Growth parameters and yield attributing characters of PR-118 (V1) and PR-116 (V2) varieties of rice (Oryza sativa L.) as influenced by different planting methods

Field experiments were conducted at Punjab Agricultural University, Ludhiana (Punjab) to study the effect of different agronomic aspects of bed planting on growth and yield of rice during Kharif seasons of 2012 and 2013. The 30 days old seedlings of both rice varieties PR-118 (V1) and PR-116 (V2) were transplanted on 15th June (D1), 30th June (D2) and 15th July (D3). The two rice varieties were transplanted under bed planting (M1) and conventional planting (M2) methods respectively. The results showed that growth parameters like number of tillers per plant, dry weight per plant leaf area index (LAI) and plant height were significantly higher in bed planting than conventional method. In bed planting method, grain yield of rice (48.82q/h) was found to be more than the conventional method (35.74 q/h) during 2012.Varieties PR-118 yielded 47.61q/h more than PR-116 (39.97 q/h) in bed planting. Yield contributing characters like number of effective tillers, number of grains per plant and 1000-grain weight of rice were more in bed planting than conventional method. Harvest index and biological yield was also found to be more in bed planting method than conventional planting. Rice transplanted on 15th June yielded (50.15q/h) more than 30th June (41.45q/h) and 15th July (35.27q/h). Similar results were found in Kharif 2013. Interaction between dates of transplanting and varieties and between varieties and planting methods were found significant

Role of agronomic manipulations in modification of wheat microclimate under central Punjab

Wheat crop is influenced by different microclimatic parameters like solar radiation, canopy temperature etc. Agronomic manipulation like change in row spacing and row direction can be used as a strategy to modify the microclimate of crop. Keeping these facts in view, field trials were conducted during rabi 2012-13 and 2013-14 under two experiments in first experiment wheat varieties HD 2967, PBW 550 and PBW 343 were sown under three row spacing viz. 15 cm, 22.5 cm and 30 cm. In second experiment, wheat varieties HD 2967, PBW 550 and PBW 343 were sown under two row direction viz. North-South (N-S) and East-West (E-W). Short wave radiation interception and canopy temperature was recorded under different treatments at 15 days interval. Among different row spacing, short wave radiation interception and canopy temperature was maximum at 30 cm row spacing (77.7% and 25.1oC) followed by 22.5 cm (75.7% and 24.2oC) and 15 cm row spacing (73.9% and 23.2oC), whereas under row directions short wave radiation interception and canopy temperature was more (76.5% and 23.9oC) in E-W row direction as compared to N-S row direction (75% and 23.2oC). Relationships were developed between dry matter accumulation and canopy temperature. Polynomial relationships gave significant R2 value (0.66 & 0.69) under different treatments. This two year study indicated that agronomic manipulations play an important role in microclimate modification and canopy temperature significantly influence dry matter accumulation under different crop geometry

Acetolactate synthase regulatory subunits play divergent and overlapping roles in branched-chain amino acid synthesis and Arabidopsis development

Branched-chain amino acids (BCAAs) are synthesized by plants, fungi, bacteria, and archaea with plants being the major source of these amino acids in animal diets. Acetolactate synthase (ALS) is the first enzyme in the BCAA synthesis pathway. Although the functional contribution of ALS to BCAA biosynthesis has been extensively characterized, a comprehensive understanding of the regulation of this pathway at the molecular level is still lacking

