The role of strain hardening in the transition from dislocation-mediated to frictional deformation of marbles within the Karakoram Fault Zone, NW India

The onset of frictional failure and potentially seismogenic deformation in carbonate rocks undergoing exhumation within fault zones depends on hardening processes that reduce the efficiency of aseismic dislocation-mediated deformation as temperature decreases. However, few techniques are available for quantitative analysis of dislocation slip system activity and hardening in natural tectonites. Electron backscatter diffraction maps of crystal orientations offer one such approach via determination of Schmid factors, if the palaeostress conditions can be inferred and the critical resolved shear stresses of slip systems are constrained. We analyse calcite marbles deformed in simple shear within the Karakoram Fault Zone, NW India, to quantify changes in slip system activity as the rocks cooled during exhumation. Microstructural evidence demonstrates that between ∼300 °C and 200–250 °C the dominant deformation mechanisms transitioned from dislocation-mediated flow to twinning and frictional failure. However, Schmid factor analysis, considering critical resolved shear stresses for yield of undeformed single crystals, indicates that the fraction of grains with sufficient resolved shear stress for glide apparently increased with decreasing temperature. Misorientation analysis and previous experimental data indicate that strain-dependent work hardening is responsible for this apparent inconsistency and promoted the transition from dislocation-mediated flow to frictional, and potentially seismogenic, deformation.David Wallis and Lars Hansen acknowledge support from the Natural Environment Research Council grant NE/M000966/1

High-Angular Resolution Electron Backscatter Diffraction as a New Tool for Mapping Lattice Distortion in Geological Minerals

Analysis of distortions of the crystal lattice within individual mineral grains is central to the investigation of microscale processes that control and record tectonic events. These distortions are generally combinations of lattice rotations and elastic strains, but a lack of suitable observational techniques has prevented these components being mapped simultaneously and routinely in earth science laboratories. However, the technique of high-angular resolution electron backscatter diffraction (HR-EBSD) provides the opportunity to simultaneously map lattice rotation and elastic strain gradients with exceptional precision, on the order of 0.01° for rotations and 10−4 in strain, using a scanning electron microscope. Importantly, these rotations and lattice strains relate to densities of geometrically necessary dislocations and residual stresses. Recent works have begun to apply and adapt HR-EBSD to geological minerals, highlighting the potential of the technique to provide new insights into the microphysics of rock deformation. Therefore, the purpose of this review is to provide a summary of the technique, to identify caveats and targets for further development, and to suggest areas where it offers potential for major advances. In particular, HR-EBSD is well suited to characterizing the roles of different dislocation types during crystal plastic deformation and to mapping heterogeneous internal stress fields associated with specific deformation mechanisms/microstructures or changes in temperature, confining pressure, or macroscopic deviatoric stress. These capabilities make HR-EBSD a particularly powerful new technique for analyzing the microstructures of deformed geological materials.D. Wallis, L. N. Hansen, and A. J. Wilkinson acknowledge support from the Natural Environment Research Council grant NE/M0009661. T. B. Britton acknowledges support for his research fellowship from the Royal Academy of Engineering

Oriented grain growth and modification of ‘frozen anisotropy’ in the lithospheric mantle

Seismic anisotropy throughout the oceanic lithosphere is often assumed to be generated by fossilized texture formed during deformation at asthenospheric temperatures close to the ridge. Here we investigate the effect of high-temperature and high-pressure static annealing on the texture of previously deformed olivine aggregates to simulate residence of deformed peridotite in the lithosphere. Our experiments indicate that the orientation and magnitude of crystallographic preferred orientation (CPO) will evolve due to the preferential growth of grains with low dislocation densities. These observations suggest that texture and stored elastic strain energy promote a style of grain growth that modifies the CPO of a deformed aggregate. We demonstrate that these microstructural changes alter the orientation distributions and magnitudes of seismic wave velocities and anisotropy. Therefore, static annealing may complicate the inference of past deformation kinematics from seismic anisotropy in the lithosphere.This research is supported by NSF EAR-1131985 (to PS), with additional support from the Institute of Materials Science and Engineering at Washington University in St. Louis. DW and LNH acknowledge support from the Natural Environment Research Council Grant NE/M000966/1

