Last-male sperm precedence in Rhynchophorus ferrugineus (Olivier): observations in laboratory mating experiments with irradiated males

The Red PalmWeevil (RPW)Rhynchophorus ferrugineus(Olivier 1790) is an invasive pest from southeastern Asia and Melanesia that in the last 30 years has spread widely in the Middle East and Mediterranean Basin. Its stem-boring larvae cause great damage to several palm species of the Arecaceae family, many of which are economically important for agricultural and ornamental purposes. Therefore, great attention has recently been focused in studying this species to identify sustainable and effective eradication strategies, such as sterile insect technique (SIT). The rapid spread of RPW is associated with its high reproductive success. To evaluate the suitability of a SIT strategy, particular physiological and behavioral aspects of RPW reproduction, such as the presence of polyandry and post-copulatory sperm selection mechanisms, were investigated. To determine paternity of progeny from multiply mated females, double-crossing experiments were carried out confining individual females with either a wild-type male or a γ-irradiated male (Co-60). Fecundity and fertility of females were scored to evaluate post-copulatory sperm selection. Results showed that progeny were almost exclusively produced by the sperm of the second male, suggesting that a last-male sperm precedence is expressed at high levels in this species, and providing interesting insights for an area-wide RPW management strategy such as the SIT

SARS-CoV-2 Circulation in the School Setting: A Systematic Review and Meta-Analysis

The contribution of children to viral spread in schools is still debated. We conducted a systematic review and meta-analysis of studies to investigate SARS-CoV-2 transmission in the school setting. Literature searches on 15 May 2021 yielded a total of 1088 publications, including screening, contact tracing, and seroprevalence studies. MOOSE guidelines were followed, and data were analyzed using random-effects models. From screening studies involving more than 120,000 subjects, we estimated 0.31% (95% confidence interval (CI) 0.05–0.81) SARS-CoV-2 point prevalence in schools. Contact tracing studies, involving a total of 112,622 contacts of children and adults, showed that onward viral transmission was limited (2.54%, 95% CI 0.76–5.31). Young index cases were found to be 74% significantly less likely than adults to favor viral spread (odds ratio (OR) 0.26, 95% CI 0.11–0.63) and less susceptible to infection (OR 0.60; 95% CI 0.25–1.47). Lastly, from seroprevalence studies, with a total of 17,879 subjects involved, we estimated that children were 43% significantly less likely than adults to test positive for antibodies (OR 0.57, 95% CI 0.49–0.68). These findings may not applied to the Omicron phase, we further planned a randomized controlled trial to verify these results

SARS-CoV-2 Circulation in the School Setting: A Systematic Review and Meta-Analysis

The contribution of children to viral spread in schools is still debated. We conducted a systematic review and meta-analysis of studies to investigate SARS-CoV-2 transmission in the school setting. Literature searches on 15 May 2021 yielded a total of 1088 publications, including screening, contact tracing, and seroprevalence studies. MOOSE guidelines were followed, and data were analyzed using random-effects models. From screening studies involving more than 120,000 subjects, we estimated 0.31% (95% confidence interval (CI) 0.05–0.81) SARS-CoV-2 point prevalence in schools. Contact tracing studies, involving a total of 112,622 contacts of children and adults, showed that onward viral transmission was limited (2.54%, 95% CI 0.76–5.31). Young index cases were found to be 74% significantly less likely than adults to favor viral spread (odds ratio (OR) 0.26, 95% CI 0.11–0.63) and less susceptible to infection (OR 0.60; 95% CI 0.25–1.47). Lastly, from seroprevalence studies, with a total of 17,879 subjects involved, we estimated that children were 43% significantly less likely than adults to test positive for antibodies (OR 0.57, 95% CI 0.49–0.68). These findings may not applied to the Omicron phase, we further planned a randomized controlled trial to verify these results

Prognostic Role of CSF β-amyloid 1-42/1-40 Ratio in Patients Affected by Amyotrophic Lateral Sclerosis

The involvement of β-amyloid (Aβ) in the pathogenesis of amyotrophic lateral sclerosis (ALS) has been widely discussed and its role in the disease is still a matter of debate. Aβ accumulates in the cortex and the anterior horn neurons of ALS patients and seems to affect their survival. To clarify the role of cerebrospinal fluid (CSF) Aβ 1-42 and Aβ 42/40 ratios as a potential prognostic biomarker for ALS, we performed a retrospective observational study on a cohort of ALS patients who underwent a lumbar puncture at the time of the diagnosis. CSF Aβ 1-40 and Aβ 1-42 ratios were detected by chemiluminescence immunoassay and their values were correlated with clinical features. We found a significant correlation of the Aβ 42/40 ratio with age at onset and Mini Mental State Examination (MMSE) scores. No significant correlation of Aβ 1-42 or Aβ 42/40 ratios to the rate of progression of the disease were found. Furthermore, when we stratified patients according to Aβ 1-42 concentration and the Aβ 42/40 ratio, we found that patients with a lower Aβ 42/40 ratio showed a shorter survival. Our results support the hypothesis that Aβ 1-42 could be involved in some pathogenic mechanism of ALS and we suggest the Aβ 42/40 ratio as a potential prognostic biomarker

