be considered an epidemic of modern society. Obesity-related microcirculatory vascular disease and chronic inflammation with endothelial dysfunction are associated with adverse effects on reproductive potential. Sulodexide reduces the risk of hemostatic complications in patients planning pregnancy due to its polypotent effect, which is especially important in metabolic disorders and comorbidities. Therefore, it is promising to assess the effectiveness of integrated management of obese patients as part of pregnancy planning. Aim. To modify the complex treatment of obesity in patients planning pregnancy to reduce the risk of reproductive losses and gestational complications. Materials and methods. The prospective analysis included 92 patients with grade 1 obesity. According to the clinical protocols and guidelines of the Reproductive Health Professional Medical Association, 46 patients in the control group (group 1) were prescribed treatment to reduce the body mass index, including lifestyle correction, folic acid, and vitamin D. Patients in the comparison group (Group 2, n=46) also received endotheliotropic agent sulodexide. The treatment efficacy was analyzed based on the frequency of pregnancy, the change of laboratory markers of endothelial dysfunction, the incidence of gestational complications, and reproductive losses. Results. The study showed that in comparison group patients, the frequency of pregnancy (58.7% of cases in Group 1, 71.7% in Group 1), favorable course of the first trimester (39.1% in Group 1, 63.0% in Group 2), and delivery at term (30.4% in Group 1, 56.5% in Group 2) were higher compared to the control group. There was also a more significant decrease in the body mass index and endothelial dysfunction factors (homocysteine level in group 1 before treatment was 172.4 mol/L, and 141.8 mol/L after treatment; 191.7 mol/L before treatment and 91.4 mol/L after treatment in Group 2, respectively) in patients receiving complex treatment with sulodexide. Analysis of the pregnancy course showed a lower risk of gestational diabetes (33.3% in Group 1 and 13.8% in Group 2). Conclusion. Treatment of obesity in patients planning pregnancy requires a complex effect aimed at correcting lifestyle, reducing body weight, restoring the endothelial layer, and reducing the severity of chronic inflammation, thus improving reproductive outcomes and minimizing the risk of gestational complications

In vitro maturation for fertility preservation in patients with cancer: A review

According to the World Health Organization, the number of patients of reproductive age with cancer steadily increases. Therefore, improving their quality of life is one of the priority tasks of the medical community. It is known that more than 30% of women with cancer at the time of diagnosis have not yet given birth. Therefore, one of the relevant issues is developing and improving methods for preserving reproductive function. In vitro oocyte maturation is a promising technique of oncofertility, which is used as an alternative to traditional cycles of ovulation stimulation followed by the production of mature oocytes. This review aims to study the in vitro maturation procedure and analyze the literature data regarding its effectiveness and safety when used as a part of programs for preserving reproductive material in patients with cancer

Principles of fast-track surgery in the treatment of giant uterine fibroids: а clinical case

The article addresses the treatment of uterine fibroids, which is still relevant. According to numerous studies, about 70% of females under 50 have uterine fibroids, the most common condition among perimenopausal women. The prevalence of giant forms is not high and accounts for less than 5% of all cases, especially at early diagnosis. The surgical strategy in such cases is individual and depends on many factors: size, location of nodes, and women's reproduction plans. In these clinical cases, the management of patients with giant uterine fibroids is presented according to the multimodal principles of fast-track surgery in a modern gynecological hospital. We described the main approaches in diagnosis, preoperative preparation, surgery features, and subsequent recovery to reduce the complication rate, the duration of inpatient treatment, and postoperative rehabilitation in the treatment of giant uterine fibroids

