An Analysis of Cabin Ozone Regulations

Exposure to elevated levels of ozone have been reported to be associated with complaints of discomfort such as dry mouth, eye irritation and dryness, nasal irritation coughing, and headaches. The Federal Aviation Administration (FAA) established regulatory requirements in 1980 to limit cabin ozone levels to no more than 0.25 parts per million (ppm) at any time or 0.1 ppm averaged over a 3-hour interval for any flight over four hours in length. The FAA also published an Advisory Circular (AC), AC 120-38, to provide guidance to air carriers on how to comply with these then new ozone regulations. Methods of compliance include the use of catalytic converters, or ozone filters, designed to remove ozone, utilizing statistical methods to prove that ozone concentrations will not exceed limits for the carrier’s route structure and flight planning to avoid areas of reported high concentrations of ozone. The calculations used to determine cabin ozone concentration from manufacturer’s filter efficiency data and ozone levels are to be based on published ozonesonde data found in the AC 120-38 or an equivalent data set. Unfortunately, the published ozonesonde data in the AC 120-38 are outdated and the AC does not point to any other data source that is acceptable to the FAA to conduct the required statistical analysis. In addition, once compliance is shown, no followup measurements are required to ensure that ozone levels remain below these required levels. Actual ozone concentrations have been measured in the aircraft by several researchers that exceed these regulatory levels. Finally, FAA ozone regulations and AC 120-38 do not address cumulative effects of ozone exposure to crewmembers over multiple flights and do not offer any protection against ozone exposure for crewmembers on non-passenger carrying flights. A revision of federal regulations to afford protection to all crewmembers, account for cumulative effects, and updated compliance methods that rely on current ozonesonde data and periodic ozone monitoring should be accomplished to ensure crewmembers are not subjected to ozone levels that could potentially result in serious health concerns

Registration is Fundamental

Under the Copyright Act, copyright owners can file infringement actions only if registration of their copyright claim with the Copyright Office “has been made” or “has been refused.” The United States Supreme Court recently granted certiorari in Fourth Estate v. Wall-Street.com, in order to decide whether registration is “made” when a claimant files a registration application or when the Copyright Office registers the claim. This article argues that the Court should hold that registration occurs when the Copyright Office registers the claim, in order to ensure that federal courts can benefit from the expertise of the Copyright Office. The Copyright Office recently began publishing the opinions of Copyright Office Review Board. This article uses those administrative opinions to show how the Copyright Office has developed the concepts of originality and creativity in ways that are helpful to the federal courts. It concludes with an Appendix listing the Copyright Office Review Board opinions addressing originality and explaining the basis for each decision

The Effects of Acute and Chronic Hypoxia on Cortisol, Glucose and Lactate Concentrations in Different Populations of Three-Spined Stickleback

The response of individuals from three different populations of three-spined sticklebacks to acute and chronic periods of hypoxia (4.4 kPa DO, 2.2 mg l-1) were tested using measures of whole-body (WB) cortisol, glucose and lactate. Although there was no evidence of a neuroendocrine stress response to acute hypoxia, fish from the population least likely to experience hypoxia in their native habitat had the largest response to low oxygen, with significant evidence of anaerobic glycolysis after two hours of hypoxia. However, there was no measurable effect of a more prolonged period (seven days) of hypoxia on any of the fish in this study, suggesting that they acclimated to this low level of oxygen over time. Between-population differences in the analytes tested were observed in the control fish of the acute hypoxia trial, which had been in the laboratory for 16 days. However, these differences were not apparent among the control fish in the chronic exposure groups that had been held in the laboratory for 23 days suggesting that these site-specific trends in physiological status were acclimatory. Overall, the results of this study suggest that local environmental conditions may shape sticklebacks’ general physiological profile as well as influencing their response to hypoxia

Electrically Mediated Plasmid DNA Delivery to Hepatocellular Carcinomas in Vivo

Gene therapy by direct delivery of plasmid DNA has several advantages over viral gene transfer, but plasmid delivery is less efficient. In vivo electroporation has been used to enhance delivery of chemotherapeutic agents to tumors in both animal and human studies. Recently, this delivery technique has been extended to large molecules such as plasmid DNA. Here, the successful delivery of plasmids encoding reporter genes to rat hepatocellular carcinomas by in vivo electroporation is demonstrated

