495 research outputs found
Testing demand responsive shared transport services via agent-based simulations
Demand Responsive Shared Transport DRST services take advantage of
Information and Communication Technologies ICT, to provide on demand transport
services booking in real time a ride on a shared vehicle. In this paper, an
agent-based model ABM is presented to test different the feasibility of
different service configurations in a real context. First results show the
impact of route choice strategy on the system performance
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Extensive alternative polyadenylation during zebrafish development
The post-transcriptional fate of messenger RNAs (mRNAs) is largely dictated by their 3′ untranslated regions (3′ UTRs), which are defined by cleavage and polyadenylation (CPA) of pre-mRNAs. We used poly(A)-position profiling by sequencing (3P-seq) to map poly(A) sites at eight developmental stages and tissues in the zebrafish. Analysis of over 60 million 3P-seq reads substantially increased and improved existing 3′ UTR annotations, resulting in confidently identified 3′ UTRs for >79% of the annotated protein-coding genes in zebrafish. mRNAs from most zebrafish genes undergo alternative CPA, with those from more than a thousand genes using different dominant 3′ UTRs at different stages. These included one of the poly(A) polymerase genes, for which alternative CPA reinforces its repression in the ovary. 3′ UTRs tend to be shortest in the ovaries and longest in the brain. Isoforms with some of the shortest 3′ UTRs are highly expressed in the ovary, yet absent in the maternally contributed RNAs of the embryo, perhaps because their 3′ UTRs are too short to accommodate a uridine-rich motif required for stability of the maternal mRNA. At 2 h post-fertilization, thousands of unique poly(A) sites appear at locations lacking a typical polyadenylation signal, which suggests a wave of widespread cytoplasmic polyadenylation of mRNA degradation intermediates. Our insights into the identities, formation, and evolution of zebrafish 3′ UTRs provide a resource for studying gene regulation during vertebrate development.National Institutes of Health (U.S.) (Grant GM067031)
T-cell depleted HLA-haploidentical HSCT in a child with neuromyelitis optica
Neuromyelitis optica is an immune-mediated disease characterized by a relapsing course, resulting in progressive disability. In children, given the long life expectancy, a disease-modifying treatment could be particularly desirable. Unfortunately, the currently available treatment strategies with this potential are scarce. Very limited data are available about the use of allogeneic hematopoietic stem cell transplantation (HSCT) for autoimmune neurological diseases. In this report, we present a pediatric case successfully treated with allogeneic HSCT from an HLA-haploidentical donor, after ex vivo TCR/CD19-depletion of the graft. To the best of our knowledge, this is the first case of a pediatric patient to benefit from such a treatment
Vortex dynamics in NbTi films at high frequency and high DC magnetic fields
We report on the characterization of NbTi films at ∼ 11 GHz and in DC magnetic fields up to 4
T, performed by means of the coplanar waveguide resonator technique, providing quantitative
information about the penetration depth, the complex impedance, and the vortex-motion-induced
complex resistivity. This kind of characterization is essential for the development of radiofrequency
cavity technology. To access the vortex-pinning parameters, the complex impedance was analyzed
within the formalism of the Campbell penetration depth. Measurements in this frequency range
allowed us to determine the complete set of vortex-pinning parameters and the flux flow resistivity,
both analyzed and discussed in the framework of high-frequency vortex dynamics models. The
analysis also benefits from the comparison with results obtained by a dielectric-loaded resonator
technique on similar samples and by other ancillary structural and electromagnetic characterization
techniques that provide us with a comprehensive picture of the material. It turns out that the
normalized flux flow resistivity follows remarkably well the trend predicted by the time dependent
Ginzburg-Landau theory, while the pinning constant exhibits a decreasing trend with the field which
points to a collective pinning regime
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