Cytological Studies On Banisteriopsis C.b. Robinson Ex Small And Heteropterys Kunth (malpighiaceae)

Cytological studies were carried out on 6 species of Banisteriopsis and 4 species of Heteropterys (Malpighiaceae, Malpighioideae), collected in forest and cerrado areas of southeastern Brazil, and a cultivated one. No previous chromosome counts are known for 3 species of Banisteriopsis and 3 species of Heteroprerys. Chromosome numbers were obtained through mitotic (7 counts) and meiotic (5 counts) analysis. No meiotic abnormalities were encountered in the species studied, which presented high viable pollen rates. For the first time, karyomorphological characters were found for both genera, with a predominance of metacentric chromosomes. The chromosome number variation, based on x=5 and 10, with cases of polyploidy, and the similarity between the 2 related genera are discussed.663253259Anderson, W.R., Byrsonimoideae, a new sub-family of Malpighiaceae (1977) Biotropica, 7, pp. 5-18Floral conservatism in neotropical Malpighiaceae (1979) Biotropica, 11, pp. 219-223Chromosome numbers of neotropical Malpighiaceae (1993) Contr. Univ. Mich. Herb., 17, pp. 21-37Baldwin, J.T., Banisteria caapi Spruce: It's chromosomes (1946) Bull. Torrey Bot. Club., 73, pp. 282-285Bawa, K.S., Chromosome number of tree species of lowland tropical community (1973) J. Arnold. Arbor., 54, pp. 422-434Chen, Z.Y., Huang, S.F., The karyotype of five cultivated plants (1989) Acta Bot. Austro Sin., 4, pp. 75-83DiFulvio, T.E., Número cromossómico de Heteropteris hypvercifolia (Malpighiaceae) (1979) Kurtziana, 12-13, p. 139Forni-Martins, E.R., Pinto-Maglio, C.A., Da Cruz, N.D., IOPB chromosome data I (1989) Int. Organ. Pl. Biosyst. Newslett. (Zurich), 13, p. 17Biologia da reprodução de plantas de cerrado: Microsporogênese (1992) Ann. VIII Congr. Soc. Bot. S.P., pp. 77-82Chromosome numbers in Brazilian cerrado plants (1995) Rev. Bras. Genét., 18, pp. 281-288Fouët, M., Contribution à l'étude cyto-taxonomique des Malpihghiacées (1966) Adansonia, 6, pp. 456-505Gates, B., Banisteriopsis, Diplopterys (Malpighiaceae) (1982) Flora Neotropica Monograph, 30, pp. 1-236Guerra, M.S., O uso do corante Giemsa na citogenética vegetal-comparação simples e o bandeamento (1983) Ciênc. Cult., 35, pp. 190-193Reviewing the chromosome nomenclature of Levan et al., short comunication (1986) Rev. Bras. Genét., 9, pp. 741-743Guanabara Koogan, S.A., (1986) Introdução à Citogenética Geral, p. 142. , Rio de Janeiro, R.JHuziwara, Y., Karyotype analysis in some genera of Compositae. VIII. Further studies on the chromosome of Aster (1962) Am. J. Bot., 49, pp. 116-119Lombello, R.A., Forni-Martins, E.R., Cytological studies in climbers of a Brazilian Forest Reserve (1998) Cytologia, 63, pp. 415-420Maffei, E.M.D., Marin-Morales, M.A., Ruas, P.M., Ruas, C.F., Matzenbacher, N.I., Chromosomal polymorphism in 12 populations of Mikania micrantha (Compositae) (1999) Gen. Mol. Biol., 22, pp. 433-444Makino-Watanabe, H., Melhem, T.S., Barth, O.M., Pollen morphology of Banisteriopsis C. B. Robinson ex Small (Malpighiaceae) (1993) Rev. Bras. Bot., 16, pp. 47-68Mangenot, S., Mangenot, G., Enquête sur les nombres chromosomiques dans une collection d'espèces tropicales (1962) Rev. Cyt. Biol. Vég., 25, pp. 411-447Medina, D.M., Conagin, C.H.T.M., (1964) Técnica citológica., , Publicação 2610. Campinas, Inst. AgronMorawetz, W., Karyological races and ecology of the Brazilian Duguetia furfuracea as compared with Xylopia aromatica (Annonaceae) (1984) Flora, 175, pp. 195-209Niedenzu, F., Malpighiaceae (1928) Das Pflanzenreich, , Engler, A. (ed.). Im Auftrage der Preuss, Akademie der WissenchaftenOrmond, W.T., Silva, M.I.A., Castells, A.R.L., Contribuição ao estudo citológico de Malpighiaceae. I. Número de cromossomos (1981) Arch. Jard. Bot. R. J., 25, pp. 169-173Pal, M., Chromosome numbers in some Indian angiosperms. I (1964) Proc. Indian Acad. Sci., 60, pp. 347-350Roy, R.P., Mishra, N.C., Cytological studies in Malpighiaceae (1962) Proc. 49th Indian Sci. Congr., 3, p. 335Semple, J.C., Chromosome number of phanerogam 4 (1970) Ann. Miss. Bot. Gard., 57, pp. 382-384Singhal, V.K., Gill, B.S., Bir, S.S., Cytological studies in some members of Malpighiaceae (1985) Cytologia, 50, pp. 1-8Stebbins G.L., Jr., Chromosomal variation in higher plants (1971), p. 216. , Edward Arnold Ltd., LondonVogel, S., History of the Malpighiaceae in the light of pollination ecology (1990) Mem. New York Bot. Gard., 55, pp. 130-14

