A higher Angiogenin expression is associated with a non-nuclear Maspin location in laryngeal carcinoma

Objectives. In numerous malignancies, angiogenin (ANG) and Maspin are important proangiogenic and antiangiogenic regulators, respectively. The aim of this study was to identify potential relationships between the biological roles of these two proteins in laryngeal squamous cell carcinoma (LSCC). Methods. Immunohistochemical staining for ANG and Maspin was performed on specimens from 76 consecutive LSCC patients treated with surgery alone, considering the subcellular pattern of Maspin expression. Univariate and multivariate statistical models were used for prognostic purposes. Results. On univariate analysis, a different level of ANG expression was seen for patients stratified by subcellular Maspin expression pattern: the mean ANG expression was higher in cases with a nonnuclear MASPIN expression than in those with a nuclear pattern (P=0.002). Disease-free survival (DFS; in months) differed significantly when patients were stratified by N stage (P=0.01). Patients whose Maspin expression was nonnuclear (i.e., it was cytoplasmic or there was none) had a significantly higher recurrence rate (P<0.001), and shorter DFS (P=0.01) than those with a nuclear Maspin pattern. The mean ANG expression was significantly higher in cases with loco-regional recurrent disease (P=0.007); and patients with an ANG expression 655.0% had a significantly shorter DFS than those with an ANG expression <5.0% (P=0.007). On multivariate analysis, ANG expression 655.0% was a significant, independent, negative prognostic factor in terms of DFS (P=0.041). Conclusion. Our results support the hypothesis that a higher ANG expression is associated with a nonnuclear Maspin expression pattern in patients with LSCC. Further studies are needed to clarify the relationship between the ANG and Maspin pathways, and their potential diagnostic and therapeutic role in LSCC

Local chiral interactions and magnetic structure of few-nucleon systems

The magnetic form factors of $^2$H, $^3$H, and $^3$He, deuteron photodisintegration cross sections at low energies, and deuteron threshold electrodisintegration cross sections at backward angles in a wide range of momentum transfers, are calculated with the chiral two-nucleon (and three-nucleon) interactions including $\Delta$ intermediate states that have recently been constructed in configuration space. The $A\,$=$\,$3 wave functions are obtained from hyperspherical-harmonics solutions of the Schr\"odinger equation. The electromagnetic current includes one- and two-body terms, the latter induced by one- and two-pion exchange (OPE and TPE, respectively) mechanisms and contact interactions. The contributions associated with $\Delta$ intermediate states are only retained at the OPE level, and are neglected in TPE loop (tree-level) corrections to two-body (three-body) current operators. Expressions for these currents are derived and regularized in configuration space for consistency with the interactions. The low-energy constants that enter the contact few-nucleon systems. The predicted form factors and deuteron electrodisintegration cross section are in excellent agreement with experiment for momentum transfers up to 2--3 fm$^{-1}$. However, the experimental values for the deuteron photodisintegration cross section are consistently underestimated by theory, unless use is made of the Siegert form of the electric dipole transition operator. A complete analysis of the results is provided, including the clarification of the origin of the aforementioned discrepancy.Comment: 24 pages, 13 figure

Ocorrência de Anastrepha zenildae Zucchi (Diptera: Tephritidae) e seu parasitóide em frutos de Ziziphus mauritiana (Rhamnaceae) em Roraima.

Resumo 1626-1

Extensive solitary lymphatic malformation of the liver in a child: a case report and literature review

Intrabdominal lymphatic malformations are rare benign congenital vascular anomalies that account for less than 5% of benign masses in childhood, with an extremely variable clinical presentation. For this reason, although their radiological appearance is usually typical, diagnosis can be challenging and not always immediate. This report describes a unique case of extensive solitary hepatic lymphatic malformation in a 10-year-old boy with both extra- and intraparenchymal development with no associated symptoms. A literature review of reported cases of solitary hepatic lymphatic malformation is also included

Construction of a synthetic infectious cDNA clone of Grapevine Algerian latent virus (GALV-Nf) and its biological activity in Nicotiana benthamiana and grapevine plants

Background: Grapevine Algerian latent virus (GALV) is a tombusvirus first isolated in 1989 from an Algerian grapevine (Vitis spp.) plant and more recently from water samples and commercial nipplefruit and statice plants. No further reports of natural GALV infections in grapevine have been published in the last two decades, and artificial inoculations of grapevine plants have not been reported. We developed and tested a synthetic GALV construct for the inoculation of Nicotiana benthamiana plants and different grapevine genotypes to investigate the ability of this virus to infect and spread systemically in different hosts. Methods: We carried out a phylogenetic analysis of all known GALV sequences and an epidemiological survey of grapevine samples to detect the virus. A GALV-Nf clone under the control of the T7 promoter was chemically synthesized based on the full-length sequence of the nipplefruit isolate GALV-Nf, the only available sequence at the time the project was conceived, and the infectious transcripts were tested in N. benthamiana plants. A GALV-Nf-based binary vector was then developed for the agroinoculation of N. benthamiana and grapevine plants. Infections were confirmed by serological and molecular analysis and the resulting ultrastructural changes were investigated in both species. Results: Sequence analysis showed that the GALV coat protein is highly conserved among diverse isolates. The first epidemiological survey of cDNAs collected from 152 grapevine plants with virus-like symptoms did not reveal the presence of GALV in any of the samples. The agroinoculation of N. benthamiana and grapevine plants with the GALV-Nf binary vector promoted efficient infections, as revealed by serological and molecular analysis. The GALV-Nf infection of grapevine plants was characterized in more detail by inoculating different cultivars, revealing distinct patterns of symptom development. Ultrastructural changes induced by GALV-Nf in N. benthamiana were similar to those induced by tombusviruses in other hosts, but the cytopathological alterations in grapevine plants were less severe. Conclusions: This is the first report describing the development of a synthetic GALV-Nf cDNA clone, its artificial transmission to grapevine plants and the resulting symptoms and cytopathological alterations

Levantamento de hospedeiros e parasitóides de Anastrepha spp. (Diptera: Tephritidae) no município de Boa Vista, Estado de Roraima.

