Can an ecological scarcity method for Germany support robust decisions? – analysing the effect of uncertain target values on the impact assessment of energy generation technologies

Purpose Potentially contradictory indicators in Life Cycle Assessment cause ambiguity and thus uncertainty regarding the interpretation of results. The weighting-based ecological scarcity method (ESM) aims at reducing interpretation uncertainty by applying policy-based normative target values. However, the definition of these target values is uncertain due to different reasons such as questionable temporal representativeness. By means of an uncertainty analysis, this paper examines if ESMs are an appropriate approach to support robust decisions on multidimensional environmental impacts. Methods To assess the effect of uncertain target values (inputs) on environmental indicators (output), the ESM based Life Cycle Impact Assessment (LCIA) is combined with a Monte Carlo Analysis. The comprehensive uncertainty analysis includes the following steps: (1) sample generation, (2) output calculation and (3) results analysis and visualisation. (1) To generate a sample, moderate and strict limits for target values are derived from laws, directives or strategies. Random input parameters are drawn from a uniform distribution within those limits. (2) The sample is used to conduct several LCIAs leading to a distribution of total impact scores. (3) The results’ robustness is evaluated by means of the rank acceptability index to identify stable ranks for energy generation systems taken from ecoinvent v. 3.7.1. Results and discussion Applying moderate and strict target values in the ESM, results in substantial differences in the weighting sets. Even though the application of stricter target values changes the contribution of an environmental indicator to the total impact score the ranking of the energy generation systems varies only slightly. Moreover, the Monte Carlo Analysis reveals that displacement effects in ranks are not arbitrary: systems switch at most between ranks next to each other and most of the analysed systems dominate at least a single rank. Technologies with high shares of land use, global warming and air pollutants and particulate matter show a higher rank variance. Conclusions The weighting schemes, deduced from target values, provide a meaningful ranking of alternatives. At the same time, the results are not excessively sensitive to the uncertainties of the target values, i.e. the inherent uncertainty of the target values does not result in arbitrary outcomes, which is necessary to support robust decisions. The ESM is able to effectively facilitate decision making by making different environmental issues comparable

Essential Role of Lung Plasmacytoid Dendritic Cells in Preventing Asthmatic Reactions to Harmless Inhaled Antigen

Tolerance is the usual outcome of inhalation of harmless antigen, yet T helper (Th) type 2 cell sensitization to inhaled allergens induced by dendritic cells (DCs) is common in atopic asthma. Here, we show that both myeloid (m) and plasmacytoid (p) DCs take up inhaled antigen in the lung and present it in an immunogenic or tolerogenic form to draining node T cells. Strikingly, depletion of pDCs during inhalation of normally inert antigen led to immunoglobulin E sensitization, airway eosinophilia, goblet cell hyperplasia, and Th2 cell cytokine production, cardinal features of asthma. Furthermore, adoptive transfer of pDCs before sensitization prevented disease in a mouse asthma model. On a functional level, pDCs did not induce T cell division but suppressed the generation of effector T cells induced by mDCs. These studies show that pDCs provide intrinsic protection against inflammatory responses to harmless antigen. Therapies exploiting pDC function might be clinically effective in preventing the development of asthma

Cost-effective high-performance air-filled SIW antenna array for the global 5G 26 GHz and 28 GHz bands

A cost-effective, compact, and high-performance antenna element for beamforming applications in all fifth-generation (5G) New Radio bands in the [24.25-29.5] GHz spectrum is proposed in this letter. The novel antenna topology adopts a square patch, an edge-plated air-filled cavity, and an hourglass-shaped aperture-coupled feed to achieve a very high efficiency over a wide frequency band in a compact footprint (0.48 lambda(0) x 0.48 lambda(0)). Its compliance with standard printed circuit board (PCB) fabrication technology, without complex multilayer PCB stack, ensures low-cost fabrication. The antenna feedplane offers a platform for compact integration of active electronic circuitry. Two different modular 1 x 4 antenna arrays were realized to demonstrate its suitability for broadband multiantenna systems. Measurements of the fabricated antenna element and the antenna array prototypes revealed a-10 dB impedance bandwidth of 7.15 GHz (26.8%) and 8.2 GHz (30.83%), respectively. The stand-alone antenna features a stable peak gain of 7.4 +/- 0.6 dBi in the [24.25-29.5] GHz band and a measured total efficiency of at least 85%. The 1 x 4 array provides a peak gain of 10.1 +/- 0.7 dBi and enables grating-lobe-free beamsteering from -50 degrees to 50 degrees

The lung vascular filter as a site of immune induction for T cell responses to large embolic antigen

