Profile of isoflavones in genetically modified soy.

Isoflavones are important compounds in soy with functional claims. Their concentrations are influenced by growing conditions and genetics. With the development of genetically modified varieties is crucial to know the levels of these substances in new seeds. Analyzed in six transgenic soybean cultivars (BRS-FavoritaRR, BRS-ValiosaRR, BRSMG-850GRR, BRSMG-811CRR, BRSMG-750SRR and BRSMG-740SRR) isoflavones (genistein, daidzein, and glycitein in their four chemical forms) by HPLC. The results were compared in mg/100g of dry and defatted samples. Statistical analysis was performed using Turkey test (p <0.05). Observed in the raw grain, the absence of detectable amounts of the isoflavones with acetyl radical, as well as the aglucone glycitein. The literature describes genistein aglycone as the most active and BRS-ValiosaRR showed a lower level and BRS-FavoritaRR the largest, not differing significantly from this variety BRSMG-750SRR for this analyte. The aglycone forms of daidzein is second in biological activity and varieties BRSMG-811CRR and BRS-ValiosaRR had lower grades and BRSMG 750SRR-and BRS-FavoritaRR the largest. The total isoflavones did not differ significantly between BRSMG-811CRR (413.86d) and BRSMG740-SRR (435.72d) but between these and BRSMG-750SRR (491.94c). The BRS-ValiosaRR (533.73b) was similar to BRSMG-850GRR (549.08b) but differed BRS-FavoritaRR (591.29a) that higher concentration. The BRS-FavoritaRR generally show the best performance while BRSMG-810CRR the worst. This knowledge is relevant in terms of both technology development (seed richest) and showing the quantity of food being consumed (considering the content of isoflavones)

A 32-channel photon counting module with embedded auto/cross-correlators for real-time parallel fluorescence correlation spectroscopy

Fluorescence correlation spectroscopy (FCS) is a well-established technique to study binding interactions or the diffusion of fluorescently labeled biomolecules in vitro and in vivo. Fast FCS experiments require parallel data acquisition and analysis which can be achieved by exploiting a multi-channel Single Photon Avalanche Diode (SPAD) array and a corresponding multi-input correlator. This paper reports a 32-channel FPGA based correlator able to perform 32 auto/cross-correlations simultaneously over a lag-time ranging from 10 ns up to 150 ms. The correlator is included in a 32 × 1 SPAD array module, providing a compact and flexible instrument for high throughput FCS experiments. However, some inherent features of SPAD arrays, namely afterpulsing and optical crosstalk effects, may introduce distortions in the measurement of auto- and cross-correlation functions. We investigated these limitations to assess their impact on the module and evaluate possible workarounds

Comparison between different rotary mowing systems: Testing a new method to calculate turfgrass mowing quality

Poor quality in turfgrass mowing is highlighted by the shredded leaf tips with necrotic tissues that give an unsightly brownish colour to the turf and may also lead to turf disease. Mowing quality is also typically assessed by visual rating, thus the score depends on the person doing the assessment. To make the evaluation of mowing quality not subjective, an innovative method was developed. The aim of the trial was to examine the effects of different mowing systems and two different nitrogen rates (100 and 200 kg ha−1 ) on two turfgrass species in order to test the new mowing quality calculation. Three different mowing systems were used: a battery-powered rotary mower set at 3000 rpm and 5000 rpm respectively and a gasoline-powered rotary mower set at full throttle. The battery-powered mower at low blade rpm produced a poorer mowing quality and turf quality than the gasoline-powered mower and battery-powered mower at high rpm, which produced a similar mowing quality and turf quality. Leaf tip damage level values showed a significant correlation with the results of the visual mowing quality assessment. Lower leaf tip damage level values (slightly above 1) corresponded to higher visual mowing quality scores (around 8)

Energetic aspects of turfgrass mowing: comparison of different rotary mowing systems.

Turfgrass mowing is one of the most important operations concerning turfgrass maintenance. Over time, dierent mowing machines have been developed, such as reel mowers, rotary mowers, and flail mowers. Rotary mowers have become the most widespread mowers for their great versatility and easy maintenance. Modern rotary mowers can be equipped with battery-powered electric motors and precise settings, such as blade rpm. The aim of this trial was to evaluate the dierences in power consumption of a gasoline-powered rotary mower and a battery-powered rotary mower. Each mower worked on two dierent turfgrass species (bermudagrass and tall fescue) fertilized with two dierent nitrogen rates (100 and 200 kg ha1). The battery-powered mower was set at its lowest and highest blade rpm value, while the gasoline-powered mower was set at full throttle. From the data acquired, it was possible to see that the gasoline-powered mower had a much higher primary energy requirement, independent of the turf species. Moreover, comparing the electricity consumption of the battery-powered mower over time, it was possible to see that the power consumption varied according to the growth rate of both turf species. These results show that there is a partial waste of energy when using a gasoline-powered mower compared to a battery-powered mower

