1,424 research outputs found
Unconventional Cosmology
I review two cosmological paradigms which are alternative to the current
inflationary scenario. The first alternative is the "matter bounce", a
non-singular bouncing cosmology with a matter-dominated phase of contraction.
The second is an "emergent" scenario, which can be implemented in the context
of "string gas cosmology". I will compare these scenarios with the inflationary
one and demonstrate that all three lead to an approximately scale-invariant
spectrum of cosmological perturbations.Comment: 45 pages, 10 figures; invited lectures at the 6th Aegean Summer
School "Quantum Gravity and Quantum Cosmology", Chora, Naxos, Greece, Sept.
12 - 17 2012, to be publ. in the proceedings; these lecture notes form an
updated version of arXiv:1003.1745 and arXiv:1103.227
Magnetic resonance imaging of glutamate in neuroinflammation
AbstractInflammation in central nervous system (CNS) is one of the most severe diseases, and also plays an impellent role in some neurodegenerative diseases. Glutamate (Glu) has been considered relevant to the pathogenesis of neuroinflammation. In order to diagnose neuroinflammation incipiently and precisely, we review the pathobiological events in the early stages of neuroinflammation, the interactions between Glu and neuroinflammation, and two kinds of magnetic resonance techniques of imaging Glu (chemical exchange saturation transfer and magnetic resonance spectroscopy)
Syncytialization and prolonged exposure to palmitate impacts bewo respiration
Placental villous trophoblast mitochondrial respiratory function is critical for a successful pregnancy and environmental influences such as maternal obesity have been associated with respiratory impairment at term. More recently, a gestational high fat diet independent of maternal body composition, has been highlighted as a potential independent regulator of placental mitochondrial metabolism. The current study aimed to characterize the direct impact of a prolonged and isolated exposure to the dietary fatty acids Palmitate (PA) and Oleate (OA) upon placental cell mitochondrial respiratory function. BeWo cytotrophoblast (CT) and syncytiotrophoblast (SCT) cells were treated for 72 h with 100 μM PA, OA or PA+OA (P/O). Live-cell metabolic function was analyzed via the Seahorse XF Mito and Glycolysis Stress tests. Immunoblots and spectrophotometric activity assays were utilized to examine the protein expression and function of electron transport chain (ETC) complexes and key mitochondrial regulatory enzymes. Syncytialization of BeWo cells resulted reduced respiratory activity in conjunction with altered complex I and II activity and decreased pyruvate dehydrogenase (PDH) protein expression and activity. PA and P/O treatments were associated with increased basal and maximal respiratory activities in BeWo CT cells without alterations in protein expression or activity of individual ETC complexes and mitochondrial substrate regulators. The metabolic suppression in BeWo SCTs was consistent with that previously observed in primary human trophoblast cell cultures, while the observed increases in respiratory activity in PA-treated BeWo CTs may be indicative of an early timepoint of specific dietary saturated fat-mediated placental cell mitochondrial dysfunction
Peroxisome proliferator-activated receptor -β/δ, -γ Agonists and resveratrol modulate hypoxia induced changes in nuclear receptor activators of muscle oxidative metabolism
PPAR-α, PPAR-β, and PPAR-γ, and RXR in conjunction with PGC-1α and SIRT1, activate oxidative metabolism genes determining insulin sensitivity. In utero, hypoxia is commonly observed in Intrauterine Growth Restriction (IUGR), and reduced insulin sensitivity is often observed in these infants as adults. We sought to investigate how changes in oxygen tension might directly impact muscle PPAR regulation of oxidative genes. Following eight days in culture at 1 oxygen, C2C12 muscle myoblasts displayed a reduction of PGC-1α, PPAR-α, and RXR-α mRNA, as well as CPT-1b and UCP-2 mRNA. SIRT1 and PGC-1α protein was reduced, and PPAR-γ protein increased. The addition of a PPAR-β agonist (L165,041) for the final 24 hours of 1 treatment resulted in increased levels of UCP-2 mRNA and protein whereas Rosiglitazone induced SIRT1, PGC-1α, RXR-α, PPAR-γ, CPT-1b, and UCP-2 mRNA and SIRT1 protein. Under hypoxia, Resveratrol induced SIRT1, RXR-, PPAR- mRNA, and PPAR- and UCP-2 protein. These findings demonstrate that hypoxia alters the components of the PPAR pathway involved in muscle fatty acid oxidative gene transcription and translation. These results have implications for understanding selective hypoxia adaptation and how it might impact long-term muscle oxidative metabolism and insulin sensitivity. Copyright © 2010 Timothy R. H. Regnault et al