Transient Creep in Subduction Zones by Long-Range Dislocation Interactions in Olivine

Large earthquakes transfer stress from the shallow lithosphere to the underlying viscoelastic lower crust and upper mantle, inducing transient creep during the postseismic interval. Recent experiments on olivine have provided a new rheological model for this transient creep based on accumulation and release of back stresses among dislocations. Here, we test whether natural rocks preserve dislocation-induced stress heterogeneity consistent with the back-stress hypothesis by mapping olivine from the palaeosubduction interface of the Oman-UAE ophiolite with high-angular resolution electron backscatter diffraction. The olivine preserves heterogeneous residual stresses that vary in magnitude by several hundred megapascals over length scales of a few micrometres. Large stresses are commonly spatially associated with elevated densities of geometrically necessary dislocations within subgrain interiors. These spatial relationships, along with characteristic probability distributions of the stresses, confirm that the stress heterogeneity is generated by the dislocations and records their long-range elastic interactions. Images of dislocations decorated by oxidation display bands of high and low dislocation density, suggesting that dislocation interactions contributed to organisation of the substructure. These results support the applicability of the back-stress model of transient creep to deformation in the mantle portion of plate-boundary shear zones. The model predicts that rapid stress changes, such as those imposed by large earthquakes, can induce order-of-magnitude changes in viscosity that depend nonlinearly on the stress change, consistent with inferences of mantle rheology from geodetic observations.This work was supported by the Natural Environment Research Council, grant NE/M000966/1; the Netherlands Organisation for Scientific Research, User Support Programme Space Research, grant ALWGO.2018.038; and startup funds from Utrecht University

Low-Frequency Measurements of Seismic Moduli and Attenuation in Antigorite Serpentinite

Laboratory measurements of seismic moduli and attenuation in antigorite serpentinite at a confining pressure of 200 MPa and temperatures up to 550 °C provide new results relevant to the interpretation of geophysical data in subduction zones. A polycrystalline antigorite specimen was tested via forced oscillations at small strain amplitudes and seismic frequencies (millihertz to hertz). The shear modulus has a temperature sensitivity, ∂G/∂T, averaging −0.017 GPa/K. Increasing temperature above 500 °C results in more intensive shear attenuation ( equation/grl58579-math-0001.png) and associated modulus dispersion, with equation/grl58579-math-0002.png increasing monotonically with increasing oscillation period and temperature. This “background” relaxation is adequately captured by a Burgers model for viscoelasticity and possibly results from intergranular mechanisms. Attenuation is higher in antigorite ( equation/grl58579-math-0003.png at 550 °C and 0.01 Hz) than in olivine ( equation/grl58579-math-0004.png below 800 °C), but such contrast does not appear to be strong enough to allow robust identification of antigorite from seismic models of attenuation only

Tutorial: Crystal orientations and EBSD - Or which way is up?