Bottom Production

We review the prospects for bottom production physics at the LHC.Comment: 74 pages, Latex, 71 figures, to appear in the Report of the ``1999 CERN Workshop on SM physics (and more) at the LHC'', P. Nason, G. Ridolfi, O. Schneider G.F. Tartarelli, P. Vikas (conveners

Heme oxygenase-1 in the forefront of a multi-molecular network that governs cell–cell contacts and filopodia-induced zippering in prostate cancer

Prostate cancer (PCa) cells display abnormal expression of cytoskeletal proteins resulting in an augmented capacity to resist chemotherapy and colonize distant organs. We have previously shown that heme oxygenase 1 (HO-1) is implicated in cell morphology regulation in PCa. Here, through a multi 'omics' approach we define the HO-1 interactome in PCa, identifying HO-1 molecular partners associated with the integrity of the cellular cytoskeleton. The bioinformatics screening for these cytoskeletal-related partners reveal that they are highly misregulated in prostate adenocarcinoma compared with normal prostate tissue. Under HO-1 induction, PCa cells present reduced frequency in migration events, trajectory and cell velocity and, a significant higher proportion of filopodia-like protrusions favoring zippering among neighboring cells. Moreover forced expression of HO-1 was also capable of altering cell protrusions in transwell co-culture systems of PCa cells with MC3T3 cells (pre-osteoblastic cell line). Accordingly, these effects were reversed under siHO. Transcriptomics profiling evidenced significant modulation of key markers related to cell adhesion and cell–cell communication under HO-1 induction. The integration from our omics-based research provides a four molecular pathway foundation (ANXA2/HMGA1/POU3F1; NFRSF13/GSN; TMOD3/RAI14/VWF; and PLAT/PLAU) behind HO-1 regulation of tumor cytoskeletal cell compartments. The complementary proteomics and transcriptomics approaches presented here promise to move us closer to unravel the molecular framework underpinning HO-1 involvement in the modulation of cytoskeleton pathways, pushing toward a less aggressive phenotype in PCa

Hepatic encephalopathy increases the risk for mortality and hospital readmission in decompensated cirrhotic patients: a prospective multicenter study

Introduction: Hepatic encephalopathy (HE) affects the survival and quality of life of patients with cirrhosis. However, longitudinal data on the clinical course after hospitalization for HE are lacking. The aim was to estimate mortality and risk for hospital readmission of cirrhotic patients hospitalized for HE. Methods: We prospectively enrolled 112 consecutive cirrhotic patients hospitalized for HE (HE group) at 25 Italian referral centers. A cohort of 256 patients hospitalized for decompensated cirrhosis without HE served as controls (no HE group). After hospitalization for HE, patients were followed-up for 12 months until death or liver transplant (LT). Results: During follow-up, 34 patients (30.4%) died and 15 patients (13.4%) underwent LT in the HE group, while 60 patients (23.4%) died and 50 patients (19.5%) underwent LT in the no HE group. In the whole cohort, age (HR 1.03, 95% CI 1.01–1.06), HE (HR 1.67, 95% CI 1.08–2.56), ascites (HR 2.56, 95% CI 1.55–4.23), and sodium levels (HR 0.94, 95% CI 0.90–0.99) were significant risk factors for mortality. In the HE group, ascites (HR 5.07, 95% CI 1.39–18.49) and BMI (HR 0.86, 95% CI 0.75–0.98) were risk factors for mortality, and HE recurrence was the first cause of hospital readmission. Conclusion: In patients hospitalized for decompensated cirrhosis, HE is an independent risk factor for mortality and the most common cause of hospital readmission compared with other decompensation events. Patients hospitalized for HE should be evaluated as candidates for LT

Development of ISB 1442, a CD38 and CD47 bispecific biparatopic antibody innate cell modulator for the treatment of multiple myeloma

Antibody engineering can tailor the design and activities of therapeutic antibodies for better efficiency or other advantageous clinical properties. Here we report the development of ISB 1442, a fully human bispecific antibody designed to re-establish synthetic immunity in CD38+ hematological malignancies. ISB 1442 consists of two anti-CD38 arms targeting two distinct epitopes that preferentially drive binding to tumor cells and enable avidity-induced blocking of proximal CD47 receptors on the same cell while preventing on-target off-tumor binding on healthy cells. The Fc portion of ISB 1442 is engineered to enhance complement dependent cytotoxicity, antibody dependent cell cytotoxicity and antibody dependent cell phagocytosis. ISB 1442 thus represents a CD47-BsAb combining biparatopic targeting of a tumor associated antigen with engineered enhancement of antibody effector function to overcome potential resistance mechanisms that hamper treatment of myeloma with monospecific anti-CD38 antibodies. ISB 1442 is currently in a Phase I clinical trial in relapsed refractory multiple myeloma