Submergence of the Sidebands in the Photon-assisted Tunneling through a Quantum Dot Weakly Coupled to Luttinger Liquid Leads
We study theoretically the photon-assisted tunneling through a quantum dot
weakly coupled to Luttinger liquids (LL) leads, and find that the zero bias dc
conductance is strongly affected by the interactions in the LL leads. In
comparison with the system with Fermi liquid (FL) leads, the sideband peaks of
the dc conductance become blurring for 1/2<g<1, and finally merge into the
central peak for g<1/2, (g is the interaction parameter in the LL leads). The
sidebands are suppressed for LL leads with Coulomb interactions strong enough,
and the conductance always appears as a single peak for any strength and
frequency of the external time-dependent field. Furthermore, the quenching
effect of the central peak for the FL case does not exist for g<1/2.Comment: 9 pages, 4 figure
Accumulation of a polyisoprene-linked amino sugar in polymyxin-resistant Salmonella typhimurium and Escherichia coli: Structural characterization and transfer to lipid A in the periplasm
Polymyxin-resistant mutants of Escherichia coli and Salmonella typhimurium accumulate a novel minor lipid that can donate 4-amino-4-deoxy-L-arabinose units (L-Ara4N) to lipid A. We now report the purification of this lipid from a pss- pmrAC mutant of E. coli and assign its structure as undecaprenyl phosphate-α-L-Ara4N. Approximately 0.2 mg of homogeneous material was isolated from an 8-liter culture by solvent extraction, followed by chromatography on DEAE-cellulose, C18 reverse phase resin, and silicic acid. Matrix-assisted laser desorption ionization/time of flight mass spectrometry in the negative mode yielded a single species [M - H]- at m/z 977.5, consistent with undecaprenyl phosphate-α-L-Ara4N (Mr = 978.41). 31P NMR spectroscopy showed a single phosphorus atom at -0.44 ppm characteristic of a phosphodiester linkage. Selective inverse decoupling difference spectroscopy demonstrated that the undecaprenyl phosphate group is attached to the anomeric carbon of the L-Ara4N unit. One- and two-dimensional 1H NMR studies confirmed the presence of a polyisoprene chain and a sugar moiety with chemical shifts and coupling constants expected for an equatorially substituted arabinopyranoside. Heteronuclear multiple-quantum coherence spectroscopy analysis demonstrated that a nitrogen atom is attached to C-4 of the sugar residue. The purified donor supports in vitro conversion of lipid IVA to lipid IIA, which is substituted with a single L-Ara4N moiety. The identification of undecaprenyl phosphate-α -L-Ara4N implies that L-Ara4N transfer to lipid A occurs in the periplasm of polymyxin-resistant strains, and establishes a new enzymatic pathway by which Gram-negative bacteria acquire antibiotic resistance
Near-Limb Zeeman and Hanle Diagnostics
"Weak" magnetic-field diagnostics in faint objects near the bright solar disk
are discussed in terms of the level of non-object signatures, in particular, of
the stray light in telescopes. Calculated dependencies of the stray light
caused by diffraction at the 0.5-, 1.6-, and 4-meter entrance aperture are
presented. The requirements for micro-roughness of refractive and reflective
primary optics are compared. Several methods for reducing the stray light (the
Lyot coronagraphic technique, multiple stages of apodizing in the focal and
exit pupil planes, apodizing in the entrance aperture plane with a special
mask), and reducing the random and systematic errors are noted. An acceptable
level of stray light in telescopes is estimated for the V-profile recording
with a signal-to-noise ratio greater than three. Prospects for the limb
chromosphere magnetic measurements are indicated.Comment: 11 pages, 3 figure
Stabilization of Hydrodynamic Flows by Small Viscosity Variations
Motivated by the large effect of turbulent drag reduction by minute
concentrations of polymers we study the effects of a weakly space-dependent
viscosity on the stability of hydrodynamic flows. In a recent Letter [Phys.