Electron backscatter diffraction (EBSD) is an automated technique that can measure the orientation of crystals in a sample very rapidly. There are many sophisticated software packages that present measured data. Unfortunately, due to crystal symmetry and differences in the set-up of microscope and EBSD software, there may be accuracy issues when linking the crystal orientation to a particular microstructural feature. In this paper we outline a series of conventions used to describe crystal orientations and coordinate systems. These conventions have been used to successfully demonstrate that a consistent frame of reference is used in the sample, unit cell, pole figure and diffraction pattern frames of reference. We establish a coordinate system rooted in measurement of the diffraction pattern and subsequently link this to all other coordinate systems. A fundamental outcome of this analysis is to note that the beamshift coordinate system needs to be precisely defined for consistent 3D microstructure analysis. This is supported through a series of case studies examining particular features of the microscope settings and/or unambiguous crystallographic features. These case studies can be generated easily in most laboratories and represent an opportunity to demonstrate confidence in use of recorded orientation data. Finally, we include a simple software tool, written in both MATLAB® and Python, which the reader can use to compare consistency with their own microscope set-up and which may act as a springboard for further offline analysis.The authors would like to thank a range of funders that underpin this collaborative work: T.B. Britton has a fellowship from the Royal Academy of Engineering. J. Jiang is funded by AVIC BIAM. T.B. Britton and A.J. Wilkinson have project funding from EPSRC through the HexMat programme grant (www.imperial.ac.uk/hexmat EP/K034332/1). DW, A.J. Wilkinson and L. Hanson have project funding from NERC through NE/M000966/1. A.J. Wilkinson and A. Vilalta-Clemente have project funding from EPSRC through EP/J016098/1

High-angular resolution electron backscatter diffraction as a new tool for mapping lattice distortion in geological minerals

Analysis of distortions of the crystal lattice within individual mineral grains is central to the investigation of microscale processes that control and record tectonic events. These distortions are generally combinations of lattice rotations and elastic strains, but a lack of suitable observational techniques has prevented these components being mapped simultaneously and routinely in earth science laboratories. However, the technique of high‐angular resolution electron backscatter diffraction (HR‐EBSD) provides the opportunity to simultaneously map lattice rotation and elastic strain gradients with exceptional precision, on the order of 0.01° for rotations and 10−4 in strain, using a scanning electron microscope. Importantly, these rotations and lattice strains relate to densities of geometrically necessary dislocations and residual stresses. Recent works have begun to apply and adapt HR‐EBSD to geological minerals, highlighting the potential of the technique to provide new insights into the microphysics of rock deformation. Therefore, the purpose of this review is to provide a summary of the technique, to identify caveats and targets for further development, and to suggest areas where it offers potential for major advances. In particular, HR‐EBSD is well suited to characterizing the roles of different dislocation types during crystal plastic deformation and to mapping heterogeneous internal stress fields associated with specific deformation mechanisms/microstructures or changes in temperature, confining pressure, or macroscopic deviatoric stress. These capabilities make HR‐EBSD a particularly powerful new technique for analyzing the microstructures of deformed geological materials

An alginate-layer technique for culture of Brassica oleracea L. protoplasts

Ten accessions belonging to the Brassica oleracea subspecies alba and rubra, and to B. oleracea var. sabauda were used in this study. Protoplasts were isolated from leaves and hypocotyls of in vitro grown plants. The influence of selected factors on the yield, viability, and mitotic activity of protoplasts immobilized in calcium alginate layers was investigated. The efficiency of protoplast isolation from hypocotyls was lower (0.7 ± 0.1 × 106 ml−1) than for protoplasts isolated from leaf mesophyll tissue (2 ± 0.1 × 106 ml−1). High (70–90%) viabilities of immobilized protoplasts were recorded, independent of the explant sources. The highest proportion of protoplasts undergoing divisions was noted for cv. Reball F1, both from mesophyll (29.8 ± 2.2%) and hypocotyl (17.5 ± 0.3%) tissues. Developed colonies of callus tissue were subjected to regeneration and as a result plants from six accessions were obtained

Crystallographic Preferred Orientation of Olivine in Sheared Partially Molten Rocks: The Source of the “a-c Switch”