Cytogenetics And Evolutionary Analysis Of Lophanthera, An Amazonian Arboreal Malpighiaceae

Only 2 chromosome counts are reported for Lophanthera, an Amazonian arboreal genus with 5 species that belongs to sub-family Byrsonimoideae. Cytological studies were carried out in Lophanthera lactescens Ducke to verify the haploid and diploid chromosome numbers (n=6, 2n=12) of a previously unstudied population. The meiotic process was regular and no abnormalities were found. The first chromosomal ideogram for the genus was constructed and shows a predominance of metacentric pairs (5 m + 1 sm). In order to explain the increased lengths of its chromosomes (5.3 μm to 11.8 μm) and the primitive position of the genus in Malpighiaceae, in situ hybridization with a telomeric probe was performed. We observed the distribution of the telomeric sequences. No interstitial sequences were found. In addition to the symmetric karyotype observed, this assay indicates that probably no fusion or translocations of segments has occurred along the karyotype evolution of Lophanthera.6714145Adams, S.P., Leitch, I.J., Bennett, M.D., Leitch, A.R., Aloe L., a second plant family without (TTTAGGG)n telomeres (2000) Chromosoma, 109, pp. 201-205Anderson, W.R., Brysonimoideae, a new sub-family of the Malpighiaceae (1977) Leandra, 7, pp. 5-18Malpighiaceae Mem. New York Bot. Gard., 32, pp. 21-305. , MAGUIRE, B. and Col. The Botany of the Guyana Highland--XILophanthera, a genus of Malpighiaceae new to Central America (1983) Brittonia, 35, pp. 37-41Devar, K.V., Boraiah, G., A note on the karyomorphology of Hiptage benghalensis (L.) Kurz (1981) Curr. Sci., 50, pp. 904-905Fuchs, J., Brandes, A., Schubert, I., Telomere sequence localization and karyotype evolution in higher plants (1995) Plant Syst. Evol., 196, pp. 227-241Ganal, M.W., Lapitan, N.L.V., Tanksley, S.D., Macrostructure of the tomato telomeres (1991) Plant Cell, 3, pp. 87-94Guerra, M.S., Reviewing the chromosome nomenclature of Levan et al. Short communication (1986) Rev. Bras. Genét., 9, pp. 741-743Kenton, A., Distribution of telomere DNA in mitotic and polytene nuclei of author tapetum of a tetraploid hybrid bean, Phaseolus vulgaris ×P. acuifolius (1996) Braz. J. Gen., 19, pp. 313-318Huziwara, Y., Karyotype analysis in some genera of Compositeae VIII. Further studies on the chromosomes of Aster (1962) Am. J. Botany, 49, pp. 116-119Leitch, I.J., Heslop-Harrison, J.S., Physical mapping of the 8S-26S rDNA genes in barley by in situ hybridization (1992) Genome, 35, pp. 1013-1018Lewis, W.H., Oliver, R.L., (1970) Ann. Missouri Bot. Gard., 57, pp. 382-384. , Chromosome Numbers of Phanerogams 4Lombello, R.A., (2000) Estudos cromossômicos em Malpighiaceae A. Jussieu, , PhD thesis, Department of Botany, State University of CampinasForni-Martins, E.R., Cytological studies in climbers of a Brazilian Forest Reserve (1998) Cytologia, 63, pp. 415-420Kilian, A., Carol, S., Kleinhofs, A., Barley telomeres shorten during differentiation but grow in callus culture (1995) Proc. Natl. Acad. Sci. USA, 92, pp. 9555-9559Pal, M., Chromosome numbers in some angiosperms I (1964) Proc. Indian Acad. Sci., 60, pp. 347-350Raven, P.H., The bases of Angiosperm phylogeny: Cytology (1975) Ann. Missouri Bot. Gard., 62, pp. 724-764Richards, E.J., Ausubel, F.M., Isolation of a higher eukaryotic telomere from Arabidopsis thaliana (1988) Cell, 53, pp. 127-136Schwarzacher, T., Heslop-Harrison, J.S., In situ hibridization to plant telomeres using synthetic oligimers (1991) Genome, 34, pp. 317-323Seavey, S., (1975) Ann. Missouri Bot. Gard., 57, pp. 382-384. , Chromosome Numbers of Phanerogams 4Slijepcevic, P., Telomeres and mechanisms of Robertsonian fusion (1998) Chromosoma, 107, pp. 136-140Stebbins Jr., G.L., (1971) Chromosomal Variation in Higher Plants, , Edward Arnold Publish, LondonViegas-Péquinot, E., In situ hybridization to chromosomes with biotinylated probes (1992) In situ Hybridization: A Practical Approach, pp. 137-158. , Willerson, D. (ed.). Oxford University Press, IRL PressWang, S., Lapitain, N.L.V., Characterization of telomeres in Hordeum vulgare chromosomes by in situ hybridization. II. Healed broken chromosomes in telotrisomic 4L and acrotrisomic 4L4S lines (1992) Genome, 35, pp. 975-980Watson, L., Dallwitz, M.J., (1992) The families of flowering plants: descriptions, illustrations, identification and information retrieval, , Http://biodiversity.uno.edu/delta, Version: 27th September 2000Zaman, M.A., Patwary, M.U., Matin, A., Karyomorphology and meiotic behavior of Galphimia gracilis Bartl. (Malpighiaceae) (1977) Caryologia, 30, pp. 429-43