The bloodstream is an important route of dissemination of invading pathogens. Most of the small bloodborne pathogens, like bacteria or viruses, are filtered by the spleen or liver sinusoids and presented to the immune system by dendritic cells (DCs) that probe these filters for the presence of foreign antigen (Ag). However, larger pathogens, like helminths or infectious emboli, that exceed 20 µm are mostly trapped in the vasculature of the lung. To determine if Ag trapped here can be presented to cells of the immune system, we used a model of venous embolism of large particulate Ag (in the form of ovalbumin [OVA]-coated Sepharose beads) in the lung vascular bed. We found that large Ags were presented and cross-presented to CD4 and CD8 T cells in the mediastinal lymph nodes (LNs) but not in the spleen or liver-draining LNs. Dividing T cells returned to the lungs, and a short-lived infiltrate consisting of T cells and DCs formed around trapped Ag. This infiltrate was increased when the Toll-like receptor 4 was stimulated and full DC maturation was induced by CD40 triggering. Under these conditions, OVA-specific cytotoxic T lymphocyte responses, as well as humoral immunity, were induced. The T cell response to embolic Ag was severely reduced in mice depleted of CD11chi cells or Ly6C/G+ cells but restored upon adoptive transfer of Ly6Chi monocytes. We conclude that the lung vascular filter represents a largely unexplored site of immune induction that traps large bloodborne Ags for presentation by monocyte-derived DCs

Practitioner’s Section: Integrated Resource Efficiency Analysis for Reducing Climate Impacts in the Chemical Industry

Reducing greenhouse gas emissions of the material-intensive chemical industry requires an integrated analysis and optimization of the complex production systems including raw material and energy use, resulting costs and environmental and climate impacts. To meet this challenge, the research project InReff (Integrated Resource Efficiency Analysis for Reducing Climate Impacts in the Chemical Industry) has been established. It aims at the development of an IT-supported modeling and evaluation framework which is able to comprehensively address issues of resource efficiency and climate change within the chemical industry, e.g. the minimization of material and energy intensity and consequently greenhouse gas emissions, without compromising on production performance. The paper presents background information on resource efficiency and the research project, an ideal-typical decision model for resource efficiency analysis, the conceptual approach for an IT-based integration platform as well as the case study design at the industrial project partners’ sites. These first results are linked to future activities and further research questions are highlighted in the concluding section

Shape-Based Tracking Allows Functional Discrimination of Two Immune Cell Subsets Expressing the Same Fluorescent Tag in Mouse Lung Explant

Dendritic Cells (DC) represent a key lung immune cell population, which play a critical role in the antigen presenting process and initiation of the adaptive immune response. The study of DCs has largely benefited from the joint development of fluorescence microscopy and knock-in technology, leading to several mouse strains with constitutively labeled DC subsets. However, in the lung most transgenic mice do express fluorescent protein not only in DCs, but also in closely related cell lineages such as monocytes and macrophages. As an example, in the lungs of CX3CR1+/gfp mice the green fluorescent protein is expressed mostly by both CD11b conventional DCs and resident monocytes. Despite this non-specific staining, we show that a shape criterion can discriminate these two particular subsets. Implemented in a cell tracking code, this quantified criterion allows us to analyze the specific behavior of DCs under inflammatory conditions mediated by lipopolysaccharide on lung explants. Compared to monocytes, we show that DCs move slower and are more confined, while both populations do not have any chemotactism-associated movement. We could generalize from these results that DCs can be automatically discriminated from other round-shaped cells expressing the same fluorescent protein in various lung inflammation models

Life cycle assessment of biowaste treatment – Considering uncertainties in emission factors

The present paper provides an environmental assessment and ranking of different treatment options for the organic fraction of municipal solid waste (OFMSW), which is a biowaste that typically contains food- and green-waste. The main biochemical treatment systems for OFMSW are composting (by-product: fertilizer) and anaerobic digestion (by-products: biogas and digestate), which are often combined in practice. Total impact scores as evaluated by means of Life Cycle Assessment (LCA) with the Ecological Scarcity Method range from 8 to 31 eco-points for Germany (EPG) per kg treated OFMSW with “Global Warming” and “Main Air Pollutants” (such as NH3) as main environmental impacts. In spite of significant uncertainties in the available emission data, the subsequent application of a Monte Carlo Analysis provides a robust ranking of the treatment systems. The analysis reveals that the transportation distance is a crucial factor to increase the ecological benefit of an OFMSW treatment system. Consequently, decentral structures with short transportation distances (e.g. 17 km) as well as an optimal plant operation mode are more important to reduce emissions than the choice of the treatment system itself. Further research should focus on monitoring campaigns to generate better representative operational datasets for the different types of OFMSW treatment plants such as plug-flow or batch digestion systems as well as for the composting technologies

Ecological Scarcity Based Impact Assessment for a Decentralised Renewable Energy System