© 2018. American Geophysical Union. All Rights Reserved. To investigate the mechanism that produces the crystallographic preferred orientations (CPO) characteristic of sheared partially molten rocks of mantle composition, we analyzed the microstructures of samples of olivine plus 7% basaltic melt deformed in torsion to shear strains as large as γ = 13.3 Electron backscattered diffraction (EBSD) observations reveal a CPO characterized by a weak a-c girdle in the shear plane that develops by γ = 4. This CPO, which exhibits a slightly stronger alignment of 001 than 100 axes in the shear direction, changes little in both strength and distribution with increasing stress and with increasing strain. Furthermore, it is significantly weaker than the CPO observed for dry, melt-free olivine aggregates. Orientation maps correlated with grain shape measurements from tangential, radial, and transverse sections indicate that olivine grains are longer along 001 axes than along 100 axes and shortest along 010 axes. This morphology is similar to that of olivine grains in a mafic melt. We conclude that the weak a-c girdle observed in sheared partially molten rocks reflects contributions from two processes. Due to their shape-preferred orientation (SPO), grains rotate to align their 001 axes parallel to the flow direction. At the same time, dislocation glide on the (010)100 slip system rotates 100 axes into the flow direction. The presence of this CPO in partially molten regions of the upper mantle significantly impacts the interpretation of seismic anisotropy and kinematics of flow

Risk of hypoglycaemia with insulin degludec versus insulin glargine U300 in insulin-treated patients with type 2 diabetes : the randomised, head-to-head CONCLUDE trial

Aims/hypothesis A head-to-head randomised trial was conducted to evaluate hypoglycaemia safety with insulin degludec 200 U/ml (degludec U200) and insulin glargine 300 U/ml (glargine U300) in individuals with type 2 diabetes treated with basal insulin. Methods This randomised (1:1), open-label, treat-to-target, multinational trial included individuals with type 2 diabetes, aged ≥18 years with HbA1c ≤80 mmol/mol (9.5%) and BMI ≤45 kg/m2. Participants were previously treated with basal insulin with or without oral glucose-lowering drugs (excluding insulin secretagogues) and had to fulfil at least one predefined criterion for hypoglycaemia risk. Both degludec U200 and glargine U300 were similarly titrated to a fasting blood glucose target of 4.0–5.0 mmol/l. Endpoints were assessed during a 36 week maintenance period and a total treatment period up to 88 weeks. There were three hypoglycaemia endpoints: (1) overall symptomatic hypoglycaemia (either severe, an event requiring third-party assistance, or confirmed by blood glucose [<3.1 mmol/l] with symptoms); (2) nocturnal symptomatic hypoglycaemia (severe or confirmed by blood glucose with symptoms, between 00:01 and 05:59 h); and (3) severe hypoglycaemia. The primary endpoint was the number of overall symptomatic hypoglycaemic events in the maintenance period. Secondary hypoglycaemia endpoints included the number of nocturnal symptomatic events and number of severe hypoglycaemic events during the maintenance period. Results Of the 1609 randomised participants, 733 of 805 (91.1%) in the degludec U200 arm and 734 of 804 (91.3%) in the glargine U300 arm completed the trial (87.3% and 87.8% completed on treatment, respectively). Baseline characteristics were comparable between the two treatment arms. For the primary endpoint, the rate of overall symptomatic hypoglycaemia was not significantly lower with degludec U200 vs glargine U300 (rate ratio [RR] 0.88 [95% CI 0.73, 1.06]). As there was no significant difference between treatments for the primary endpoint, the confirmatory testing procedure for superiority was stopped. The pre-specified confirmatory secondary hypoglycaemia endpoints were analysed using pre-specified statistical models but were now considered exploratory. These endpoints showed a lower rate of nocturnal symptomatic hypoglycaemia (RR 0.63 [95% CI 0.48, 0.84]) and severe hypoglycaemia (RR 0.20 [95% CI 0.07, 0.57]) with degludec U200 vs glargine U300. Conclusions/interpretation There was no significant difference in the rate of overall symptomatic hypoglycaemia with degludec U200 vs glargine U300 in the maintenance period. The rates of nocturnal symptomatic and severe hypoglycaemia were nominally significantly lower with degludec U200 during the maintenance period compared with glargine U300