A frequency weighting for the evaluation of steering wheel rotational vibration

The human perception of rotational hand-arm vibration has been investigated by means of a test rig consisting of a rigid frame, an electrodynamic shaker unit, a rigid steering wheel, a shaft assembly, bearings and an automobile seat. Fifteen subjects were tested while seated in a driving posture. Four equal sensation tests and one annoyance threshold test were performed using sinusoidal excitation at 18 frequencies in the range from 3 to 315 Hz. In order to guarantee the generality of the equal sensation data the four tests were defined to permit checks of the possible influence of three factors: reference signal amplitude, psychophysical test procedure and temporary threshold shift (TTSv) caused by the test exposure. All equal sens ation tests used a reference sinusoid of 63 Hz at either 1.0 or 1.5 m/s2 r.m.s. in amplitude. The four equal sensation curves were similar in shape and suggested a decrease in human sensitivity to hand-arm rotational vibration with increasing frequency. The slopes of the equal sensation curves changed at transition points of approximately 6.3 and 63 Hz. A frequency weighting, called Ws, was developed for the purpose of evaluating steering wheel rotational vibration. The proposed Ws has a slope of 0 dB per octave over the frequency range from 3 to 6.3 Hz, a slope of -6 dB per octave from 6.3 to 50 Hz, a slope of 0 dB per octave from 50 to 160 Hz and a slope of -10 dB per octave from 160 to 315 Hz. Ws provides a possible alternative to the existing Wh frequency weighting defined in International Standards Organisation 5349-1 (2001) and British Standards Institution 6842 (1987)

Does environmental enrichment promote recovery from stress in rainbow trout?

The EC Directive on animal experimentation suggests that animals should have enrichment to improve welfare yet relatively little research has been conducted on the impact of enrichment in fish. Studies on zebrafish have been contradictory and other fish species may require species specific enrichments. Salmonids are important experimental models given their relevance to aquaculture and natural ecosystems. This study sought to establish how an enriched environment may promote better welfare in rainbow trout (Oncorhynchus mykiss) enhancing their recovery from invasive procedures. Trout were held individually in either barren or enriched (gravel, plants and an area of cover) conditions and recovery rates after a potentially painful event and a standard stressor were investigated by recording parameters such as behaviour, opercular beat rate and plasma cortisol concentrations. Fish were randomly assigned to one of four treatment groups: Control where the fish were left undisturbed; Sham where fish were anaesthetised but no invasive procedure; Pain where a subcutaneous injection of acetic acid was administered to the frontal lips during anaesthesia; and Stress where fish were subject to one minute of air emersion. Video recordings were made prior to treatment then at 30 minute intervals afterwards to determine whether fish in enriched conditions recovered more rapidly than those in barren tanks. Preliminary analyses suggest that enriched fish may be less stressed thus these findings have important implications for the husbandry and welfare of captive rainbow trout but may also affect the outcome of experimental studies dependent upon whether enrichment was adopted

MOspace training session for University of Missouri-St. Louis

Please note that there are several files associated with this item: A pdf version of the slides presented ; The audio recording of the session (in two parts) ; A slidecast (slides with audio) of the session in two parts; note that clicking on the file called MOspaceTrainingUMSLStream1 or MOspaceTrainingUMSLStream2 will launch a browser window to present the content.This presentation introduced the audience to MOspace, the University of Missouri's open access digital institutional repository and how to use it

The National Cancer Institute Cohort Consortium : An International Pooling Collaboration of 58 Cohorts from 20 Countries

Cohort studies have been central to the establishment of the known causes of cancer. To dissect cancer etiology in more detail-for instance, for personalized risk prediction and prevention, assessment of risks of subtypes of cancer, and assessment of small elevations in risk-there is a need for analyses of far larger cohort datasets than available in individual existing studies. To address these challenges, the NCI Cohort Consortium was founded in 2001. It brings together 58 cancer epidemiology cohorts from 20 countries to undertake large-scale pooling research. The cohorts in aggregate include over nine million study participants, with biospecimens available for about two million of these. Research in the Consortium is undertaken by >40 working groups focused on specific cancer sites, exposures, or other research areas. More than 180 publications have resulted from the Consortium, mainly on genetic and other cancer epidemiology, with high citation rates. This article describes the foundation of the Consortium; its structure, governance, and methods of working; the participating cohorts; publications; and opportunities. The Consortium welcomes newmembers with cancer-oriented cohorts of 10,000 or more participants and an interest in collaborative research. (C) 2018 AACR.Peer reviewe