Increasing the share of renewable energies in electricity and heat generation is the cornerstone of a climate-friendly energy transition. However, as renewable technologies rely on diverse natural resources, the design of decarbonized energy systems inevitably leads to environmental trade-offs. This paper presents the case study of a comprehensive impact assessment for different future development scenarios of a decentralized renewable energy system in Germany. It applies an adapted ecological scarcity method (ESM) that improves decision-support by ranking the investigated scenarios and revealing their main environmental shortcomings: increased mineral resource use and pollutant emissions due to required technical infrastructure and a substantial increase in land use due to biomass combustion. Concerning the case study, the paper suggests extending the set of considered options, e.g., towards including imported wind energy. More generally, the findings underline the need for a comprehensive environmental assessment of renewable energy systems that integrate electricity supply with heating, cooling, and mobility. On a methodical level, the ESM turns out to be a transparent and well adaptable method to analyze environmental trade-offs from renewable energy supply. It currently suffers from missing quantitative targets that are democratically sufficiently legitimized. At the same time, it can provide a sound basis for an informed discussion on such targets

The miRFIB-Score: A Serological miRNA-Based Scoring Algorithm for the Diagnosis of Significant Liver Fibrosis

Background: The current diagnosis of early-stage liver fibrosis often relies on a serological or imaging-based evaluation of the stage of fibrosis, sometimes followed by an invasive liver biopsy procedure. Novel non-invasive experimental diagnostic tools are often based on markers of hepatocyte damage, or changes in liver stiffness and architecture, which are late-stage characteristics of fibrosis progression, making them unsuitable for the diagnosis of early-stage liver fibrosis. miRNAs control hepatic stellate cell (HSC) activation and are proposed as relevant diagnostic markers. Methods: We investigated the possibility of circulating miRNAs, which we found to be dysregulated upon HSC activation, to mark the presence of significant liver fibrosis (F ≥ 2) in patients with chronic alcohol abuse, chronic viral infection (HBV/HCV), and non-alcoholic fatty liver disease (NAFLD). Results: miRNA-profiling identified miRNA-451a, miRNA-142-5p, Let-7f-5p, and miRNA-378a-3p to be significantly dysregulated upon in vitro HSC activation, and to be highly enriched in their extracellular vesicles, suggesting their potential use as biomarkers. Analysis of the plasma of patients with significant liver fibrosis (F ≥ 2) and no or mild fibrosis (F = 0–1), using miRNA-122-5p and miRNA-29a-3p as positive control, found miRNA-451a, miRNA-142-5p, and Let-7f-5p, but not miRNA-378a-3p, able to distinguish between the two patient populations. Using logistic regression analysis, combining all five dysregulated circulating miRNAs, we created the miRFIB-score with a predictive value superior to the clinical scores Fibrosis-4 (Fib-4), aspartate aminotransferase/alanine aminotransferase (AST/ALT) ratio, and AST to platelet ratio index (APRI). The combination of the miRFIB-score with circulating PDGFRβ-levels further increased the predictive capacity for the diagnosis of significant liver fibrosis. Conclusions: The miRFIB- and miRFIBp-scores are accurate tools for the diagnosis of significant liver fibrosis in a heterogeneous patient population

Activation of Peroxisome Proliferator-Activated Receptor-γ in Dendritic Cells Inhibits the Development of Eosinophilic Airway Inflammation in a Mouse Model of Asthma

Peroxisome proliferator-activated receptors (PPARs) are activated by an array of polyunsaturated fatty acid derivatives, oxidized fatty acids, and phospholipids and are proposed to be important modulators of immune and inflammatory responses. Recently, we showed that activation of PPAR-γ alters the maturation process of dendritic cells (DCs), the most potent antigen-presenting cells. In the present report, we investigated the possibility that, by targeting DCs, PPAR-γ activation may be involved in the regulation of the pulmonary immune response to allergens. Using a model of sensitization, based on the intratracheal transfer of ovalbumin (OVA)-pulsed DCs, we show that rosiglitazone, a selective PPAR-γ agonist, reduces the proliferation of Ag-specific T cells in the draining mediastinal lymph nodes but, surprisingly enough, dramatically increases the production of the immunoregulatory cytokine interleukin (IL)-10 by T cells, as compared to control mice sensitized with OVA-pulsed DCs. After aerosol challenge, the recruitment of eosinophils in the bronchoalveolar lavage fluids was strongly reduced compared to control mice. Finally, T cells from the mediastinal lymph nodes produced higher amounts of IL-10 and interferon-γ. Inhibition of IL-10 activity with anti-IL-10R antibodies partly restored the inflammation. The specificity of the phenomenon was confirmed by treating OVA-pulsed DCs with ciglitazone, another PPAR-γ agonist, and by using GW9662, a PPAR-γ antagonist. Our data suggest that PPAR-γ activation prevents induction of Th2-dependent eosinophilic airway inflammation and might contribute to immune homeostasis in the lung