Special edition on oral infections and microbiology

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Predictors of oral health-related quality of life in patients following stroke

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Salivary Anionic Changes after Radiotherapy for Nasopharyngeal Carcinoma: A 1-Year Prospective Study

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SEM Image of Candida Albicans Biofilms on Plastic Coupons

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Oral health-related quality of life in patients with stroke: a randomized clinical trial of oral hygiene care during outpatient rehabilitation

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Effectiveness of a web-based health education program to promote oral hygiene care among stroke survivors: randomized controlled trial

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Survival of methicillin-resistant Staphylococcus aureus on toothbrushes stored under protective covers and sanitizers

Poster PresentationOBJECTIVES: To investigate the survival of methicillin-resistant Staphylococcus aureus on toothbrushes stored under commercially available antimicrobial and non-antimicrobial covers. METHODS: Standardized inocula of methicillin-resistant Staphylococcus aureus (MRSA) ATCC 700698 were prepared and adjusted to a 0.5 McFarland standard (~1.5x108 cfu/mL). A total of 45 toothbrush heads were contaminated with MRSA by submerging in 5mL of bacterial culture and vortexing for three minutes. Toothbrush heads were then stored for 24 hours under a thymol vapor-emitting toothbrush cover (Steripod), standard plastic cover with ventilation holes (Coghlan’s), moisture-absorbent cover (IntelliDent Toothbrush Shield), or ultraviolet light sanitizer (Docooler). Toothbrush heads were vortexed for 15s in 5mL of sterile 0.9% sodium chloride solution, and serial dilutions were performed prior to spiral plating on blood agar. A positive control was included in which toothbrush heads were immediately vortexed to remove bacteria following contamination with the inoculum. Colony-forming units (cfu) were enumerated after overnight aerobic incubation at 37oC. All assays were performed in triplicate. RESULTS: Significant reductions (p<0.001) in MRSA cfu were observed among toothbrush heads stored under all four experimental conditions, compared to the positive control. Survival of MRSA on toothbrush heads stored under standard plastic covers was significantly greater than those on toothbrush heads stored under the thymol vapor-emitting cover (p=0.006), moisture-absorbent cover (p=0.006), and UV sanitizer (p=0.011). Survival of MRSA (~4%) was equivocal on toothbrush heads stored under the latter three conditions. CONCLUSIONS: Survival of MRSA was significantly lowered on toothbrushes stored under the thymol vapor-emitting cover, moisture-absorbent cover, and UV sanitizer. None of the toothbrush covers were able to completely eradicate MRSA following a storage period of 24 hours

Antifungal activity of commercial mouthrinses against Candida albicans

Poster Session 22 - P1: no. 178OBJECTIVE: To investigate the effectiveness of delmopinol (Decapinol®), sodium chlorite (TheraBreath®), povidone iodine (Betadine®), and lactoferrin-lysozyme-lactoperoxidase (OralSevenTM) mouthrinses against C. albicans planktonic cells and biofilms. METHODS: Two-fold dilutions of the mouthrinse solutions were prepared (1:2, 1:4, 1:8, 1:16, 1:32, 1:64), and dispensed into 96 well microtitre plates. Standardized inocula of wild typ...link_to_OA_fulltex

Solasodine inhibits Candida albicans adhesion at sub-MIC level

Poster Session - Microbiology/Immunology-Oral Streptococci and Candida albicans: no. 492OBJECTIVES: To investigate the in vitro effects of solasodine on the adhesion of Candida albicans to buccal epithelial cells. METHODS: Buccal epithelial cells (BECs) were collected from healthy adults by gently rubbing the cheek mucosa with sterile swabs, and washed twice in phosphate-buffered saline. Equal volumes of BECs (105 cells/mL) and wild type C. albicans SC5314 (107 cells/mL) were incubated at 37oC for 1 h with gentle agitation in the absence or presence of sub-MIC level (60 μM) of solasodine, and filtered through 12 μm pore polycarbonate filters. Unattached cells were washed away, and the BECs were air-dried and Gram’s stained. Attached fungal cells were counted under light microscope in 50 BECs. qRT-PCR was used to evaluate variations in expression of adhesion-related genes in solasodine-treated fungal cells. Total RNA was extracted using SV Total RNA Isolation System and reverse transcribed with Superscript II. Gene expression was determined by Fast SYBR Green Master Mix with gene-specific primers, and reactions were run: 95oC incubation for 20 s, followed by 40 cycles of 95oC incubation for 1 s and 60oC for 20 s. EFB1 was used for reference. All experiments were performed in triplicate in three different occasions. Student’s t test was used, and a P-value of <0.05 was considered statistically significant. RESULTS: Solasodine inhibited C. albicans adhesion to BECs by ~52% (P < 0.05). qRT-PCR analysis showed that while the gene expression of BCR1 was not affected, the gene expression of ALS3, ECE1, and HWP1 was reduced. However, expression of ALS1 was promoted. CONCLUSIONS: The collective data of the present study suggest that solasodine suppresses C. albicans adhesion to the BECs via downregulation of the expression of adhesion-related genes ALS3, ECE1, and HWP1. This provides insights for further investigation of this compound with respect to its antifungal effectiveness in clinical intervention studies. THIS ABSTRACT IS BASED ON RESEARCH THAT WAS FUNDED ENTIRELY OR PARTIALLY BY AN OUTSIDE SOURCE: Health and Medical Research Fund, 1110099