Frequencies Of Virulence Genes And Pulse Field Gel Electrophoresis Fingerprints In Escherichia Coli Isolates From Canine Pyometra

Escherichia coli is the most common bacterial agent isolated from canine pyometra. The frequencies of 24 virulence genes and pulsed field gel electrophoresis (PFGE) profiles were determined for 23 E. coli isolates from cases of canine pyometra in Brazil. The frequencies of virulence genes were 91.3% fimH, 91.3% irp-2, 82.6% fyuA, 56.5% iroN, 47.8% traT, 39.1% usp, 34.8% sfaD/E, 34.8% tsh, 30.4% papC, 30.4% hlyA, 26.1% papGIII, 26.1% cnf-1, 21.7% papE/F, 21.7% iss, 17.4% iutA, 17.4% ompT, 17.4% cvaC, 17.4% hlyF, 17.4% iucD, 13.0% iucC, 13.0% astA, 4.3% papGII, 0% afaB/C and 0% papGI. The high frequency of yersiniabactin (fyuA and irp2) and salmochelin (iroN) genes suggests that iron uptake systems might be important in the pathogenesis of canine pyometra. PFGE profiles of 19 isolates were heterogeneous, confirming that E. coli isolates from canine pyometra are unlikely to be epidemic clones.2022393395Chen, Y.M.M., Wright, P.J., Lee, C.-S., Browning, G.F., Uropathogenic virulence factors in isolates of Escherichia coli from clinical cases of canine pyometra and feces of healthy bitches (2003) Veterinary Microbiology, 94, pp. 57-69Coggan, J.A., Melville, P.A., Oliveira, C.M., Faustino, M., Moreno, A.M., Benites, N.R., Microbiological and histopathological aspects of canine pyometra (2008) Brazilian Journal of Microbiology, 39, pp. 477-483Ghanbarpour, R., Akhtardanesh, B., Genotype and antibiotic resistance profile of Escherichia coli strains involved in canine pyometra (2012) Comparative Clinical Pathology, 21, pp. 737-744Hagman, R., Kühn, I., Escherichia coli strains isolated from the uterus and urinary bladder of bitches suffering from pyometra: Comparison by restriction enzyme digestion and pulsed-field gel electrophoresis (2002) Veterinary Microbiology, 84, pp. 143-153Krekeler, N., Marenda, M.S., Browning, G.F., Holden, K.M., Charles, J.A., Wright, P.J., The role of type 1, P and S fimbriae in binding of Escherichia coli to the canine endometrium (2013) Veterinary Microbiology, 164, pp. 399-404Mateus, L., Henriques, S., Merino, C., Pomba, C., Lopes da Costa, L., Silva, E., Virulence genotypes of Escherichia coli canine isolates from pyometra, cystitis and fecal origin (2013) Veterinary Microbiology, 166, pp. 590-594Ribot, E.M., Fair, M.A., Gautom, R., Cameron, D.N., Hunter, S.B., Swaminathan, B., Barrett, T.J., Standardization of pulsed-field gel electrophoresis protocols for the subtyping of Escherichia coli O157:H7, Salmonella, and Shigella for PulseNet (2006) Foodborne Pathogens and Disease, 3, pp. 59-67Siqueira, A.K., Ribeiro, M.G., Leite, D.D.S., Tiba, M.R., Moura, C.D., Lopes, M.D., Prestes, N.C., de Silva, A.V., Virulence factors in Escherichia coli strains isolated from urinary tract infection and pyometra cases and from feces of healthy dogs (2009) Research in Veterinary Science, 86, pp. 206-210Smith, F.O., Canine pyometra (2006) Theriogenology, 66, pp. 610-612Wadås, B., Kühn, I., Lagerstedt, A.-S., Jonsson, P., Biochemical phenotypes of Escherichia coli in dogs: Comparison of isolates isolated from bitches suffering from pyometra and urinary tract infection with isolates from faeces of healthy dogs (1996) Veterinary Microbiology, 52, pp. 293-30