Natural infection of several Coffea species and hybrids and Psilanthus ebracteolatus by the coffee ringspot virus (CoRSV)

Coffee ringspot is a minor coffee disease caused by the nuclear type of Brevipalpus mite-transmitted virus, Coffee ringspot virus (CoRSV). Recently outbreaks of the disease in some growing regions of the state of Minas Gerais, Brazil, were registered with qualitative and quantitative yield losses. Coffea arabica was the only species registered as natural host. A survey was made on a germplasm collection of Coffea and related species kept at the Centro de Café "Alcides Carvalho", Instituto Agronômico, Campinas, state of São Paulo (SP), Brazil, to assess natural susceptibility of Coffee species, other than C. arabica and some interspecific hybrids of Coffea as well as other non-Coffea plant species to the Coffee ringspot virus (CoRSV). The following plants were found with ringspot symptoms on their leaves and/or fruits besides C. arabica L.: C. kapakata (IAC 4511), C. dewevrei cv. Excelsa, C. canephora cv. Robusta, hybrid derivative of the C. arabica × C. racemosa (IAC1195-5-6-2), C. arabica × C. dewerei (Piatã IAC 387), Híbrido de Timor CIFC 832/1 (derivative from a natural crossing between C. arabica × C. canephora) and C. racemosa. Also Psilanthus ebracteolatus, a species close to the genus Coffee was also found with ringspot lesions on their leaves. All these plants were also found infested by Brevipalpus mites identified as B. phoenicis. Infection of these plants by CoRSV was confirmed by the observation of characteristic cytopathic effects in the tissues of the lesion and by RT-PCR using a pair of primer specific for CoRSV. Only with C. racemosa RT-PCR failed to amplify the CoRSV genome. The susceptibility of P. ebracteolatus to CoRSV adds new dimension regarding its controversial taxonomic position