Rev. Lett. {\bf 87}, 174501, (2001)] we exposed the crucial role played by a
localized region where the energy of fluctuations is produced by interactions
with the mean flow (the "critical layer"). We showed that a layer of weakly
space-dependent viscosity placed near the critical layer can have a very large
stabilizing effect on hydrodynamic fluctuations, retarding significantly the
onset of turbulence. In this paper we extend these observation in two
directions: first we show that the strong stabilization of the primary
instability is also obtained when the viscosity profile is realistic (inferred
from simulations of turbulent flows with a small concentration of polymers).
Second, we analyze the secondary instability (around the time-dependent primary
instability) and find similar strong stabilization. Since the secondary
instability develops around a time-dependent solution and is three-dimensional,
this brings us closer to the turbulent case. We reiterate that the large effect
is {\em not} due to a modified dissipation (as is assumed in some theories of
drag reduction), but due to reduced energy intake from the mean flow to the
fluctuations. We propose that similar physics act in turbulent drag reduction.Comment: 10 pages, 17 figs., REVTeX4, PRE, submitte
Representative diatom and coccolithophore species exhibit divergent responses throughout simulated upwelling cycles
Wind-driven upwelling followed by relaxation results in cycles of cold nutrient- rich water fueling intense phytoplankton blooms followed by nutrient depletion, bloom decline, and sinking of cells. Surviving cells at depth can then be vertically transported back to the surface with upwelled waters to seed another bloom. As a result of these cycles, phytoplankton communities in upwelling regions are transported through a wide range of light and nutrient conditions. Diatoms appear to be well suited for these cycles, but their responses to them remain understudied. To investigate the bases for diatoms' ecological success in upwelling environments, we employed laboratory simulations of a complete upwelling cycle with a common diatom, Chaetoceros decipiens, and coccolithophore, Emiliania huxleyi. We show that while both organisms exhibited physiological and transcriptomic plasticity, the diatom displayed a distinct response enabling it to rapidly shift-up growth rates and nitrate assimilation when returned to light and available nutrients following dark nutrient-deplete conditions. As observed in natural diatom communities, C. decipiens highly expresses before upwelling, or frontloads, key transcriptional and nitrate assimilation genes, coordinating its rapid response to upwelling conditions. Low-iron simulations showed that C. decipiens is capable of maintaining this response when iron is limiting to growth, whereas E. huxleyi is not. Differential expression between iron treatments further revealed specific genes used by each organism under low iron availability. Overall, these results highlight the responses of two dominant phytoplankton groups to upwelling cycles, providing insight into the mechanisms fueling diatom blooms during upwelling events
NTMG (N-terminal Truncated Mutants Generator for cDNA): an automatic multiplex PCR assays design for generating various N-terminal truncated cDNA mutants
The sequential deletion method is generally used to locate the functional domain of a protein. With this method, in order to find the various N-terminal truncated mutants, researchers have to investigate the ATG-like codons, to design various multiplex polymerase chain reaction (PCR) forward primers and to do several PCR experiments. This web server (N-terminal Truncated Mutants Generator for cDNA) will automatically generate groups of forward PCR primers and the corresponding reverse PCR primers that can be used in a single batch of a multiplex PCR experiment to extract the various N-terminal truncated mutants. This saves much time and money for those who use the sequential deletion method in their research. This server is available at http://oblab.cs.nchu.edu.tw:8080/WebSDL/
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