An active region filament studied simultaneously in the chromosphere and photosphere: I - Magnetic structure

A thorough multiwavelength, multiheight study of the vector magnetic field in a compact active region (AR) filament (NOAA10781) is presented. We suggest an evolutionary scenario for this filament. Full Stokes vectors were acquired with TIP-II in a spectral range which comprises the chromospheric He I 10830 A multiplet and the photospheric Si I 10827 A line. An AR filament (that was formed before our observing run) was detected in the He I absorption images on 2005 July 3rd. The chromospheric vector magnetic field in this portion of the filament was strongly sheared whereas the photospheric field lines underneath had an inverse polarity configuration. From July 3rd to July 5th, an opening and closing of the polarities at either side of the polarity inversion line (PIL) was recorded, resembling the recently discovered process of the sliding door effect seen by Hinode. During this time, a newly created region that contained pores and orphan penumbrae at the PIL was observed.On July 5th, a normal polarity configuration was inferred from the chromospheric spectra, while strongly sheared field lines aligned with the PIL were found in the photosphere. In this same data set, the spine of the filament is also observed in a different portion of the FOV and is clearly mapped by the Silicon line core. The inferred vector magnetic fields of the filament suggest a flux rope topology. Furthermore, the observations indicate that the filament is divided in two parts, one which lies in the chromosphere and another one that stays trapped in the photosphere. Therefore, only the top of the helical structure is seen by the Helium lines. The pores and orphan penumbrae at the PIL appear to be the photospheric counterpart of the extremely low-lying filament. We suggest that orphan penumbrae are formed in very narrow PILs of compact ARs and are the photospheric manifestation of flux ropes in the photosphere.Comment: Accepted for publication in Astronomy & Astrophysics, 16 pages, 13 figure

Scintigraphic evaluation of oesophageal transit during radiotherapy to the mediastinum

Background: To quantitatively evaluate radiation-induced impaired oesophageal transit with oesophageal transit scintigraphy and to assess the relationships between acute oesophagitis symptoms and dysmotility.\ud \ud Methods: Between January 1996 and November 1998, 11 patients affected by non-small-cell carcinoma of the lung not directly involving the oesophagus, requiring adjuvant external beam radiotherapy (RT) to the mediastinum were enrolled. Oesophageal transit scans with liquid and semisolid bolus were performed at three pre-defined times: before (T0) and during radiation at 10 Gy (T1) and 30 Gy (T2). Two parameters were obtained for evaluation: 1) mean transit time (MTT); and 2) ratio between peak activity and residual activity at 40 seconds (ER-40s). Acute radiation toxicity was scored according to the joint EORTC-RTOG criteria. Mean values with standard deviation were calculated for all parameters. Analysis of variance (ANOVA) tests and paired t-Tests for all values were performed.\ud \ud Results: An increase in the ER-40s from T0 to T1 or T2 was seen in 9 of 11 patients (82%). The mean ER-40s value for all patients increased from 0.8306 (T0) to 0.8612 (T1) and 0.8658 (T2). These differences were statistically significant (p < 0.05) in two paired t-Tests at T0 versus T2 time: overall mean ER-40s and upright ER-40s (p = 0.041 and p = 0.032, respectively). Seven patients (63%) showed a slight increase in the mean MTT value during irradiation but no statistically significant differences in MTT parameters were found between T0, T1 and T2 (p > 0.05).\ud \ud Conclusion: Using oesophageal scintigraphy we were able to detect early alterations of oesophageal transit during the third week of thoracic RT

Prominence eruption observed in He II 304 Å up to >6 R⊙ by EUI/FSI aboard Solar Orbiter⋆

Aims. We report observations of a unique, large prominence eruption that was observed in the He II 304 Å passband of the Extreme Ultraviolet Imager/Full Sun Imager telescope aboard Solar Orbiter on 15–16 February 2022. Methods. Observations from several vantage points – Solar Orbiter, the Solar-Terrestrial Relations Observatory, the Solar and Heliospheric Observatory, and Earth-orbiting satellites – were used to measure the kinematics of the erupting prominence and the associated coronal mass ejection. Three-dimensional reconstruction was used to calculate the deprojected positions and speeds of different parts of the prominence. Observations in several passbands allowed us to analyse the radiative properties of the erupting prominence. Results. The leading parts of the erupting prominence and the leading edge of the corresponding coronal mass ejection propagate at speeds of around 1700 km s−1 and 2200 km s−1, respectively, while the trailing parts of the prominence are significantly slower (around 500 km s−1). Parts of the prominence are tracked up to heights of over 6 R⊙. The He II emission is probably produced via collisional excitation rather than scattering. Surprisingly, the brightness of a trailing feature increases with height. Conclusions. The reported prominence is the first observed in He II 304 Å emission at such a great height (above 6 R⊙)