Resumo 937-1

Efeito da tostagem de barricas de carvalho sobre o perfil químico e sensorial de vinhos tintos.

XV Congresso Latino-Americano de Viticultura e Enologia E XIII Congresso Brasileiro de Viticultura e Enologia. Bento Gonçalves-RS, 3 a 7 de Novembro de 2015

Bimodal release ondansetron for acute gastroenteritis among adolescents and adults: A randomized clinical trial

Importance: Vomiting resulting from acute gastroenteritis is commonly treated with intravenous antiemetics in acute care settings. If oral treatment were beneficial, patients might not need intravenous administered hydration or medication. Furthermore, a long-acting treatment could provide sustained relief from nausea and vomiting. Objective: To determine whether an experimental long-acting bimodal release ondansetron tablet decreases gastroenteritis-related vomiting and eliminates the need for intravenous therapy for 24 hours after administration. Design, Setting, and Participants: This placebo-controlled, double-blind, randomized clinical trial included patients from 19 emergency departments and 2 urgent care centers in the United States from December 8, 2014, to February 17, 2017. Patients 12 years and older with at least 2 vomiting episodes from presumed gastroenteritis in the previous 4 hours and symptoms with less than 36 hours\u27 duration were randomized using a 3:2 active to placebo ratio. Analyses were performed on an intent-to-treat basis and conducted from June 1, 2017, to November 1, 2017. Intervention: Bimodal release ondansetron tablet containing 6 mg of immediate release ondansetron and 18 mg of a 24-hour release matrix for a total of 24 mg of ondansetron. Main Outcomes and Measures: Treatment success was defined as no further vomiting, no need for rescue medication, and no intravenous hydration for 24 hours after bimodal release ondansetron administration. Results: Analysis included 321 patients (mean [SD] age, 29.0 [11.1] years; 195 [60.7%] women), with 192 patients in the bimodal release ondansetron group and 129 patients in the placebo group. Treatment successes were observed in 126 patients in the bimodal release ondansetron group (65.6%) compared with 70 patients in the placebo group (54.3%), with an 11.4% (95% CI, 0.3%-22.4%) absolute probability difference. The proportion of treatment success was 21% higher among patients who received bimodal release ondansetron compared with those who received a placebo (relative risk, 1.21; 95% CI, 1.00-1.46; P = .04). In an analysis including only patients with a discharge diagnosis of acute gastroenteritis and no major protocol violations, there were 123 treatment successes (69.5%) in the bimodal release ondansetron group compared with 67 treatment successes (54.9%) in the placebo group (relative risk, 1.27; 95% CI, 1.05-1.53; P = .01). Adverse effects were infrequent and similar to the known safety profile of ondansetron. Conclusions and Relevance: This randomized clinical trial found that a long-acting bimodal release oral ondansetron tablet was an effective antiemetic among adolescents and adults with moderate to severe vomiting from acute gastroenteritis. The drug benefits extended to 24 hours after administration. Bimodal release ondansetron may decrease the need for intravenous access and emergency department care to manage acute gastroenteritis. Trial Registration: ClinicalTrials.gov identifier: NCT02246439

Transcatheter Mitral Valve-in-Valve Dislocation: A Rescue Strategy

In the transcatheter aortic valve replacement era, transapical valve-in-valve mitral valve implantation is considered an alternative treatment for high-risk patients with degenerated mitral bioprosthesis. We report an unusual strategy to remedy the potentially fatal complication of the mitral prosthesis migration into the aortic arch. The dislocated prosthesis was successfully stabilized in the aortic arch with a bare aortic stent, ensuring adequate perfusion of epiaortic vessels. \ua9 2018 The Society of Thoracic Surgeon

Local chiral interactions, the tritium Gamow-Teller matrix element, and the three-nucleon contact term

The Gamow-Teller (GT) matrix element contributing to tritium $\beta$ decay is calculated with trinucleon wave functions obtained from hyperspherical-harmonics solutions of the Schr\"odinger equation with the chiral two- and three-nucleon interactions including $\Delta$ intermediate states that have recently been constructed in configuration space. Predictions up to N3LO in the chiral expansion of the axial current (with $\Delta$'s) overestimate the empirical value by 1--4 \%. By exploiting the relation between the low-energy constant (LEC) in the contact three-nucleon interaction and two-body axial current, we provide new determinations of the LECs $c_D$ and $c_E$ that characterize this interaction by fitting the trinucleon binding energy and tritium GT matrix element. Some of the implications that the resulting models of three-nucleon interactions have on the spectra of light nuclei and the equation of state of neutron matter are briefly discussed. We also provide a partial analysis, which ignores $\Delta$'s, of the contributions due to loop corrections in the axial current at N4LO. Finally, explicit expressions for the axial current up to N4LO have been derived in configuration space, which other researchers in the field may find useful.Comment: 16 pages, 3 figure