Characterization of Biofilm Formation by Borrelia burgdorferi In Vitro

Borrelia burgdorferi, the causative agent of Lyme disease, has long been known to be capable of forming aggregates and colonies. It was recently demonstrated that Borrelia burgdorferi aggregate formation dramatically changes the in vitro response to hostile environments by this pathogen. In this study, we investigated the hypothesis that these aggregates are indeed biofilms, structures whose resistance to unfavorable conditions are well documented. We studied Borrelia burgdorferi for several known hallmark features of biofilm, including structural rearrangements in the aggregates, variations in development on various substrate matrices and secretion of a protective extracellular polymeric substance (EPS) matrix using several modes of microscopic, cell and molecular biology techniques. The atomic force microscopic results provided evidence that multilevel rearrangements take place at different stages of aggregate development, producing a complex, continuously rearranging structure. Our results also demonstrated that Borrelia burgdorferi is capable of developing aggregates on different abiotic and biotic substrates, and is also capable of forming floating aggregates. Analyzing the extracellular substance of the aggregates for potential exopolysaccharides revealed the existence of both sulfated and non-sulfated/carboxylated substrates, predominately composed of an alginate with calcium and extracellular DNA present. In summary, we have found substantial evidence that Borrelia burgdorferi is capable of forming biofilm in vitro. Biofilm formation by Borrelia species might play an important role in their survival in diverse environmental conditions by providing refuge to individual cells

A systematic review of the effectiveness and cost-effectiveness of peer education and peer support in prisons.

BACKGROUND: Prisoners experience significantly worse health than the general population. This review examines the effectiveness and cost-effectiveness of peer interventions in prison settings. METHODS: A mixed methods systematic review of effectiveness and cost-effectiveness studies, including qualitative and quantitative synthesis was conducted. In addition to grey literature identified and searches of websites, nineteen electronic databases were searched from 1985 to 2012. Study selection criteria were: Population: Prisoners resident in adult prisons and children resident in Young Offender Institutions (YOIs). INTERVENTION: Peer-based interventions Comparators: Review questions 3 and 4 compared peer and professionally led approaches. OUTCOMES: Prisoner health or determinants of health; organisational/ process outcomes; views of prison populations. STUDY DESIGNS: Quantitative, qualitative and mixed method evaluations. RESULTS: Fifty-seven studies were included in the effectiveness review and one study in the cost-effectiveness review; most were of poor methodological quality. Evidence suggested that peer education interventions are effective at reducing risky behaviours, and that peer support services are acceptable within the prison environment and have a positive effect on recipients, practically or emotionally. Consistent evidence from many, predominantly qualitative, studies, suggested that being a peer deliverer was associated with positive effects. There was little evidence on cost-effectiveness of peer-based interventions. CONCLUSIONS: There is consistent evidence from a large number of studies that being a peer worker is associated with positive health; peer support services are also an acceptable source of help within the prison environment and can have a positive effect on recipients. Research into cost-effectiveness is sparse. SYSTEMATIC REVIEW REGISTRATION: PROSPERO ref: CRD42012002349

Cellular Cytoskeleton Dynamics Modulates Non-Viral Gene Delivery through RhoGTPases

Although it is well accepted that the constituents of the cellular microenvironment modulate a myriad of cellular processes, including cell morphology, cytoskeletal dynamics and uptake pathways, the underlying mechanism of how these pathways influence non-viral gene transfer have not been studied. Transgene expression is increased on fibronectin (Fn) coated surfaces as a consequence of increased proliferation, cell spreading and active engagement of clathrin endocytosis pathway. RhoGTPases mediate the crosstalk between the cell and Fn, and regulate cellular processes involving filamentous actin, in-response to cellular interaction with Fn. Here the role of RhoGTPases specifically Rho, Rac and Cdc42 in modulation of non-viral gene transfer in mouse mesenchymal stem (mMSCs) plated in a fibronectin microenvironment was studied. More than 90% decrease in transgene expression was observed after inactivation of RhoGTPases using difficile toxin B (TcdB) and C3 transferase. Expression of dominant negative RhoA (RhoAT19N), Rac1(Rac1T17N) and Cdc42 (Cdc42T17N) also significantly reduced polyplex uptake and transgene expression. Interactions of cells with Fn lead to activation of RhoGTPases. However, further activation of RhoA, Rac1 and Cdc42 by expression of constitutively active genes (RhoAQ63L, Rac1Q61L and Cdc42Q61L) did not further enhance transgene expression in mMSCs, when plated on Fn. In contrast, activation of RhoA, Rac1 and Cdc42 by expression of constitutively active genes for cells plated on collagen I, which by itself did not increase RhoGTPase activation, resulted in enhanced transgene expression. Our study shows that RhoGTPases regulate internalization and effective intracellular processing of polyplexes that results in efficient gene transfer