Nuclear actin and myosins in adenovirus infection

Adenovirus serotypes have been shown to cause drastic changes in nuclear organization, including the transcription machinery, during infection. This ability of adenovirus to subvert transcription in the host cell facilitates viral replication. Because nuclear actin and nuclear myosin I, myosin V and myosin VI have been implicated as direct regulators of transcription and important factors in the replication of other viruses, we sought to determine how nuclear actin and myosins are involved in adenovirus infection. We first confirmed reorganization of the host's transcription machinery to viral replication centers. We found that nuclear actin also reorganizes to sites of transcription through the intermediate but not the advanced late phase of viral infection. Furthermore, nuclear myosin I localized with nuclear actin and sites of transcription in viral replication centers. Intriguingly, nuclear myosins V and VI, which also reorganized to viral replication centers, exhibited different localization patterns, suggesting specialized roles for these nuclear myosins. Finally, we assessed the role of actin in adenovirus infection and found both cytoplasmic and nuclear actin likely play roles in adenovirus infection and replication. Together our data suggest the involvement of actin and multiple myosins in the nuclear replication and late viral gene expression of adenovirus.Fil: Fuchsova, Beata. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - La Plata. Instituto de Investigaciones Biotecnológicas. Instituto de Investigaciones Biotecnológicas ; Argentina. University of Illinois; Estados UnidosFil: Serebryannyy, Leonid A.. University of Illinois; Estados UnidosFil: De Lanerolle, Primal. University of Illinois; Estados Unido

Test It and They Might Come: Improving the Uptake of Digital Tools in Transparency and Accountability Initiatives

Information and communications technologies (ICTs) and data play an increasingly visible role in transparency and accountability initiatives (TAIs). There has been little research on how the selection of ICT tools influences the success of these initiatives. This article reports on research into TAI tool selection processes in South Africa and Kenya. Findings suggest that in many cases, tools are chosen with only limited testing of their appropriateness for the intended users in the intended contexts, despite widespread recognition among practitioners, funders and researchers that this carries significant efficiency and sustainability risks. We conclude by suggesting a strategy for increasing investment and effort in tool selection, in order to conserve overall project resources and minimise the risk of failure

Combining observations and numerical model results to improve estimates of hypoxic volume within the Chesapeake Bay, USA

© The Author(s), 2013. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Journal of Geophysical Research: Oceans 118 (2013): 4924–4944, doi:10.1002/jgrc.20331.The overall size of the “dead zone” within the main stem of the Chesapeake Bay and its tidal tributaries is quantified by the hypoxic volume (HV), the volume of water with dissolved oxygen (DO) less than 2 mg/L. To improve estimates of HV, DO was subsampled from the output of 3-D model hindcasts at times/locations matching the set of 2004–2005 stations monitored by the Chesapeake Bay Program. The resulting station profiles were interpolated to produce bay-wide estimates of HV in a manner consistent with nonsynoptic, cruise-based estimates. Interpolations of the same stations sampled synoptically, as well as multiple other combinations of station profiles, were examined in order to quantify uncertainties associated with interpolating HV from observed profiles. The potential uncertainty in summer HV estimates resulting from profiles being collected over 2 weeks rather than synoptically averaged ∼5 km3. This is larger than that due to sampling at discrete stations and interpolating/extrapolating to the entire Chesapeake Bay (2.4 km3). As a result, sampling fewer, selected stations over a shorter time period is likely to reduce uncertainties associated with interpolating HV from observed profiles. A function was derived that when applied to a subset of 13 stations, significantly improved estimates of HV. Finally, multiple metrics for quantifying bay-wide hypoxia were examined, and cumulative hypoxic volume was determined to be particularly useful, as a result of its insensitivity to temporal errors and climate change. A final product of this analysis is a nearly three-decade time series of improved estimates of HV for Chesapeake Bay.Funding for this study was provided by the IOOS COMT Program through NOAA grants NA10NOS0120063 and NA11NOS0120141. Additional funding was provided by NSF grant OCE-1061564

Nuclear actin: to polymerize or not to polymerize

The form and function of actin in the nucleus have been enigmatic for over 30 years. Recently actin has been assigned numerous functional roles in the nucleus, but its form remains a mystery. The intricate relationship between actin form and function in the cytoplasm implies that understanding the structural properties of nuclear actin is elementary to fully understanding its function. In this issue, McDonald et al. (p. 541) use fluorescence recovery after photobleaching (FRAP) to tackle the question of whether nuclear actin exists as monomers or polymers

A kidney transplant patient on cyclosporine therapy presenting with dural venous sinus thrombosis: a case report

Dural vein thrombosis is an important but rare cause of headache. Therapy with cyclosporine has been reported to result in thrombotic events. We report an unusual case of superior sagittal and transverse sinus thrombosis associated with cyclosporine therapy in a kidney transplant patient

What data do we want? Understanding demands for open data among civil society organisations in South Africa

Many governments, international agencies and civil society organisations (CSOs) support and promote open data. Most open government data initiatives have focused on supply – creating portals and publishing information. But much less attention has been given to demand – understanding data needs and nurturing engagement. This research examines the demand for open data in South Africa, and asks under what conditions meeting this demand might influence accountability. Recognising that not all open data projects are developed for accountability reasons, it also examines barriers to using government data for accountability processes. The research team identified and tested ‘use stories’ and ‘use cases’. How did a range of civil society groups with an established interest in holding local government accountable use – or feel that they could use – data in their work? The report identifies and highlights ten broad types of open data use, which they divided into two streams: ‘strategy and planning’ – in which CSOs used government data internally to guide their own actions; and ‘monitoring, mobilising and advocacy’ – in which CSOs undertake outward-facing activities.DFIDUSAIDSidaOmidyar Networ