SPA and health resort product of Jūrmala and its development opportunities

Bakalaura darba „Jūrmalas kūrorttūrisma produkts un tā attīstības iespējas” mērķis ir izstrādāt priekšlikumus Jūrmalas kūrorttūrisma produkta attīstībai, pamatojoties uz Jūrmalas kūrorta piedāvājuma un pieprasījuma analīzi. Bakalaura darbs sastāv no trim daļām. Pirmajā daļā tiek apskatīti kūrortu teorētiskie aspekti. Otrajā daļā, balstoties uz statistikas datiem, tiek izanalizēti Jūrmalas kūrorta produkta piedāvājums un pieprasījums, apskatīti veiktie mārketinga pasākumi, kas vērsti uz Jūrmalas kūrorta virzīšanu Latvijas un starptautiskajā tūrisma tirgū. Trešajā praktiskajā daļā ar anketēšanas palīdzību noteikta Latvijas un ārzemju tūristu apmierinātības pakāpe ar tūrisma pakalpojumiem Jūrmalas kūrortā, kā arī ar Jūrmalas kā kūrorta pilsētas tēlu. Darba beigās tika izstrādāti priekšlikumi kūrorttūrisma produkta attīstībai Jūrmalā. Bakalaura darba apjoms ir 107 lpp., darbā ir ietvertas 18 tabulas, 26 attēli un 4 pielikumi, izmantoti 56 literatūras avoti. Atslēgvārdi: Jūrmala, kūrorts, ārstniecība, kūrorta produkts, SPA.Aim of „SPA and health resort product of Jurmala and its development opportunities” diploma is to draw up suggestions for development of SPA and health resort product in Jurmala based on supply and demand analysis of Jurmala health resort. The diploma contains three main parts. Theoretical aspects of SPA and health resorts are described in first part. The supply and demand of Jurmala health resort based on statistical data analysis and also marketing mix directed to promotion of Jurmala heath resort in Latvian and international tourism market are examined and analyzed by author in second part. Third part is a research part where author defined level of Latvian and foreign tourists’ satisfaction with Jurmala’s health resort supply and also examined image of Jurmala as heath resort. Finalizing diploma suggestions were drawn up for Jurmala’s health resort product development. The diploma consists of 107 sheets, 18 tables, 26 pictures and 4 supplements; the reference list contains 56 entries. Key words: Jurmala, health resort, treatment, health resort product, SPA

Corporate Social Responsibility in the Framework of Quality Management

AbstractQuality management system (QMS) provide a framework for implementing corporate social responsibility (CSR) strategy in all management levels of organization, that create basis for establishment of sustainable development policy and related activities. Key for organizational excellence is the combination of focusing on quality in the process level and following the needs of stakeholders, giving the valuable contribution to the well-being of society.The aim of the research is to analyse concept of CSR in the context of organizational quality management system. This article presents the literature review on CSR definitions, organization excellence and sustainability concepts. The research showed that implementing employee-related CSR activities in quality management system of organization is the best alternative which helps to minimize staff rotation and improve the quality of processes and the overall performance of organization. Quality management system framework allows maintaining, monitoring and evaluating continuous effect and execution of CSR principles, and including CSR strategies in organizational policy

Flat bending shape stability of the beams with variable section width

The paper proposes a methodology for calculating lateral buckling of beams of variable rectangular cross section based on the energy approach. The technique is considered on the example of a cantilever beam of variable width with two sections under the action of a concentrated force. The twist angle function was set in the form of a trigonometric series. As a result, the problem is reduced to a generalized secular equation

Improving the energy method for calculating beams with a narrow rectangular section on the side buckling

We propose an improved version of the energy method in calculating rectangular beams for the stability of a flat bending shape. The essence of this variant of the method is to use the principle of the minimum total energy instead of the condition for the equality of the potential strain energy and the work of external forces. This version of the method makes it easy to obtain a numerical-analytical solution for any number of members of series. The solution of the problem for a pivotally supported beam is presented taking into account the vertical displacement of the load relative to the center of gravity

Antibacterial Properties of Fucoidans from the Brown Algae <i>Fucus vesiculosus</i> L. of the Barents Sea

Fucoidans, sulfated polysaccharides found in cell walls of brown algae, are considered as a promising antimicrobial component for various applications in medicine and the food industry. In this study, we compare the antibacterial properties of two fractions of fucoidan from the brown algae Fucus vesiculosus gathered in the littoral of the Barents Sea and sampled at different stages of purification. The crude fraction of fucoidan was isolated from algae by extraction with aqueous ethanol and sonication. The purified fraction was obtained by additional treatment of the crude fraction with a solution of calcium chloride. The structural features of both fractions were characterized in detail and their antibacterial effects against several Gram-positive and Gram-negative bacteria were compared by photometry, acridine orange staining assay, and atomic force microscopy. Fucoidan inhibited growth in all of the above microorganisms, showing a bacteriostatic effect with minimum inhibitory concentrations (MIC) in the range between 4 and 6 mg/mL, with E. coli being the most sensitive to both fractions. Changes in the chemical composition after treatment of the crude fraction with a solution of calcium chloride led to a decrease in the content of sulfates and uronic acids and diminished antibacterial activity